Phf14 (BC040236) Mouse Tagged ORF Clone

CAT#: MR211047

Phf14 (Myc-DDK-tagged) - Mouse PHD finger protein 14 (cDNA clone MGC:36262 IMAGE:3585881)

Product Images

Circular map for MR211047

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phf14
• Synonyms: 1110001C23Rik; 4932409F11Rik; 5730446A07Rik; AA623952; AV297001; mKIAA0783
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211047 representing BC040236
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGCAGCTCCAAGAGGAGGCAGGTGAAGCCTTTGGCCGCTTCTCTCCTGGAAGCTCTTGATTATG
  ATAGTTCAGATGACAGTGATTTTAAAGTTGGAGATGCTTCAGATTCTGAAGGGAGTGGTAATGGAAGTGA
  AGATCCTTCAAAGGACAGTGGAGAAGGTTCCTGTAGTGATTCTGAGGAAAACATTTTAGAAGAAGAACTG
  AATGAAGATATTCAAGTAAAAGAAGAACAGCTTAAAAATTCTACAGAGGAAATAATGCCATCAGACAAAC
  AGTTAATTAAAATGGAAAAGAAGGAAGAAGAAGAAAACGGAGAAAGACCTAGAAAGAAAAAGGAGAAAGA
  GAAGGAGAAAGAAAAGGAAAGGGAGAAGGACAAAGAGAAAGCAACAATATCTGATAGTGCAGCTGCTTCT
  GCTGCTGGCACCACACCAGCCACAAGCCCTCCTGCTGTCACCTCCCCTTCTGTCCCTACCACCACTACCA
  CCACCACGGAGGAACAAGTCAGTGAGCCCAAGAAATGGAATCTTCGTCGGAACCGTCCCCTGTTGGACTT
  TGTATCCATGGAAGAACTCAATGCTATGGATGATTATGACAGTGAGGATGATAATGACTGGAGACCTACT
  GTAGTAAAGAGAAAGGGGCGATCAGCGTCTCAGAAGGAGGGCAGTGATGGAGACAATGAGGATGATGACG
  ATGAGGGAAGTGGGAGTGAGGAAGATGAGAATGATGAGGGCAATGATGAAGATCATAGCAGCCCTGCTAG
  CGAAGCAGGTGGCAAGAAGAAGAGGAGTAAAGTTCTTAGCAGAAACAGTGCTGATGATGAGGAACTGACC
  AATGATAGCCTGACCCTATCTCAAAGCAAGAGTAATGAGGACTCTCTGATTCTTGAGAAGAGTCAAAATT
  GGAGTTCTCAGAAAATGGACCACATTCTGATTTGCTGTGTTTGTCTTGGAGATAATAGTGAAGATGCAGA
  TGAAATAATTCAGTGTGATAATTGTGGCATTACAGTTCATGAAGGTTGTTATGGAGTCGATGGGGAGAGT
  GACTCTATTATGAGTTCGGCTTCTGAAAACTCCACTGAGCCTTGGTTTTGTGATGCCTGTAAATGTGGTG
  TATCTCCTAGCTGTGAACTATGTCCTAATCAGGATGGGATTTTCAAGGAGACGGATGCTGGAAGATGGGT
  CCACATTGTTTGTGCCCTATATGTTCCTGGAGTGGCTTTTGGAGATATTGATAAATTACGACCAGTAACG
  CTAACAGAAATGAACTATTCTAAATATGGTGCCAAGGAGTGTAGCTTTTGTGAAGATCCTCGCTTTGCTA
  GAACTGGAGTTTGCATTAGCTGTGATGCAGGCATGTGTAGAGCCTATTTCCATGTCACCTGTGCCCAAAA
  GGAAGGCCTGCTATCAGAGGCAGCAGCAGAAGAGGATATAGCAGATCCATTTTTTGCTTATTGTAAGCAG
  CATGCAGACAGATTAGACAGAAAATGGAAAAGAAAAAACTACTTGGCTCTACAATCCTATTGTAAAATGT
  CTTTGCAAGAGAGAGAAAAACAGCTGTCCCCTGAAGCACAGGCAAGGATCAATGCCCGACTACAGCAATA
  TCGTGCCAAGGCAGAGCTGGCGCGATCTACCAGACCACAAGCCTGGGTTCCAAGGGAAAAGCTACCCAGG
  CCACTGACAAGTAGTGCTTCGGCTATTCGTAAGCTTATGCGCAAAGCAGAGCTGATGGGAATCAGTACGG
  ATATCTTTCCAGTGGACAACTCAGATACTAGTTCTAGTGTGGATGGAAGGCGGAAGCATAAGCAGCCCGC
  TCTCACTGCTGATTTTGTGAATTACTATTTTGAGAGAAATATGCGCATGATTCAAATTCAGGAAAATATG
  GCTGAACAAAAGAATATAAAGGATAAATTAGAGAATGAGCAAGAAAAGCTTCATGTAGAATATAATAAGC
  TCTGTGAATCATTAGAGGAACTGCAAAATCTGAATGGGAAACTTCGAAGTGAAGGGCAAGGGATATGGGC
  CTTACTTGGCCGAATTACAGGGCAGAAGTTGAATGTACCAGCAATCTTACGAGCACCTAAGGAAAGGAAA
  CCAAGTAAAAAAGAAGGAGGCACACAAAAGACATCTGCTCTTCCTACAGTGCTTTATAGTTGTGGAATTT
  GTAAGAAGAACCATGATCAGCATCTTCTTTTACTGTGTGACACCTGTAAACTGCATTATCATCTCGGCTG
  CCTTGATCCTCCTCTTACAAGGATGCCGAGAAAGACGAAAAACAGTTACTGGCAATGCTCAGAGTGTGAC
  CAGGCAGGGAGCAGCGACATGGAAGCAGAGATGGCCATGGAAACCTTACCAGATGGAACAAAACGATCAA
  GGAGACAGATTAAGGAGCCAGTGAAGTTTGTGCCACAGGATGTCCCACCAGAACCCAAGAAGATTCCAAT
  AAGAAACACGAGAACCAGAGGACGAAAGCGAAGCTTTGTTCCTGAGGAAGAAAAACATGAGGAAAGAGTT
  CCTAGGGAAAGGAGGCAGAGGCAGTCTGTGTTACAGAAGAAGCCCAAGGCTGAGGACTTAAGAACTGAGT
  GTTCAACTTGCAAAGGAACTGGAGATAATGAGAATCTTGTCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211047 representing BC040236
  Red=Cloning site Green=Tags(s)
  
  MDRSSKRRQVKPLAASLLEALDYDSSDDSDFKVGDASDSEGSGNGSEDPSKDSGEGSCSDSEENILEEEL
  NEDIQVKEEQLKNSTEEIMPSDKQLIKMEKKEEEENGERPRKKKEKEKEKEKEREKDKEKATISDSAAAS
  AAGTTPATSPPAVTSPSVPTTTTTTTEEQVSEPKKWNLRRNRPLLDFVSMEELNAMDDYDSEDDNDWRPT
  VVKRKGRSASQKEGSDGDNEDDDDEGSGSEEDENDEGNDEDHSSPASEAGGKKKRSKVLSRNSADDEELT
  NDSLTLSQSKSNEDSLILEKSQNWSSQKMDHILICCVCLGDNSEDADEIIQCDNCGITVHEGCYGVDGES
  DSIMSSASENSTEPWFCDACKCGVSPSCELCPNQDGIFKETDAGRWVHIVCALYVPGVAFGDIDKLRPVT
  LTEMNYSKYGAKECSFCEDPRFARTGVCISCDAGMCRAYFHVTCAQKEGLLSEAAAEEDIADPFFAYCKQ
  HADRLDRKWKRKNYLALQSYCKMSLQEREKQLSPEAQARINARLQQYRAKAELARSTRPQAWVPREKLPR
  PLTSSASAIRKLMRKAELMGISTDIFPVDNSDTSSSVDGRRKHKQPALTADFVNYYFERNMRMIQIQENM
  AEQKNIKDKLENEQEKLHVEYNKLCESLEELQNLNGKLRSEGQGIWALLGRITGQKLNVPAILRAPKERK
  PSKKEGGTQKTSALPTVLYSCGICKKNHDQHLLLLCDTCKLHYHLGCLDPPLTRMPRKTKNSYWQCSECD
  QAGSSDMEAEMAMETLPDGTKRSRRQIKEPVKFVPQDVPPEPKKIPIRNTRTRGRKRSFVPEEEKHEERV
  PRERRQRQSVLQKKPKAEDLRTECSTCKGTGDNENLVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC040236
• ORF Size: 2634 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC040236.1
• RefSeq Size: 3998 bp
• RefSeq ORF: 2636 bp
• Locus ID: 75725
• Cytogenetics: 6 A1
• MW: 146.5 kDa