Epas1 (NM_010137) Mouse Tagged ORF Clone
CAT#: MR211029
- TrueORF®
Epas1 (Myc-DDK-tagged) - Mouse endothelial PAS domain protein 1 (Epas1)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_010137" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Epas1 |
Synonyms | bHLHe73; HIF-2alpha; HIF2A; HLF; HRF; MOP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211029 representing NM_010137
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCTGACAAGGAGAAAAAAAGGAGCAGCTCAGAGCTGAGGAAGGAGAAATCCCGTGATGCCGCGA GGTGCCGGCGCAGCAAGGAGACGGAGGTCTTCTATGAGTTGGCTCATGAGTTGCCCCTGCCTCACAGTGT GAGCTCCCACCTGGACAAAGCCTCCATCATGCGCCTGGCCATCAGCTTCCTTCGGACACATAAGCTCCTG TCCTCAGTCTGCTCTGAAAATGAATCTGAAGCTGAGGCCGACCAGCAAATGGATAACTTGTACCTGAAAG CCTTGGAGGGTTTCATTGCTGTGGTGACCCAAGACGGTGACATGATCTTTCTGTCGGAAAACATCAGCAA GTTCATGGGACTTACTCAGGTAGAACTAACAGGACACAGCATCTTTGACTTCACTCATCCTTGCGACCAT GAGGAGATCCGTGAGAACCTGACTCTCAAAAACGGCTCTGGTTTTGGGAAGAAGAGCAAAGACGTGTCCA CCGAGCGTGACTTCTTCATGAGGATGAAGTGCACGGTCACCAACAGAGGCCGGACTGTCAACCTCAAGTC GGCCACCTGGAAGGTCCTGCACTGCACCGGGCAAGTGAGAGTCTACAACAACTGCCCCCCTCACAGTAGC CTCTGTGGCTCCAAGGAGCCCCTGCTGTCCTGCCTTATCATCATGTGTGAGCCAATCCAGCACCCATCCC ACATGGACATCCCCCTGGACAGCAAGACTTTCCTGAGCCGCCACAGCATGGACATGAAGTTCACCTACTG TGACGACAGAATCTTGGAACTGATTGGTTACCACCCCGAGGAGCTACTTGGACGCTCTGCCTATGAGTTC TACCATGCCCTGGATTCGGAGAACATGACCAAAAGTCACCAGAACTTGTGCACCAAGGGGCAGGTGGTAT CTGGCCAGTACCGGATGCTAGCCAAACACGGAGGATATGTGTGGCTGGAGACCCAGGGGACGGTCATCTA CAACCCCCGCAACCTGCAGCCTCAGTGTATCATGTGTGTCAACTATGTGCTGAGTGAGATCGAGAAGAAC GACGTGGTGTTCTCCATGGACCAGACCGAATCCCTGTTCAAGCCACACCTGATGGCCATGAACAGCATCT TTGACAGCAGTGACGATGTGGCTGTAACTGAGAAGAGCAACTACCTGTTCACCAAACTGAAGGAGGAGCC CGAGGAACTGGCCCAGTTGGCCCCCACCCCAGGAGATGCCATTATTTCTCTCGATTTCGGAAGCCAGAAC TTCGATGAACCCTCAGCCTATGGCAAGGCCATCCTTCCCCCGGGCCAGCCATGGGTCTCGGGGCTGAGGA GCCACAGTGCCCAGAGCGAGTCCGGGAGCCTGCCAGCCTTCACTGTGCCCCAGGCAGACACCCCAGGGAA CACTACACCCAGTGCTTCAAGCAGCAGTAGCTGCTCCACGCCCAGCAGCCCTGAGGACTACTATTCATCC TTGGAGAATCCCTTGAAGATCGAAGTGATTGAGAAGCTTTTCGCCATGGACACGGAGCCGAGGGACCCGG GCAGTACCCAGACGGACTTCAGTGAACTGGATTTGGAGACCTTGGCACCCTACATCCCTATGGACGGCGA GGACTTCCAGCTGAGCCCCATCTGCCCAGAGGAGCCGCTCATGCCAGAGAGCCCCCAGCCCACCCCCCAG CACTGCTTCAGTACCATGACCAGCATCTTCCAGCCGCTCACCCCGGGGGCCACCCACGGCCCCTTCTTCC TCGATAAGTACCCGCAGCAGTTGGAAAGCAGGAAGACAGAGTCTGAGCACTGGCCCATGTCTTCCATCTT CTTTGATGCTGGGAGCAAAGGGTCCCTGTCTCCATGCTGTGGCCAGGCCAGCACCCCTCTCTCTTCTATG GGAGGCAGATCCAACACGCAGTGGCCCCCGGATCCACCATTACATTTCGGCCCTACTAAGTGGCCTGTGG GTGATCAGAGTGCTGAATCCCTGGGAGCCCTGCCGGTGGGGTCATCGCAGTTGGAACCTCCGAGCGCCCC GCCTCATGTCTCCATGTTCAAGATGAGGTCTGCAAAGGACTTCGGGGCCCGAGGTCCATACATGATGAGC CCAGCCATGATCGCCCTGTCCAACAAGCTGAAGCTAAAGCGGCAGCTGGAGTATGAGGAGCAAGCCTTCC AAGACACAAGCGGGGGGGACCCTCCAGGCACCAGCAGTTCACACTTGATGTGGAAACGTATGAAGAGCCT CATGGGCGGGACCTGTCCTTTGATGCCTGACAAGACCATCAGTGCGAACATGGCCCCCGATGAATTCACC CAAAAATCTATGAGAGGCCTGGGCCAGCCACTGAGACACCTGCCACCTCCCCAGCCACCATCTACCAGGA GCTCAGGGGAGAACGCCAAGACTGGGTTCCCGCCACAGTGCTATGCCTCCCAGTTCCAGGACTACGGTCC TCCAGGAGCTCAAAAGGTGTCAGGCGTGGCCAGTCGACTGCTGGGGCCATCGTTCGAGCCTTACCTGTTG CCGGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGTGCCTGGAAGCTCCACACTCCTGCAGGGGA GAGACCTTCTCAGAGCTCTGGACCAGGCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211029 representing NM_010137
Red=Cloning site Green=Tags(s) MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLL SSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDH EEIRENLTLKNGSGFGKKSKDVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSS LCGSKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRSAYEF YHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKN DVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTKLKEEPEELAQLAPTPGDAIISLDFGSQN FDEPSAYGKAILPPGQPWVSGLRSHSAQSESGSLPAFTVPQADTPGNTTPSASSSSSCSTPSSPEDYYSS LENPLKIEVIEKLFAMDTEPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQ HCFSTMTSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQASTPLSSM GGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSMFKMRSAKDFGARGPYMMS PAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTISANMAPDEFT QKSMRGLGQPLRHLPPPQPPSTRSSGENAKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLL PELTRYDCEVNVPVPGSSTLLQGRDLLRALDQAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010137 |
ORF Size | 2622 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010137.3, NP_034267.3 |
RefSeq Size | 5352 bp |
RefSeq ORF | 2625 bp |
Locus ID | 13819 |
UniProt ID | P97481 |
Cytogenetics | 17 E4 |
MW | 97.2 kDa |
Gene Summary | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222307 | Epas1 (untagged) - Mouse endothelial PAS domain protein 1 (Epas1), (10ug) |
USD 881.00 |
|
MR211029L3 | Lenti ORF clone of Epas1 (Myc-DDK-tagged) - Mouse endothelial PAS domain protein 1 (Epas1) |
USD 1,100.00 |
|
MR211029L4 | Lenti ORF clone of Epas1 (mGFP-tagged) - Mouse endothelial PAS domain protein 1 (Epas1) |
USD 1,100.00 |
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