Map7d1 (NM_144941) Mouse Tagged ORF Clone

CAT#: MR210935

Map7d1 (Myc-DDK-tagged) - Mouse microtubule-associated protein 7 domain containing 1 (Mtap7d1), transcript variant 1

Product Images

Circular map for MR210935

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map7d1
• Synonyms: AV028413; BC019977; mKIAA1187; Mtap7d1; Parcc1; Rprc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210935 representing NM_144941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTGGCCCGCGTGTGGAGCCCGGCCCGGGCGCACCCGCAGCTGTGTTAGCCAGGATCCCCCAAG
  AGCCAAGACCGTCTCCGGAAGGTGACCCTTCCCCTCCTCCGCCGCCAACACCGATGTCAGCCTTGGTTCC
  TGACACTCCCCCAGACACACCTCCTGCCCTGAAGACTGCCACGAATCCTAAGCAGCTCCCACTGGAACCA
  GGGAATCCCACAGGGCAGATCTCACCTCAGCCAGCTCCCCCACAGGAAGAATGCCCGTCCTCAGAAGCCA
  AGAGCAGGGGCCCCACCCCCACAGCCACAGGACCACGGGAGGCCAAGCCTTCTCGAAGAAGCAGCCAACC
  ATCCCCCACCACAGTGCCAGCCTCTGACAGCCCTCCTGCCAAGCAAGATGTAAAGAAGGCAGGAGAGAGA
  CACAAGCTTGCCAAAGAGCGCCGAGAAGAGCGGGCCAAGTACCTGGCGGCCAAGAAGGCAGTGTGGTTGG
  AGAAGGAGGAAAAAGCCAAGGCGCTCCGGGAAAAGCAACTCCAGGAGCGCCGCCGACGGCTGGAGGAGCA
  GCGGCTTAAAGCCGAACAGCGCCGGGCTGCCCTGGAGGAGCGGCAGAGGCAGAAGCTTGAGAAAAACAAG
  GAGCGCTATGAAGCAGCAATCCAGCGGTCAGTGAAGAAGACGTGGGCTGAGATCCGGCAACAGCGCTGGT
  CCTGGGCAGGGGCCCTGCACCACAGCTCCCCTGGACGTAAGACCAGTGGGAGCAGGTGCTCCGTGTCGGC
  AGTAAACCTGCCCAAACACGTGGACTCTATAATCAACAAGCGGCTCTCAAAGTCCTCTGCCACGCTCTGG
  AACTCCCCCAGTAGAAATCGCAGCCTGCAGCTGAGCGCATGGGAGAGCAGCATTGTGGATCGTCTGATGA
  CGCCCACCCTCTCCTTCCTGGCTCGGAGTCGCAGCGCGGTCACACTGCCCCGCAACGGCAGGGACCAGGG
  TAGGGGCAGCGGCCCTGGGAGACGCCCCACGAGGGCCCGGGCAGGGGCCAGCCTCGCGCCCGGGCCACAC
  CCCGACCGCACTCATCCCTCTGCAGCCGTGCCAGTGTGCCCGCGCTCGGCCTCCGCCAGCCCCCTGACGC
  CTTGTAGCGCCCCTCGGAGCGCGCACCGCTGCACCCCGTCCGGGGAGCGCCCAGAGCGGCGCAAGCCCGG
  CGCCGGTGGCAGCCCCGCGCTGGCGCGCCGCCGGCTGGAAGCCACGCCGGTGCAGAAAAAGGAGAAGAAG
  GACAAGGAGCGGGAAAACGAGAAGGAAAAGAGCGCCCTGGCCCGCGAACGGAACCTCAAGAAGCGCCAGT
  CGCTGCCAGCGTCTATCAGACCTCGGCTCTCCACCGGCTCTGAGCTCAGCCCTAAGTCCAAGGCCCGGCC
  ATCCTCTCCCTCTACAACCTGGCACAGACCTGCCTCCCCCTGCCCCAGCCCAGGACCAGGACATGCCCTT
  CCTCCAAAACCACCATCCCCCCGAGGCACCACTGCATCACCCAAGGGGCGGGTTCGGAGGAAGGAGGAGG
  CAAAAGAGAGCCCCAGCCCGTCGGGGCCTGAGGACAAAAACCACCGCAAGAGTAGAGCCGCTGAGGAGAA
  GGAGCCAGCTGCCCCAGCCTCACCAGCGCCCTCACCGGTGCCCTCGCCCACCCCAGCCCAGCCTCAGAAG
  GAGCAATCCTCAACACAGATCCCTGCAGAAACTGCTGTCCCGGCTGTCCCAGCTGCTCCGACTGCCCCTC
  CTACCGCTGCTCCCTCGGTGACCCCTAGCAAACCCATGGCGGGCACCACAGACCGAGAGGAAGCCACGCG
  GCTCCTGGCTGAAAAGCGGCGCCAGGCTCGGGAACAGAGGGAACGCGAAGAACAGGAGCGGAAGCTGCAG
  GCTGAAAGGGACAAGCGAATGCGAGAGGAGCAGCTGGCCCGGGAGGCTGAAGCCCGGGCTGAACGTGAGG
  CCGAGGCGCGGAGACGGGAGGAGCAGGAGGCTCGAGAGAAGGCGCAGGCTGAGCAGGAGGAGCAGGAGCG
  ACTGCAGAAGCAGAAAGAGGAAGCCGAAGCCCGGTCCCGGGAAGAAGCTGAGCGCCAGCGCCAGGAGCGG
  GAAAAGCACTTTCAGAAGGAGGAACAGGAGAGACAAGAGCGAAGAAAGCGGCTGGAGGAGATAATGAAGA
  GGACTCGGAAATCAGAAGCCGCCGAAACCAAGAAGCAGGATGCAAAGGAGACCGCAGCTAACAATTCCGG
  CCCAGACCCTGTGAAAGCTGTAGAGACTCGGCCCTCTGGGCTACAGAAGGATTCTATGCAGAAAGAGGAG
  CTGGCCCCCCAGGAGCCACAGTGGAGCCTGCCAAGCAAGGAGATGCCAGGGTCCCTGGTAAATGGCCTGC
  AGCCTCTCCCAGCGCACCAGGAGAACGGCTTCTCCCCAAAGGGAACCGCTGGGGACAAGAGTCTGGGTCG
  CACAGCAGAGGGGCTCCTCCCATTTGCAGAGGCAGAAGCCTTCCTCAAGAAAGCAGTGGTGCAGCCTCCA
  CAGGTCACAGAAGTTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210935 representing NM_144941
  Red=Cloning site Green=Tags(s)
  
  MESGPRVEPGPGAPAAVLARIPQEPRPSPEGDPSPPPPPTPMSALVPDTPPDTPPALKTATNPKQLPLEP
  GNPTGQISPQPAPPQEECPSSEAKSRGPTPTATGPREAKPSRRSSQPSPTTVPASDSPPAKQDVKKAGER
  HKLAKERREERAKYLAAKKAVWLEKEEKAKALREKQLQERRRRLEEQRLKAEQRRAALEERQRQKLEKNK
  ERYEAAIQRSVKKTWAEIRQQRWSWAGALHHSSPGRKTSGSRCSVSAVNLPKHVDSIINKRLSKSSATLW
  NSPSRNRSLQLSAWESSIVDRLMTPTLSFLARSRSAVTLPRNGRDQGRGSGPGRRPTRARAGASLAPGPH
  PDRTHPSAAVPVCPRSASASPLTPCSAPRSAHRCTPSGERPERRKPGAGGSPALARRRLEATPVQKKEKK
  DKERENEKEKSALARERNLKKRQSLPASIRPRLSTGSELSPKSKARPSSPSTTWHRPASPCPSPGPGHAL
  PPKPPSPRGTTASPKGRVRRKEEAKESPSPSGPEDKNHRKSRAAEEKEPAAPASPAPSPVPSPTPAQPQK
  EQSSTQIPAETAVPAVPAAPTAPPTAAPSVTPSKPMAGTTDREEATRLLAEKRRQAREQREREEQERKLQ
  AERDKRMREEQLAREAEARAEREAEARRREEQEAREKAQAEQEEQERLQKQKEEAEARSREEAERQRQER
  EKHFQKEEQERQERRKRLEEIMKRTRKSEAAETKKQDAKETAANNSGPDPVKAVETRPSGLQKDSMQKEE
  LAPQEPQWSLPSKEMPGSLVNGLQPLPAHQENGFSPKGTAGDKSLGRTAEGLLPFAEAEAFLKKAVVQPP
  QVTEVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144941
• ORF Size: 2538 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144941.3, NP_659190.3
• RefSeq Size: 3402 bp
• RefSeq ORF: 2541 bp
• Locus ID: 245877
• UniProt ID: A2AJI0
• Cytogenetics: 4 D2.2
• MW: 93.3 kDa