Dpp10 (NM_199021) Mouse Tagged ORF Clone

CAT#: MR210711

Dpp10 (Myc-DDK-tagged) - Mouse dipeptidylpeptidase 10 (Dpp10)

Product Images

Circular map for MR210711

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dpp10
• Synonyms: 6430601K09Rik; DPP X; Dprp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210711 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCCATGAAGCAGGAGCAGCAACCCACCCCAGGGGCCAGGGCAACCCAGTCGCAGCCAGCCGACC
  AGGAACTAGGAAGTAACAGCCCTCCACAAAGAAACTGGAAGGGAATCGCCATTGCCCTGCTGGTGATCTT
  GGTGGTATGCTCACTCATCACAATGTCTGTCATCCTGTTAACACCAGATGAACTAACAAATTCTTCAGAA
  ACAAGACTGTCACTAGAGGAGCTTTTGGGGAAAGGATTCGGACTTCATAATCCAGAACCTCGGTGGATCA
  ATGATACAGTCGTGGTATATAAAACCAACAATGGACACGTCATGAAACTGAATACAGAATCAAATGCTTC
  CACATTGTTATTGGACAACTCAACTTTTGTAACGTTTAAAGCCTCCAGACACTCACTTTCCCCAGATTTA
  AAGTATGTTCTCCTGGCATACGACGTTAAGCAGATTTTTCATTACTCATTTACCGCTTCATATTTGATTT
  ACAACATACACACTGGGGAAGTGTGGGAGTTGAATCCCCCAGAAGTTGAGGACTCAGTGTTGCAGTATGC
  GGCCTGGGGTGTGCAAGGACAGCAGCTGATTTATATTTTTGAAAACAATATCTACTATCAACCTGATATC
  AAGAGCAGTTCTTTACGACTAACATCTTCAGGAAAGGAAGGCATTATTTTTAATGGGATTGCTGACTGGT
  TATATGAAGAAGAACTTCTGCATTCTCATATCGCCCACTGGTGGTCCCCCGATGGAGAGAGACTTGCCTT
  CCTGATGATAAATGACTCGTTGGTGCCTAACATGATCATACCTCGGTTTACTGGAGCACTGTACCCCAAA
  GCAAAGCAGTACCCATACCCCAAGGCAGGTCAAGCGAACCCATCAGTGAAGTTATATGTTGTAAACCTGT
  ATGGACCAACTCATACTTTGGAGCTGATGCCACCCGACATTTTTAAATCAAGAGAATATTACATCACCAT
  GGTTAAATGGGTGAGCAACACGAGGACAGTGGTCAGGTGGTTGAATCGGCCTCAGAACATCTCCATCCTC
  ACACTCTGTGAATCTACCACCGGGGCATGCAGCAGGAAATATGAGATGACGTCCGACACCTGGCTCTCTA
  AACAGAACGAGGAGCCAGTGTTTTCCAGAGATGGAAGCAAGTTCTTCATGACTGTTCCTGTTAAACAAGG
  TGGAAGAGGAGAATTCCACCACATAGCTATGTTCCTGGTCCAGAGTAAAAGCGAGCAAATTACGGTGCGG
  CATCTGACATCAGGAAACTGGGAAGTGATAAGGATCTTGGCCTATGATGAAACAACTCAAAAAATTTACT
  TTCTGAGCACAGAATCTTCTCCCCAAGGGAGGCAACTATACAGTGCTTCTACTGAGGGACTATTGAATCG
  TGATTGCATCTCATGCAACTTTATGAAAGAAGATTGCACGTATTTTGATGCCAGCTTTAGCCCCATGAAT
  CAACATTTCTTGTTATTCTGTGAAGGTCCAAAGGTCCCAGTGGTCAGCCTTCACATCACAGACAACCCAT
  CAAGGTATTTCCTCTTGGAAAACAATTCTGTGATGAAAGAAACTATTCAGAAGAAGAAGCTCGCAAAGAG
  AGAGACTAGAATACTTCACATTGATGACTATGAACTTCCTTTACAGTTGTCCTTTCCCAAAGATTTTATG
  GAGAAAAACCAGTATGCTCTTCTATTAATAATGGATGAAGAACCAGGAGGCCAAATGGTGACAGATAAGT
  TCCATGTTGACTGGGATTCAGTTCTTATTGACACCGATAATGTCATTGTAGCAAGATTTGATGGCAGAGG
  AAGTGGATTCCAGGGCCTGAAAGTTTTGCAGGAGATTCACAGAAGGATAGGCTCAGTGGAGGCAAAGGAC
  CAAGTAGCTGCTGTAAAATATTTACTGAAACAGCCATATATTGACTCCAAAAGATTAAGCATTTTTGGAA
  AGGGATATGGGGGCTATATTGCATCAATGATCTTAAAATCAGATGAGAAGTTTTTCAAATGTGGAGCCGT
  GGTTGCACCCATCTCAGACATGAAGTTGTATGCCTCAGCTTTCTCTGAACGGTACCTTGGCATGCCATCA
  AAGGAAGAAAGCACTTACCAGGCATCCAGTGTACTGCATAACATTCATGGTTTAAAAGAAGAAAATTTAT
  TAATAATTCACGGAACTGCTGATACAAAAGTTCATTTCCAGCATTCAGCAGAATTGATCAAGCATCTGAT
  AAAAGCTGGGGTGAATTACACTCTGCAGGTCTATCCAGATGAAGGATATCACATTTCAGACAAGAGCAAG
  CATCATTTTTACAGCACAATTCTCAGATTCTTCAGTGATTGTCTAAAGGAAGAGGTATCTGTGCTGCCAC
  AGGAACCAGAAGAAGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210711 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAMKQEQQPTPGARATQSQPADQELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELTNSSE
  TRLSLEELLGKGFGLHNPEPRWINDTVVVYKTNNGHVMKLNTESNASTLLLDNSTFVTFKASRHSLSPDL
  KYVLLAYDVKQIFHYSFTASYLIYNIHTGEVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDI
  KSSSLRLTSSGKEGIIFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPNMIIPRFTGALYPK
  AKQYPYPKAGQANPSVKLYVVNLYGPTHTLELMPPDIFKSREYYITMVKWVSNTRTVVRWLNRPQNISIL
  TLCESTTGACSRKYEMTSDTWLSKQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLVQSKSEQITVR
  HLTSGNWEVIRILAYDETTQKIYFLSTESSPQGRQLYSASTEGLLNRDCISCNFMKEDCTYFDASFSPMN
  QHFLLFCEGPKVPVVSLHITDNPSRYFLLENNSVMKETIQKKKLAKRETRILHIDDYELPLQLSFPKDFM
  EKNQYALLLIMDEEPGGQMVTDKFHVDWDSVLIDTDNVIVARFDGRGSGFQGLKVLQEIHRRIGSVEAKD
  QVAAVKYLLKQPYIDSKRLSIFGKGYGGYIASMILKSDEKFFKCGAVVAPISDMKLYASAFSERYLGMPS
  KEESTYQASSVLHNIHGLKEENLLIIHGTADTKVHFQHSAELIKHLIKAGVNYTLQVYPDEGYHISDKSK
  HHFYSTILRFFSDCLKEEVSVLPQEPEEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199021
• ORF Size: 2400 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199021.3, NP_950186.3
• RefSeq Size: 4648 bp
• RefSeq ORF: 2403 bp
• Locus ID: 269109
• UniProt ID: Q6NXK7
• Cytogenetics: 1 E2.3
• MW: 91.1 kDa
• Gene Summary: Promotes cell surface expression of the potassium channel KCND2 (PubMed:22311982). Modulates the activity and gating characteristics of the potassium channel KCND2 (PubMed:22311982). Has no dipeptidyl aminopeptidase activity (Probable).[UniProtKB/Swiss-Prot Function]