Qars (NM_133794) Mouse Tagged ORF Clone

CAT#: MR210578

Qars (Myc-DDK-tagged) - Mouse glutaminyl-tRNA synthetase (Qars), transcript variant 1

Product Images

Circular map for MR210578

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Qars
• Synonyms: 1110018N24Rik; 1200016L19Rik; C80286; GLNRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210578 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACTCCAGATTCGCTAGCGCTGTTCACCGGCCTTGGCCTTAGCGAGAACAAGGCCCGCGAGACGC
  TCAAGAACGAGGCTCTGAGCACTCAGCTGCGCGAGGCGGCGACCCAGGCACACCAGATTCTGGGTTCTAC
  CATCGACAAGGCTACTGGGGTCCTCCTATATGACTTGGTCTCCCGACTCAGGGATACTCGGCGTCGTTCT
  TTCCTTGTGAGCTATATAGCCAATAAGAAGATTCACACGGGACTCCAGCTGAGCGCTGCTCTTGAATATG
  TTCGGAGTCATCCCCAGGATCCCATTGATACCAAGGACTTCGAGCAGGAGTGTGGCGTCGGTGTGGTGGT
  GACACCGGAGCAGATTGAGGAAGCTGTGGAGTCCACCATAAATAAGCATCAGTTGCAGCTCCTAGCGGAA
  CGGTACCGTTTCAACATGGGGCTGCTAATGGGAGAGGCTCGGGCTGCGCTCAGATGGGCAGACGGCAAAA
  TGATCAAGAACGAGGTGGATATGCAGGTGCTTCACCTTTTGGGGCCCAAGATGGAAGCTGATCTGGTGAA
  GAAGCCCAAGGTGGCAAAGGCACGGCTGGAAGAAACAGACCGGAAGACAGCAAAAGATGTGGTGGAGAAA
  GGTGAAGTGGCTGGCCAGACCCTGTCTCTGATGGAGCAGCTCCGGGGGGAGGCACTTAAGTTTCATAAGC
  CAGGTGAGAACTACAAGACGCCAGGCTATGTGATCACTCCATATACCATGGATCTGCTGAAGCAGCACCT
  GGAGATCACTGGGGGACAGGTACGTACGCGGTTCCCTCCAGAGCCCAATGGAATCCTGCATATTGGACAC
  GCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAGGCCAACAATGGTATCTGTTTTCTGCGCTTTGATG
  ACACCAACCCTGAGAAAGAAGAAGCAAAATTCTTCACTGCTATTTATGACATGGTGACCTGGCTGGGTTA
  TACACCTTACAAAGTGACATATGCTTCTGACTATTTTGACCAGCTGTATGCCTGGGCCGTGGAACTCATC
  CATGGGGGTCTTGCTTATGTGTGTCACCAGAGAGTGGAAGAGCTCAAAGGCCATAACCCTTTACCTTCTC
  CATGGAGGGACAGGCCTAAGGAAGAATCATTGCTGCTCTTTGAGGCAATGCGCAAGGGCAAATTTGCAGA
  AGGAGAGGCCACACTTCGAATGAAGTTGGTGATGGAAGATGGCAAGATGGACCCCGTGGCCTATCGAGTC
  AAGTACACGCCACACCATCGCACAGGGGACAAATGGTGCATCTACCCCACCTATGACTACACACATTGTC
  TGTGTGACTCCATTGAGCACATTACCCACTCGTTGTGTACCAAGGAGTTCCAGGCTCGACGGTCTTCCTA
  CTTTTGGTTATGTAACGCTCTGAAAGTCTATTGTCCTGTTCAGTGGGAATATGGTCGCCTCAATTTGCAC
  TATGCTGTTGTCTCAAAGCGCAAGATTCTCCAGCTTGTAGCAGCTGGTGCTGTTCGGGACTGGGACGATC
  CACGGCTCTTCACACTCACTGCCCTACGACGACGGGGTTTTCCACCAGAGGCTATCAACAACTTCTGTGC
  TCGGGTTGGGGTCACAGTGGCACAGACCACAATGGAACCTCATCTTCTGGAAGCCTGTGTGCGTGATGTG
  CTGAATGATGCAGCCCCACGTGCCATGGCTGTTCTAGAGCCACTACAAGTTGTCATCACCAACTTTCCTG
  CTCCCAAGCCCTTGGACATCCGAGTGCCAAATTTCCCAGCTGATGAGACCAAGGGTTTCCACCAGGTTCC
  TTTTGCTTCCACTGTCTTCATTGAGAGAAGCGACTTTAAGGAGGAGTCAGAACCAGGCTATAAGCGCCTA
  GCTTCGGGCCAGCCTGTGGGCCTGAGACACACTGGTTATGTCATTGAACTGCAGAATATTGTCAGGGGCT
  CCAGTGGCTGTGTGGAACGCTTGGAGGTGACCTGTAGACGAGCTGATGCTGGAGAGAAGCCCAAGGCCTT
  TATTCACTGGGTATCACAGCCTTTGGTGTGTGAGATTCGCCTCTATGAGTGCCTATTCCAGCACAAGAAC
  CCCGAAGACCCTGTTGAAGTGCCTGGTGGATTCCTAAGTGACCTGAACCCGGCGTCACTACAAGTGGTAG
  AAGGAGCATTAGTGGACTGCTCTGTGGCTTTGGCAAAGCCCTTTGACAAGTTCCAGTTTGAGCGCCTTGG
  GTACTTCTCTGTGGATCCAGACAGCCATCAAGGACAGATTGTCTTCAACCGAACTGTCACACTAAAAGAA
  GACCCAGGCAAAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210578 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATPDSLALFTGLGLSENKARETLKNEALSTQLREAATQAHQILGSTIDKATGVLLYDLVSRLRDTRRRS
  FLVSYIANKKIHTGLQLSAALEYVRSHPQDPIDTKDFEQECGVGVVVTPEQIEEAVESTINKHQLQLLAE
  RYRFNMGLLMGEARAALRWADGKMIKNEVDMQVLHLLGPKMEADLVKKPKVAKARLEETDRKTAKDVVEK
  GEVAGQTLSLMEQLRGEALKFHKPGENYKTPGYVITPYTMDLLKQHLEITGGQVRTRFPPEPNGILHIGH
  AKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAIYDMVTWLGYTPYKVTYASDYFDQLYAWAVELI
  HGGLAYVCHQRVEELKGHNPLPSPWRDRPKEESLLLFEAMRKGKFAEGEATLRMKLVMEDGKMDPVAYRV
  KYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALKVYCPVQWEYGRLNLH
  YAVVSKRKILQLVAAGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDV
  LNDAAPRAMAVLEPLQVVITNFPAPKPLDIRVPNFPADETKGFHQVPFASTVFIERSDFKEESEPGYKRL
  ASGQPVGLRHTGYVIELQNIVRGSSGCVERLEVTCRRADAGEKPKAFIHWVSQPLVCEIRLYECLFQHKN
  PEDPVEVPGGFLSDLNPASLQVVEGALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGQIVFNRTVTLKE
  DPGKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133794
• ORF Size: 2328 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133794.2
• RefSeq Size: 2501 bp
• RefSeq ORF: 2328 bp
• Locus ID: 97541
• UniProt ID: Q8BML9
• Cytogenetics: 9 F2
• MW: 87.7 kDa
• Gene Summary: Glutamine--tRNA ligase. Plays a critical role in brain development.[UniProtKB/Swiss-Prot Function]