Htatsf1 (NM_028242) Mouse Tagged ORF Clone

CAT#: MR210497

Htatsf1 (Myc-DDK-tagged) - Mouse HIV TAT specific factor 1 (Htatsf1), transcript variant 1

Product Images

Circular map for MR210497

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Htatsf1
• Synonyms: 1600023H17Rik; 2600017A12Rik; 2700077B20Rik; TAT-SF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210497 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCAACAACTTGAGCGGGAACGATGAGTTTGATGAGCAATTGCGAATGCAAGAATTGTATGGAG
  GAGACCCCAAGGAAGGTGACACCCAGAATGAGCCCTCTGGAGAAGCCCATTCTTTGGGACAACCACCAGA
  TGACACTCCTTACGAGTGGGACCTTGATAAGAAGGCTTGGTTCCCTAAGATCACCGAAGATTTCATTGCT
  ACGTATCAGGCTAACTACGGCTTTTCTAGTGATGGTGCATCCAGCTCTACTGCGAACGTCCAGGACGCCA
  ACACTAAGGCTGTAGAAGAACCTCCACAAAAAGAAGTCCCGGAGACCCCTGACTCCAAAAGGAAGGGGGA
  AAAGAGAAAGGCTGAATCTGGATGGTTCCATGTTGAAGAAGACAGAAATACAAATGTATACGTGTCAGGT
  CTCCCTCCAGACATCACAGTGGATGAATTTATTCAGCTCATGTCCAAATTTGGCATTATTATGAGAGATC
  CTCAGACTGAAGAGTTTAAAGTTAAACTGTACAAAGATGATCAAGGGAATCTTAAAGGAGATGGGCTTTG
  TTGTTATCTGAAGAAAGAATCTGTGGAGCTTGCATTAAAACTTTTGGATGAAGATGAAATTAGAGGCTAC
  AAATTGCATGTGGAGGTGGCAAAGTTCCAGCTCAAGGGTGAATACGATGCCTCAAAGAAGAAGAAGAAGT
  GCAAAGATTATAAGAAGAAGCTGTCTCTACAACAGAAGCAGTTGGATTGGAGACCTGAGAGACGAGCTGG
  ACCAAACCGGCTGCGCCATGAGCGAGTTGTCATTCTCAAAAATATGTTTCACCCCATGGACTTTGAGGAT
  GACCCATTGGTACTGAATGAGATCAGAGAAGATCTGCGAGTAGAATGTTCCAAGTTTGGGCAGATTCGGA
  AGCTCCTTCTGTTTGATAGACACCCAGATGGTGTGGCCTCTGTGTCCTTCAGAGAGCCAGAGGAGGCTGA
  CCATTGTATTCAGACTCTGGATGGAAGGTGGTTTGGTGGCCGACAGATCACTGCTCAAGCCTGGGATGGG
  ACTACAGATTATCAGGTAGAGGAGACCTCAAGAGAAAGAGAGGAGCGGCTGAGAGGCTGGGAGGCATTCC
  TCAATGCTCCTGAGGCCAGCAGAGGCCTCCGGCGGATGGATTCCATCGCTGGTTCAGAAAGGCCAGGGCC
  TTCCAGAATGAGGCATTTTTCAGAGCACCCAAGTATGTCTAATATGAAGGCTCAGGAAGCTACAACTGGA
  ATGGCATTTGAAGAAACTATCGATGAAAATAAGTTTGAAAAGGCAGAAGAAGGGGGAGAATCCGAAGGAG
  ATGCTTCTGAAAAAGATGCCAAAGAAGGTGGGTCTGATGGGGACCACCCTGAGAGAGAGGGTGGAGAAGG
  CTGCTCCAAGAAAGAGAATGAAGAGGGCTGCCCTGAGAGGGCGCTTGAGCCTGAGGAGGGTAATCCTCAA
  ACCGAGGCTCAAGAGAATGGCCCTGAAAGGGAAGCTAGGAAGAAAAGCAAAATGGATTATGAGAAGAATG
  GCTTTTCAAAAGAGTCTGAAGACAATGACCTTGGCAAGGAGTCTGAAGGGGAAGACAGCCTCAAGAAAGA
  GTCGGAAGATGATGATTCAGAGGAGGAATCTGAGGAAGACAGCTCAGAAAAGCAATCTCAAGATGGCTCT
  GACAAAGAAATAGAAGAAAATGGTGTTAAAAAAGATGTTGACCAGGATGTCTCTGACAAGGAGTTCCCAG
  AAGATGTTGAGAAAGAGTCAGAAGAAAATGAGACTGACAAATCAGAATTTGATGAAGGTTCTGAAAGAGT
  GTTAGATGAGGAAGGCTCGGAGAGAGAATTTGAGGAAGACTCAGATGAAAAGGAGGAAGAAGGTGATGAT
  GATGAAGAAGAGGTCGTATATGAAAGGGTTTTTGATGATGATTCTGATGACATTGAGGAGGAAGAAGAAG
  CAGATGAAAAGGAGTGTGAAGATGCTGATGACAAAGAGGAAGACAATGATATAGATGAAAAGCTCTTTGA
  TGACTCCGATGAGAAGGAAGATGAAGAAGACACAGATGGAAAGAAAGATGATGATGCCAGTGACAAGGTA
  TTCGAAGACAATTCCAATGAGAAGTTGTTTGATGAGGAAGAAGGTCCCAATGAGAAGTTGTTTGATGATT
  CTGATGAGAGAGGGACTGTGGGGAATGTGAAGGAAGATGGGTCTCAGTCCACAGACAGCAGCTTTGCCCT
  CAGTAGTAGTGATGATGATGATGATGAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210497 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGNNLSGNDEFDEQLRMQELYGGDPKEGDTQNEPSGEAHSLGQPPDDTPYEWDLDKKAWFPKITEDFIA
  TYQANYGFSSDGASSSTANVQDANTKAVEEPPQKEVPETPDSKRKGEKRKAESGWFHVEEDRNTNVYVSG
  LPPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDDQGNLKGDGLCCYLKKESVELALKLLDEDEIRGY
  KLHVEVAKFQLKGEYDASKKKKKCKDYKKKLSLQQKQLDWRPERRAGPNRLRHERVVILKNMFHPMDFED
  DPLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFREPEEADHCIQTLDGRWFGGRQITAQAWDG
  TTDYQVEETSREREERLRGWEAFLNAPEASRGLRRMDSIAGSERPGPSRMRHFSEHPSMSNMKAQEATTG
  MAFEETIDENKFEKAEEGGESEGDASEKDAKEGGSDGDHPEREGGEGCSKKENEEGCPERALEPEEGNPQ
  TEAQENGPEREARKKSKMDYEKNGFSKESEDNDLGKESEGEDSLKKESEDDDSEEESEEDSSEKQSQDGS
  DKEIEENGVKKDVDQDVSDKEFPEDVEKESEENETDKSEFDEGSERVLDEEGSEREFEEDSDEKEEEGDD
  DEEEVVYERVFDDDSDDIEEEEEADEKECEDADDKEEDNDIDEKLFDDSDEKEDEEDTDGKKDDDASDKV
  FEDNSNEKLFDEEEGPNEKLFDDSDERGTVGNVKEDGSQSTDSSFALSSSDDDDDEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028242
• ORF Size: 2274 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028242.2
• RefSeq Size: 2855 bp
• RefSeq ORF: 2274 bp
• Locus ID: 72459
• UniProt ID: Q8BGC0
• Cytogenetics: X A6
• MW: 86.2 kDa
• Gene Summary: Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation (By similarity).[UniProtKB/Swiss-Prot Function]