Trim36 (NM_178872) Mouse Tagged ORF Clone

CAT#: MR210329

Trim36 (Myc-DDK-tagged) - Mouse tripartite motif-containing 36 (Trim36), transcript variant 1

Product Images

Circular map for MR210329

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim36
• Synonyms: D18Wsu100e; haprin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210329 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAATCAGAGGAGATAAGTGAATTTGGCTACATTATGGAATTGCTTGCCAAGGGCAAGGTGACTA
  TTAAGAATATCGAAAAGGAGCTTATTTGCCCAGCGTGCAAGGAGCTGTTTACCCACCCGCTGATCCTTCC
  CTGCCAGCACAGCGTCTGTCATAAGTGTGTGAAAGAACTTTTACTGTCTCTTGATGACTCGTTCAATGAT
  GTGGCGTCAGACAGCTCAAATCAGAGCAGCCCTAGGCTCCGGCTCACCTCCCCTAGCATGGATAAAATCG
  ACAAGATTAACAGACCAGGCTGGAAGCGTAATTCACTGACCCCGAGACCAACCACGTTCCCTTGTCCTGG
  CTGTGAACATGATGTGGATCTTGGAGAGCGAGGAGTCAGTGGTCTGTTTCGAAATTTCACTTTGGAAACG
  ATTGTCGAGAGATACCGGCAGGCCGCTAGGGCAGCCACAGCCATTATGTGTGACCTTTGTAAACCTCCAC
  CCCAGGAATCTACAAAGAGCTGCATGGATTGTAGTGCAAGCTACTGCAATGAATGCTTCAAAATTTATCA
  TCCCTGGGGCACTGTAAAAGCCCAGCATGAGTATGTGGGCCCCACCACTAATTTCAGACCCAAGGTTCTA
  ATGTGCCCAGAACATGAGACAGAGAGAATAAACATGTACTGTGAACTATGCAGAAGGCCAGTTTGCCACC
  TCTGTAAGTTGGGTGGGAATCATTCCAATCACCGGGTAACCACCATGAGCAGTGCCTACAAAACCTTAAA
  GGAGAAACTTTCAAAAGACATTGATTTCCTTATTGGCAAGGAAAGCCAGGTGAAGAGTCAAATTTCTGAA
  CTAAACTTGCTAATGAAAGAGACAGAGTGCAACGGAGAGAGGGCGAAGGAAGAAGCGCTGGCGCATTTTG
  AAAAGCTCTTTGAAATCCTGGAAGACAGGAAGTCGTCTGTTCTGAAAGCCATAGATGCCTCTAAGAAACT
  AAGACTAGACAAGTTTCACACTCAGATGGAAGAGTACCAAGGCCTTCTAGAGAATAACGGGCTCGTGGGG
  TATGCTCAGGAAGTGCTGAAGGAGACGGATCAGTCTTGCTTTGTGCAGACGGCGAAGCAGCTCCATCTCA
  GAATACAGAAAGCTACGGAGTCCCTGAAGAGCTTTAGACCTGCAGCCCAGGCTTCTTTTGAAGACTATGT
  TGTTAACATATCGAAACAAACAGAGGTGCTTGGAGAGTTGTCCTTTTTCTCCAGTGGCATAGACATTCCT
  GAGATCAACGAGGAACAGAGTAAAGTGTATAATAACGCCTTGATAGACTGGCATCATCCAGAAAAGGACA
  AAGCCGACAGCTATGTTCTAGAATACCGCAAGATTAATAGAGACGAAGAAATGATATCATGGAATGAGAT
  AGAAGTTCACGGCACAAGTAAAGTTGTCTCCAACCTTGAAAGCAACAGTCCCTATGCGTTCCGAGTGAGA
  GCTTACAGGGGTTCTATCTGCAGTCCCTGCAGCAGAGAATTGATCCTGCATACTCCTCCAGCTCCAGTTT
  TCAGTTTCCTGTTCGATGAGAAGTGTGGCTACAACACTGAGCACCTCTTGCTGAACCTGAAGAGAGACCG
  GGTGGAGAGCAGAGCTGGATTTAACGTCCTCCTGGCTGCGGAGCGCATCCAAGTGGGCCATTACACAAGC
  TTAGACTACATCATCGGGGATGTCGGAGTCACGAAAGGTAAACACTTCTGGGCCTGCCGCGTGGAACCGT
  ATTCATACCTGGTGAAAGTGGGAGTTGCTTCCAGCGACAAACTGCAAGAGTGGCTGCGCTCTCCCCGAGA
  TGCAGCTAGTCCAAGATATGAGCAAGACAGTGGACATGACAGTGGAAGCGAGGACGCCTGTTTTGATTCT
  TCACAGCCCTTTACATTAGTTACTATAGGCATGAAGAAATTTTTTATACCCAAGTCACCTACTTCCTCTA
  ATGAACCAGAAAACAGAGTTCTCCCCATGCCAACGAGTATAGGGATTTTCCTTGACTGTGATAAAGGCAA
  AGTGAGCTTCTATGATATGGACCACATGAAATGCCTGTATGAGCGCCAGGTGGACTGTTCGCATACAATG
  TATCCGGCCTTTGCCTTGATGGGCAGCGGAGGAATTCAGCTTGAGGAAGCCATCACAGCAAAGTATCTGG
  AATATGAAGAGGATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210329 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSESEEISEFGYIMELLAKGKVTIKNIEKELICPACKELFTHPLILPCQHSVCHKCVKELLLSLDDSFND
  VASDSSNQSSPRLRLTSPSMDKIDKINRPGWKRNSLTPRPTTFPCPGCEHDVDLGERGVSGLFRNFTLET
  IVERYRQAARAATAIMCDLCKPPPQESTKSCMDCSASYCNECFKIYHPWGTVKAQHEYVGPTTNFRPKVL
  MCPEHETERINMYCELCRRPVCHLCKLGGNHSNHRVTTMSSAYKTLKEKLSKDIDFLIGKESQVKSQISE
  LNLLMKETECNGERAKEEALAHFEKLFEILEDRKSSVLKAIDASKKLRLDKFHTQMEEYQGLLENNGLVG
  YAQEVLKETDQSCFVQTAKQLHLRIQKATESLKSFRPAAQASFEDYVVNISKQTEVLGELSFFSSGIDIP
  EINEEQSKVYNNALIDWHHPEKDKADSYVLEYRKINRDEEMISWNEIEVHGTSKVVSNLESNSPYAFRVR
  AYRGSICSPCSRELILHTPPAPVFSFLFDEKCGYNTEHLLLNLKRDRVESRAGFNVLLAAERIQVGHYTS
  LDYIIGDVGVTKGKHFWACRVEPYSYLVKVGVASSDKLQEWLRSPRDAASPRYEQDSGHDSGSEDACFDS
  SQPFTLVTIGMKKFFIPKSPTSSNEPENRVLPMPTSIGIFLDCDKGKVSFYDMDHMKCLYERQVDCSHTM
  YPAFALMGSGGIQLEEAITAKYLEYEEDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178872
• ORF Size: 2190 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178872.4, NP_849203.2
• RefSeq Size: 4556 bp
• RefSeq ORF: 2190 bp
• Locus ID: 28105
• UniProt ID: Q80WG7
• Cytogenetics: 18 24.41 cM
• MW: 82.8 kDa
• Gene Summary: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:19232519). Involved in chromosome segregation and cell cycle regulation (PubMed:19232519). May play a role in the acrosome reaction and fertilization (PubMed:12917430).[UniProtKB/Swiss-Prot Function]