Agbl5 (BC057349) Mouse Tagged ORF Clone

CAT#: MR210236

Agbl5 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901)

Product Images

Circular map for MR210236

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agbl5
• Synonyms: 4930455N08; 9430057O19Rik; CCP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210236 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATGAACAAGCAAAGCAAACTGTATTCCCAGGGCATGGCCCCCTTTGTGCGCACACTGCCTTCCC
  GGCCACGCTGGGAACGCATTAGAGAGCGGCCCACCTTTGAGATGACAGAGACACAGTTTGTGCTATCCTT
  TGTTCACCGTTTCGTAGAGGGCCGGGGAGCCACCACCTTCTTCGCCTTCTGCTACCCCTTCTCCTACAGT
  GACTGCCAGGATCTGCTGAGCCAGCTAGACCAGCGCTTTTCAGAGAATTACTCTACCCATAGCAGTCCTC
  TGGATAGTATTTATTACCACCGAGAGCTTCTCTGCTATTCTCTGGATGGACTTCGTGTAGATCTACTAAC
  AATCACTTCCTGCCATGGGCTTCGAGATGATCGGGAGCCCCGACTAGAGCAGCTATTTCCTGATCTCGGC
  ACTCCCCGACCATTCCGTTTCACAGGCAAAAGGATATTCTTCTTAAGCAGTAGGGTACACCCTGGAGAGA
  CTCCATCTAGCTTTGTGTTCAATGGCTTTCTGGACTTCATCCTTCGACCTGATGATCCCCGGGCACAAAC
  TCTCCGTCGACTCTTTGTTTTTAAGTTGATTCCCATGTTAAACCCTGATGGTGTGGTCCGGGGCCACTAC
  CGCACGGACTCCCGTGGAGTGAATCTGAACCGTCAGTACTTGAAGCCTGATGCCGTCCTGCACCCGGCCA
  TCTATGGCGCCAAAGCTGTGCTGCTTTATCATCACGTGCACTCGAGGCTCAACGCCAAGAGTCCCACGAA
  CCAGCAACCCACTCTCCATCTCCCTCCGGAAGCCCCTCTCTCTGACCTCGAGAAAGCCAATAATCTCCAC
  AATGAAGCTCACCTCGGGCAGTCACCTGATGGGGAAAACCCTGCGACTTGGCCTGAGACAGAGCCAGCAG
  AAGAGAAGACTGACCCTGTGTGGCTTATGCCACAGCCAATTCCTGAACTCGAGGAGCCAGCCCCTGACAC
  CATCCCCCCAAAAGAGAGTGGTGTTGCTTACTATGTAGACTTGCACGGACATGCTTCCAAAAGGGGCTGC
  TTCATGTATGGCAACAGCTTTAGTGACGAGAGCACCCAGGTGGAAAACATGCTATATCCAAAGCTCATCT
  CCTTGAATTCAGCCCACTTTGACTTCCAGGGCTGCAATTTCTCAGAGAAGAACATGTATGCCCGAGACCG
  TAGAGATGGCCAGTCCAAAGAGGGAAGCGGCCGTGTCGCAATCTACAAAGCCTCAGGGATAATCCACAGC
  TACACACTTGAATGCAACTACAACACTGGACGCTCAGTCAACAGCATCCCTGCTGCCTGCCATGACAACG
  GGCGCGCCAGCCCCCCTCCCCCTCCGGCTTTCCCCTCCAGATACACTGTGGAACTATTCGAGCAGGTGGG
  GCGAGCTATGGCCATCGCAGCTCTGGACATGGCAGAGTGTAATCCGTGGCCCCGAATTGTGCTGTCGGAA
  CACAGCAGCCTCACCAACCTGCGAGCCTGGATGCTGAGGCACGTGCGCAACAGCAGAGGGCTGACCAGTG
  CTGGGAACATGGGCGCGAGCAAGAAGCGAGGGGCTCGAACCCCTCCCAAAAGCAACAACAGCTTGCCTGT
  TTCCTGCTCTGAAAATGCTCTGAGCCGGGTTCGCAGTTTTAGCACCGGCACGAGTACTGGTGGCAGCAGC
  AGCAGCCAACAAAACTCTCCACAGATGAAGAACTCTCCCAGCTTCCCTTTTCATGGCAGTCGGACTGCGG
  GGCTACCAGGCCTGGGCTCTAGCACCCAAAAGGTCAGCCATCGGGTGCTGGGCCCTGTCAGAGAGCCCCG
  ATGCTCAGACAGAAGGCGGCGACAGCAGCCACTGAACCATCGCTCTACCACAAGCAGCCTGGCCCCATCC
  CCAACTCTGGCTAGTTCTGGCCCAACCTCCTCACGAAACATGGGCTCCTGCCTCCTACCCAATTCACTCA
  GCTTATCAGGGAGCAGCTGCTCATTCTCGTCTTCAGGGGACAAGCCAGAGGCTGTCATGGTGATTGGGAA
  AAGCCTACTAGGGGCTGGTGCTCGGATCCCTTGCATTAGGACTCGATTGCAGACTCCATTGTCCCTGCCA
  GATGCACCCCAACCACAGCCTACTAGCTTTGGCTTTCCTACCCGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210236 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNMNKQSKLYSQGMAPFVRTLPSRPRWERIRERPTFEMTETQFVLSFVHRFVEGRGATTFFAFCYPFSYS
  DCQDLLSQLDQRFSENYSTHSSPLDSIYYHRELLCYSLDGLRVDLLTITSCHGLRDDREPRLEQLFPDLG
  TPRPFRFTGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHY
  RTDSRGVNLNRQYLKPDAVLHPAIYGAKAVLLYHHVHSRLNAKSPTNQQPTLHLPPEAPLSDLEKANNLH
  NEAHLGQSPDGENPATWPETEPAEEKTDPVWLMPQPIPELEEPAPDTIPPKESGVAYYVDLHGHASKRGC
  FMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHS
  YTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSE
  HSSLTNLRAWMLRHVRNSRGLTSAGNMGASKKRGARTPPKSNNSLPVSCSENALSRVRSFSTGTSTGGSS
  SSQQNSPQMKNSPSFPFHGSRTAGLPGLGSSTQKVSHRVLGPVREPRCSDRRRRQQPLNHRSTTSSLAPS
  PTLASSGPTSSRNMGSCLLPNSLSLSGSSCSFSSSGDKPEAVMVIGKSLLGAGARIPCIRTRLQTPLSLP
  DAPQPQPTSFGFPTRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057349
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057349, AAH57349
• RefSeq Size: 3332 bp
• RefSeq ORF: 2150 bp
• Locus ID: 231093
• Cytogenetics: 5 B1
• MW: 79.5 kDa
• Gene Summary: Metallocarboxypeptidase that mediates protein deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins (PubMed:20519502, PubMed:21074048). In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/CCP1, AGBL2/CCP2, AGBL3/CCP3, AGBL1/CCP4 and AGBL4/CCP6 (PubMed:25103237). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768).[UniProtKB/Swiss-Prot Function]