Ncl (NM_010880) Mouse Tagged ORF Clone
CAT#: MR210180
- TrueORF®
Ncl (Myc-DDK-tagged) - Mouse nucleolin (Ncl)
ORF Plasmid: tGFP
"NM_010880" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ncl |
Synonyms | B530004O11Rik; C23; D0Nds28; D1Nds28; Nucl |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210180 representing NM_010880
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGCTCGCAAAGGCTGGCAAAACCCACGGTGAGGCCAAGAAAATGGCTCCTCCTCCAAAGGAGG TGGAAGAGGATAGTGAAGATGAAGAAATGTCAGAAGATGAAGATGACAGCAGTGGAGAAGAGGAGGTTGT CATCCCTCAGAAAAAAGGCAAAAAGGCTACCACAACCCCAGCAAAGAAGGTGGTTGTTTCACAAACAAAA AAGGCTGCAGTTCCCACACCAGCTAAGAAAGCAGCTGTGACCCCAGGCAAAAAGGCAGTAGCCACACCAG CTAAGAAAAACATTACACCAGCCAAAGTCATTCCAACACCGGGTAAGAAGGGAGCTGCACAAGCAAAAGC GTTGGTACCAACTCCTGGTAAAAAGGGAGCTGCCACTCCAGCTAAGGGGGCTAAGAACGGTAAGAATGCC AAGAAGGAAGACAGTGATGAGGATGAAGATGAAGAGGATGAAGATGATAGCGATGAGGATGAAGATGATG AGGAAGAGGATGAGTTTGAGCCACCAATAGTAAAAGGAGTGAAGCCAGCAAAAGCAGCTCCTGCTGCTCC TGCCTCAGAGGATGAGGAAGATGATGAGGATGAAGATGATGAGGAAGATGATGATGAAGAGGAGGAAGAT GACTCTGAGGAAGAAGTTATGGAGATCACAACAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTA TGAAAGCCAAGAGTGTGGCTGAGGAGGAGGATGATGAGGAAGAGGATGAAGATGACGAGGATGAGGATGA TGAGGAAGAGGATGACGAAGATGATGATGAGGAAGAAGAGGAGGAAGAACCTGTTAAAGCAGCACCTGGA AAACGGAAGAAGGAGATGACCAAGCAGAAAGAAGCCCCTGAAGCCAAGAAACAGAAAGTAGAAGGCTCAG AACCAACTACACCTTTCAATCTGTTCATTGGAAACCTTAATCCAAACAAGTCTGTTAATGAATTAAAATT TGCCATCAGTGAACTTTTTGCTAAAAATGATCTTGCTGTTGTGGATGTCAGAACTGGTACAAATAGGAAA TTTGGTTATGTGGACTTTGAGTCTGCTGAAGACCTAGAAAAGGCCTTGGAGCTCACTGGTTTAAAAGTGT TTGGCAATGAAATTAAACTAGAAAAACCAAAAGGAAGAGATAGTAAGAAAGTTCGAGCTGCAAGAACACT TCTAGCCAAAAACCTCTCTTTCAACATCACTGAGGATGAATTAAAGGAAGTGTTTGAAGACGCCATGGAG ATCAGATTAGTCAGCCAGGATGGGAAAAGTAAAGGGATTGCTTATATTGAATTTAAGTCTGAAGCTGATG CAGAGAAAAATTTGGAAGAAAAGCAGGGGGCAGAAATTGATGGACGATCTGTTTCACTCTACTATACTGG AGAGAAAGGTCAAAGGCAAGAGAGAACTGGAAAGACCAGCACTTGGAGTGGTGAATCAAAGACTTTGGTT TTAAGTAACCTTTCCTACAGTGCAACAAAAGAAACTCTTGAGGAAGTATTTGAGAAAGCAACTTTTATCA AAGTGCCCCAGAACCCACATGGCAAACCTAAAGGGTATGCATTTATAGAATTTGCTTCATTTGAAGATGC TAAAGAAGCTTTAAATTCCTGTAATAAAATGGAAATTGAGGGCAGAACAATCAGGCTGGAGTTGCAAGGA TCCAATTCGAGAAGTCAACCATCCAAAACTCTGTTTGTCAAAGGTCTGTCTGAGGATACCACTGAAGAGA CCTTAAAAGAATCATTTGAGGGCTCTGTTCGTGCAAGAATAGTCACTGATCGGGAAACTGGTTCTTCCAA AGGGTTTGGTTTTGTAGACTTTAATAGTGAGGAAGATGCCAAAGCTGCCAAGGAGGCCATGGAAGATGGA GAAATTGACGGAAACAAAGTTACCTTGGACTGGGCCAAACCTAAGGGTGAAGGTGGCTTTGGTGGTCGAG GTGGAGGCAGAGGAGGTTTCGGAGGCAGAGGTGGAGGCAGAGGTGGAAGAGGTGGATTTGGAGGAAGAGG CCGGGGAGGCTTTGGAGGTAGAGGAGGCTTCCGAGGCGGCAGAGGAGGAGGGGGAGACTTCAAGCCACAA GGAAAGAAGACGAAGTTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210180 representing NM_010880
Red=Cloning site Green=Tags(s) MVKLAKAGKTHGEAKKMAPPPKEVEEDSEDEEMSEDEDDSSGEEEVVIPQKKGKKATTTPAKKVVVSQTK KAAVPTPAKKAAVTPGKKAVATPAKKNITPAKVIPTPGKKGAAQAKALVPTPGKKGAATPAKGAKNGKNA KKEDSDEDEDEEDEDDSDEDEDDEEEDEFEPPIVKGVKPAKAAPAAPASEDEEDDEDEDDEEDDDEEEED DSEEEVMEITTAKGKKTPAKVVPMKAKSVAEEEDDEEEDEDDEDEDDEEEDDEDDDEEEEEEEPVKAAPG KRKKEMTKQKEAPEAKKQKVEGSEPTTPFNLFIGNLNPNKSVNELKFAISELFAKNDLAVVDVRTGTNRK FGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGRDSKKVRAARTLLAKNLSFNITEDELKEVFEDAME IRLVSQDGKSKGIAYIEFKSEADAEKNLEEKQGAEIDGRSVSLYYTGEKGQRQERTGKTSTWSGESKTLV LSNLSYSATKETLEEVFEKATFIKVPQNPHGKPKGYAFIEFASFEDAKEALNSCNKMEIEGRTIRLELQG SNSRSQPSKTLFVKGLSEDTTEETLKESFEGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDG EIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDFKPQ GKKTKFE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010880 |
ORF Size | 2121 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010880.3, NP_035010.3 |
RefSeq Size | 8257 bp |
RefSeq ORF | 2124 bp |
Locus ID | 17975 |
UniProt ID | P09405 |
Cytogenetics | 1 43.94 cM |
MW | 76.7 kDa |
Gene Summary | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).[UniProtKB/Swiss-Prot Function] |
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