Shkbp1 (NM_138676) Mouse Tagged ORF Clone
CAT#: MR210149
- TrueORF®
Shkbp1 (Myc-DDK-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_138676" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Shkbp1 |
Synonyms | B930062H15Rik; Sb1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210149 representing NM_138676
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTCGCGACGACTGCAGTGGAGGGGGTCCCGAGTCGGGGAGCACCCGGGGAAGTGATTCATCTGA ACGTAGGGGGCAAGCGGTTCAGTACCTCTCGCCAGACGCTTACCTGGATCCCAGACTCCTTCTTTTCCAG TCTTTTGAGCGGACGCATCTCAACCCTGAAAGATGAGACCGGAGCTATCTTCATCGACCGGGACCCCACC GTCTTTGCCCCCATCCTTAATTTCCTGCGGACCAAAGAGTTAGATCCCAGGGGTGTCCATGGTTCCAGCC TCCTCCATGAAGCCCAATTCTACGGACTCACTCCACTGGTTCGTCGCCTGCAGGTGAGGGAAGAGTTGGA TCGATCTTCCTGCGGAAATGTCCTCTTCAATGGTTATCTACCTCCTCCAGTGTTCCCAGTGAAGCGGCGA AACCGGCACAGCCTGGTGGGGCCCCAGCAAATAGGGGGACGCCCAGCCCCTGTTCGACGGAGCAACACGA TGCCCCCCAACTTGGGTAATGCAGGGCTGCTGGGCCGAATGTTAGATGAGAGAGCACCTCCATCCCCATC AGGCCAACCAGAGGAGCCAGGGATGGTGCGCCTGGTGTGTGGTCACCACAACTGGATTGCTGTGGCATAT ACCCACTTTCTCGTCTGTTATAGGTTGAAGGAAGCCTCGGGCTGGCAGCTTGCATTCTCCAGCCCGCGCC TGGACTGGCCCATCGAGCGCCTGGCTCTTGCGGCTCGGGTGCTTGGCGGTGCTCCGGGTGAGCATGACAA GATGGTGGCTGCAGCTACTGGCAGCGAGATCCTTCTGTGGGCCCTACAAGCACAAGGTGGTGGCTCTGAG ATAGGTGTCTTCCACCTGGGTGTGCCAGTGGAGGCCTTGTTCTTTGTTGGGAATCAGCTCATTGCCACAA GTCACACAGGGCGCATCGGTGTCTGGAACGCTGTGACCAAGCACTGGCAGGTCCAGGAGGTCCAGCCTAT CACGAGCTATGATGCAGCCGGCTCCTTCCTCCTCCTGGGCTGCAGCAATGGCTCCATCTATTATGTAGAT GTGCAGAAATTCCCCCTGCGGATGAAGGACAACGATCTCCTTGTCAGTGAGCTCTACCGAGACCCAGCAG AGGATGGGGTCACCGCCCTCAGCGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATTGAGAT TGCCTATGGCACCAGCTCCGGGGTGGTGCGGGTCATTGTGCAGCACCCTGAGACGGTGGGCTCGGGACCC CAGCTCTTCCAGACCTTCAGTGTGCACCGCAGCCCGGTCACTAAGATCATGCTGTCAGAGAAGCATCTTA TCTCTGTGTGTGCCGACAACAACCATGTTCGGACGTGGTCTGTGACCCGCTTTCGAGGCATGATCTCCAC CCAGCCTGGTTCCACTCCGCTGGCATCCTTCAAGATCCTGGCGCTGGAATCTGTTGATGGGCTCGGTGGC TGCAGTGCTGGCAATGACATTGGTCCCTATGGTGAACGGGATGACCAGCAGGTGTTCATCCAGAAAGTGG TGCCAAATGCCAGTCAGCTATTTGTGCGTCTCTCTTCCACTGGGCAGCGGGTGTGTTCTGTGCGCTCGGT GGATGGGTCTCCCACGACTGCCTTCACAGTGTTGGAGTGTGAGGGCTCCAGGCGGCTGGGCTCCAGACCC CGACGTTACCTGCTCACTGGTCAGGCCAACGGCAGCCTGGCCATGTGGGACCTGACCACTGCCATGGATG GCCTGGGCCAAACTCCTGCAGGCGGCCTGACAGAAGAAGAGCTGATGGACCAACTGGAGCAATGTGAGCT GAGCCCATTGGCTTCCTCTCGGGGCTCTTTCCCCAGCCCCTCACCCAGGACCTCTCTTACCAGTCTCCAC TCAGCCTCCAGCAACACCTCCCTGTATGGCCCCAGAGGGAGCCCCAGCCCACCACAGGCAGAGGCCCGTC GCCGCGGGGCAGGAAGCTTCGTGGATCGGTGTCAGGAACTGGCAAGGGGTGCGCCAGAGCTCAGATGGCC GCCAACACCAGCCCCTCGACCCTCCACCAGTCTTGGGAATCCCCTGACAGCTCTCAAGAAAACTCTTAAC GAAACTTCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210149 representing NM_138676
Red=Cloning site Green=Tags(s) MAVATTAVEGVPSRGAPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDETGAIFIDRDPT VFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQVREELDRSSCGNVLFNGYLPPPVFPVKRR NRHSLVGPQQIGGRPAPVRRSNTMPPNLGNAGLLGRMLDERAPPSPSGQPEEPGMVRLVCGHHNWIAVAY THFLVCYRLKEASGWQLAFSSPRLDWPIERLALAARVLGGAPGEHDKMVAAATGSEILLWALQAQGGGSE IGVFHLGVPVEALFFVGNQLIATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCSNGSIYYVD VQKFPLRMKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGVVRVIVQHPETVGSGP QLFQTFSVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESVDGLGG CSAGNDIGPYGERDDQQVFIQKVVPNASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRP RRYLLTGQANGSLAMWDLTTAMDGLGQTPAGGLTEEELMDQLEQCELSPLASSRGSFPSPSPRTSLTSLH SASSNTSLYGPRGSPSPPQAEARRRGAGSFVDRCQELARGAPELRWPPTPAPRPSTSLGNPLTALKKTLN ETSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138676 |
ORF Size | 2112 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138676.2, NP_619617.2 |
RefSeq Size | 2347 bp |
RefSeq ORF | 2115 bp |
Locus ID | 192192 |
UniProt ID | Q6P7W2 |
Cytogenetics | 7 A3 |
MW | 76.6 kDa |
Gene Summary | Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation (PubMed:21830225).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203620 | Shkbp1 (untagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1), (10ug) |
USD 646.00 |
|
MG210149 | Shkbp1 (tGFP-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1) |
USD 845.00 |
|
MR210149L3 | Lenti ORF clone of Shkbp1 (Myc-DDK-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1) |
USD 945.00 |
|
MR210149L4 | Lenti ORF clone of Shkbp1 (mGFP-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1) |
USD 945.00 |
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