Slc28a3 (NM_022317) Mouse Tagged ORF Clone

CAT#: MR210144

Slc28a3 (Myc-DDK-tagged) - Mouse solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 (Slc28a3)

Product Images

Circular map for MR210144

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc28a3
• Synonyms: Cnt3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210144 representing NM_022317
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGGGCAGACCCGGGAAAGAATTCCGAGCCCAGTGAGAGCAAGATGAGCCTGGAGCTGAGGCCAA
  CAGCCCCCAGCGACCTGGGCCGCAGCAATGAAGCCTTTCAGGATGAAGACCTTGAACGGCAGAACACTCC
  AGGAAACAGCACAGTCAGGAACAGAGTTGTACAAAGCGGGGAACAAGGACACGCCAAACAGGACGACAGG
  CAGATCACGATTGAACAGGAGCCTCTAGGAAACAAAGAAGACCCAGAGGATGATAGCGAAGATGAGCATC
  AGAAAGGGTTCTTGGAGAGGAAGTATGATACAATTTGTGAGTTTTGTAGAAAGCACAGAGTTGTTCTCCG
  AAGCACCATCTGGGCCGTTTTACTGACAGGTTTCCTGGCCCTGGTGATTGCAGCCTGTGCGATAAACTTC
  CACAGAGCCCTTCCTCTTTTTGTGATCACCTTGGTGACCATCTTCTTTGTCATCTGGGATCACTTGATGG
  CCAAATATGAACAGCGAATAGATGATTTCCTGTCTCCTGGCAGAAGGCTTCTCGACAGGCATTGGTTCTG
  GCTGAAATGGGTGGTCTGGAGTTCATTGATCCTGGCGATTATTCTCTGGCTGTCTCTTGACACTGCCAAA
  CTGGGCCAACAAAACTTGGTATCCTTTGGCGGACTCATCATGTACCTTATCCTGTTATTTCTGTTTTCCA
  AACATCCCACCAGAGTTTACTGGAGGCCTGTCTTTTGGGGAATTGGACTACAGTTTCTTCTTGGGCTCTT
  AATTCTAAGAACTAGGCCTGGATTTGTTGCTTTTGATTGGATGGGGAGACAAGTTCAGACTTTCCTGGGG
  TACACTGATACTGGAGCCAGGTTTGTCTTTGGGGAAAAGTATACAGACCACTTCTTTGCATTTAAGATCC
  TGCCCATCGTGGTTTTCTTCAGCACCGTGATGTCTATGCTTTACTATCTGGGGCTGATGCAGTGGATCAT
  CAGAAAGGTCGGGTGGCTAATGCTTGTCACGATGGGGTCATCTCCCATTGAGTCTGTAGTTGCTGCTGGC
  AACATATTTATTGGGCAAACAGAGTCTCCACTACTGGTCCAACCATATTTACCACACGTCACCAAGTCGG
  AACTCCATACCATCATGACAGCTGGCTTTGCCACGATTGCTGGAAGTGTATTGGGCGCATATATCTCTTT
  TGGGGTGTCGTCCACGCATTTGCTAACTGCCTCGGTCATGTCCGCACCTGCAGCCCTGGCTGTGGCCAAA
  CTCTTTTGGCCTGAGACAGAGAAACCCAAAATAACCCTCAAGAGTGCCATGAAGATGGAAAATGGTGATT
  CAAGGAATCTCCTGGAGGCAGCCTCTCAGGGCGCATCCTCGTCCATCCCTCTGGTGGCAAACATTGCTGC
  GAATCTGATCGCCTTCCTGGCCTTGCTCTCCTTTGTGAACTCTGCCCTGTCTTGGTTTGGAAGCATGTTC
  AACTACCCAGAGCTGAGTTTTGAGCTCATTTGTTCCTACATCTTCATGCCCTTCTCTTTCATGATGGGAG
  TCGACTGGCAAGACAGCTTTATGGTCGCCAAACTCATAGGTTACAAGACATTCTTCAATGAATTTGTGGC
  TTATGACCACCTCTCAAAATTAATCAATTTGAGGAAAGCGGCTGGACCTAAATTTGTGAATGGCGTGCAG
  CAATATATGTCAATTCGCTCTGAGACCATCGCAACCTATGCCCTCTGTGGTTTTGCCAATTTTGGTTCCC
  TAGGAATCGTGATCGGTGGACTTACATCCATAGCACCATCCAGAAAACGAGACATCGCCTCTGGAGCCAT
  GAGAGCCCTGATTGCTGGCACCATTGCCTGCTTCATGACAGCCTGCATCGCAGGCATCCTCTCTGATACC
  CCGGTGGATATCAACTGCCATCACGTTCTAGAGAACGGCAGAGTACTCAGCAACACAACCGAAGTGGTGT
  CTTGTTGCCAAAATCTGTTCAACAGCACTGTTGCCAAGGGACCAAATGACGTCGTCCCAGGAGGAAACTT
  CAGCCTCTATGCTTTGAAGAGCTGCTGCAACCTGCTGAAACCACCAACGCTGAACTGCAATTGGATCCCT
  AATAAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210144 representing NM_022317
  Red=Cloning site Green=Tags(s)
  
  MSRADPGKNSEPSESKMSLELRPTAPSDLGRSNEAFQDEDLERQNTPGNSTVRNRVVQSGEQGHAKQDDR
  QITIEQEPLGNKEDPEDDSEDEHQKGFLERKYDTICEFCRKHRVVLRSTIWAVLLTGFLALVIAACAINF
  HRALPLFVITLVTIFFVIWDHLMAKYEQRIDDFLSPGRRLLDRHWFWLKWVVWSSLILAIILWLSLDTAK
  LGQQNLVSFGGLIMYLILLFLFSKHPTRVYWRPVFWGIGLQFLLGLLILRTRPGFVAFDWMGRQVQTFLG
  YTDTGARFVFGEKYTDHFFAFKILPIVVFFSTVMSMLYYLGLMQWIIRKVGWLMLVTMGSSPIESVVAAG
  NIFIGQTESPLLVQPYLPHVTKSELHTIMTAGFATIAGSVLGAYISFGVSSTHLLTASVMSAPAALAVAK
  LFWPETEKPKITLKSAMKMENGDSRNLLEAASQGASSSIPLVANIAANLIAFLALLSFVNSALSWFGSMF
  NYPELSFELICSYIFMPFSFMMGVDWQDSFMVAKLIGYKTFFNEFVAYDHLSKLINLRKAAGPKFVNGVQ
  QYMSIRSETIATYALCGFANFGSLGIVIGGLTSIAPSRKRDIASGAMRALIAGTIACFMTACIAGILSDT
  PVDINCHHVLENGRVLSNTTEVVSCCQNLFNSTVAKGPNDVVPGGNFSLYALKSCCNLLKPPTLNCNWIP
  NKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022317
• ORF Size: 2109 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022317.3, NP_071712.3
• RefSeq Size: 4565 bp
• RefSeq ORF: 2112 bp
• Locus ID: 114304
• UniProt ID: Q9ERH8
• Cytogenetics: 13 B1
• MW: 78.8 kDa
• Gene Summary: Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (By similarity).[UniProtKB/Swiss-Prot Function]