Stra6 (NM_001162475) Mouse Tagged ORF Clone

CAT#: MR209916

Stra6 (Myc-DDK-tagged) - Mouse stimulated by retinoic acid gene 6 (Stra6), transcript variant 3

Product Images

Circular map for MR209916

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stra6
• Synonyms: AI891933
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209916 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCCAGGCATCTGAGAATGGAAGCCAGACCTCCTCCGGGGTGACAGATGACTACAGCAGCTGGT
  ACATCGAGGAACCTCTAGGAGCTGAGGAGGTGCAGCCAGAGGGGGTGATTCCCCTTTGCCAGCTCACCGC
  ACCCCCTGCCCTGCTCCATGCCTGCCTGGCTTCACTGTCGTTCCTGGTTCTGCTGCTGCTGGCCTTGCTG
  GTGAGACGGCGCAGGCTTTGGCCACGCTGTGGTCATCGCGGACTTGGACTGCCCAGCCCTGTGGATTTCT
  TGGCTGGGGACCTATCCTGGACAGTGCCTGCTGCTGTCTTTGTGGTCCTCTTCAGCAACCTATGCCTGTT
  GCTCCCTGATGAGAACCCACTGCCTTTCCTGAACCTCACTGCAGCATCAAGTCCAGATGGAGAGATGGAG
  ACATCAAGAGGGCCCTGGAAGCTACTGGCTCTGCTCTACTATCCAGCCCTCTATTACCCTCTGGCTGCTT
  GTGCTTCGGCAGGGCACCAAGCTGCATTCCTATTGGGTACTGTGTTATCTTGGGCCCACTTTGGTGTTCA
  GGTCTGGCAGAAAGCTGAGTGTCCTCAGGATCCTAAGATCTACAAGCACTACTCCTTGCTGGCCTCCCTG
  CCTCTACTTCTGGGTCTTGGATTCCTGAGCCTTTGGTATCCAGTACAACTTGTGCAGAGTCTCCGTCACC
  CGACAGGAGCAGGCTCCCAGGGGCTGCAGACCAGCTACTCCGAGAAGTATCTGAGAACTCTTCTCTGCCC
  AAAGAAGTTGGATAGCTGCTCCCATCCTGCCTCCAAGCGTAGCCTCCTGTCTCGGGCCTGGGCCTTCTCC
  CATCATTCCATCTACACTCCACAGCCAGGATTCCGCCTGCCCTTGAAGCTGGTGATCTCGGCCACCCTGA
  CAGGAACAGCCACTTACCAGGTGGCCCTGCTGCTGCTGGTGAGCGTGGTGCCTACTGTGCAGAAGGTGAG
  GGCGGGGATCAACACAGATGTCTCCTACCTGCTGGCTGGCTTTGGGATCGTGCTCTCTGAAGACAGGCAG
  GAGGTGGTAGAGCTGGTGAAGCATCACCTATGGACTGTGGAAGCATGCTACATCTCAGCTCTGGTCTTGT
  CCTGCGCATCAACCTTCCTGCTCCTGATCCGATCCCTGAGGACACACAGGGCCAATCTTCAAGCACTACA
  CCGAGGGGCTGCCCTGGATCTGGACCCCCCTCTTCAGAGTATTCATCCCTCTCGCCAAGCCATAGTCAGC
  TGGATGAGCTTCTGTGCCTACCAGACGGCCTTCAGCTGCCTTGGGCTCCTGGTGCAGCAGGTCATCTTCT
  TCTTGGGGACCACATCCCTGGCCTTCCTGGTGTTTGTGCCTTTACTGCATGGCAGGAACCTCCTGCTGCT
  GCGATCCCTGGAATCCACGTGGCCCTTCTGGCTGACTGTGGCCTTAGCTGTAATCCTGCAGAACATAGCA
  GCCAACTGGATCTTCCTGAGGACTCACCATGGATACCCAGAGCTGACCAACCGGCGCATGCTCTGCGTAG
  CTACTTTTCTCCTCTTCCCCATCAACATGCTGGTGGGAGCCATAATGGCTGTCTGGCGGGTGCTCATCTC
  TTCTCTCTACAACACTGTTCACCTCGGCCAGATGGACCTCAGCCTGCTGCCGCAGAGGGCAGCCTCCCTG
  GATCCAGGCTACCACACATACCAAAACTTCCTGAGGATTGAGGCCAGCCAGTCACATCCAGGAGTCATAG
  CCTTCTGTGCCCTGCTCCTCCATGCTCCAAGTCCACAGCCCCGGCCCCCATTGGCCCCTCAGGACAGCCT
  CAGGCCGGCAGAAGAAGAAGAAGGGATGCAGTTGCTACAGACCAAGGACCTGATGGCCAAGGGAGCAGGA
  CACAAAGGCAGCCAGAGCAGGGCCCGCTGGGGTCTGGCCTACACATTGCTCCACAATCCAAGCCTACAGG
  CCTTCCGGAAGGCAGCCCTTACTAGTGCCAAGGCCAATGGCACCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209916 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESQASENGSQTSSGVTDDYSSWYIEEPLGAEEVQPEGVIPLCQLTAPPALLHACLASLSFLVLLLLALL
  VRRRRLWPRCGHRGLGLPSPVDFLAGDLSWTVPAAVFVVLFSNLCLLLPDENPLPFLNLTAASSPDGEME
  TSRGPWKLLALLYYPALYYPLAACASAGHQAAFLLGTVLSWAHFGVQVWQKAECPQDPKIYKHYSLLASL
  PLLLGLGFLSLWYPVQLVQSLRHPTGAGSQGLQTSYSEKYLRTLLCPKKLDSCSHPASKRSLLSRAWAFS
  HHSIYTPQPGFRLPLKLVISATLTGTATYQVALLLLVSVVPTVQKVRAGINTDVSYLLAGFGIVLSEDRQ
  EVVELVKHHLWTVEACYISALVLSCASTFLLLIRSLRTHRANLQALHRGAALDLDPPLQSIHPSRQAIVS
  WMSFCAYQTAFSCLGLLVQQVIFFLGTTSLAFLVFVPLLHGRNLLLLRSLESTWPFWLTVALAVILQNIA
  ANWIFLRTHHGYPELTNRRMLCVATFLLFPINMLVGAIMAVWRVLISSLYNTVHLGQMDLSLLPQRAASL
  DPGYHTYQNFLRIEASQSHPGVIAFCALLLHAPSPQPRPPLAPQDSLRPAEEEEGMQLLQTKDLMAKGAG
  HKGSQSRARWGLAYTLLHNPSLQAFRKAALTSAKANGTQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162475
• ORF Size: 2013 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162475.1, NP_001155947.1
• RefSeq Size: 2784 bp
• RefSeq ORF: 2013 bp
• Locus ID: 20897
• UniProt ID: O70491
• Cytogenetics: 9 B
• MW: 73.7 kDa
• Gene Summary: Functions as retinol transporter (PubMed:23839944, PubMed:24852372). Accepts all-trans retinol from the extracellular retinol-binding protein RBP4, facilitates retinol transport across the cell membrane, and then transfers retinol to the cytoplasmic retinol-binding protein RBP1. Retinol uptake is enhanced by LRAT, an enzyme that converts retinol to all-trans retinyl esters, the storage forms of vitamin A (By similarity). Contributes to the activation of a signaling cascade that depends on retinol transport and LRAT-dependent generation of retinol metabolites that then trigger activation of JAK2 and its target STAT5, and ultimately increase the expression of SOCS3 and inhibit cellular responses to insulin (PubMed:21368206, PubMed:23839944). Important for the homeostasis of vitamin A and its derivatives, such as retinoic acid and 11-cis-retinal (PubMed:22467576, PubMed:24852372). STRA6-mediated transport is particularly important in the eye, and under conditions of dietary vitamin A deficiency (PubMed:22467576, PubMed:23839944, PubMed:24852372). Does not transport retinoic acid (By similarity).[UniProtKB/Swiss-Prot Function]