Hspa2 (NM_008301) Mouse Tagged ORF Clone

CAT#: MR209622

Hspa2 (Myc-DDK-tagged) - Mouse heat shock protein 2 (Hspa2), transcript variant 1

Product Images

Circular map for MR209622

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hspa2
• Synonyms: 70kDa; Hsp70-2; HSP70.2; HSP70A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209622 representing NM_008301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGTCTGCCCGCGGCCCGGCTATCGGCATCGACCTGGGCACCACTTACTCGTGCGTGGGGGTATTCCAAC
  ATGGCAAGGTGGAGATCATCGCCAACGACCAGGGTAACCGCACCACCCCCAGCTACGTGGCCTTCACTGA
  CACCGAGCGTCTCATCGGCGACGCCGCCAAGAACCAAGTGGCCATGAACCCCACAAACACCATCTTCGAC
  GCCAAGCGGCTGATCGGACGGAAGTTCGAGGACGCCACAGTGCAGTCCGATATGAAGCACTGGCCGTTCC
  GAGTGGTGAGCGAAGGTGGGAAGCCCAAAGTGCAGGTGGAATATAAAGGGGAGATGAAGACCTTCTTCCC
  TGAGGAGATTTCTTCCATGGTCCTCACTAAGATGAAGGAGATCGCCGAAGCCTACCTGGGGGGCAAGGTG
  CAGAGCGCAGTCATCACTGTTCCTGCCTATTTCAACGACTCGCAGCGCCAGGCCACCAAGGATGCGGGCA
  CCATCACCGGCCTCAACGTGTTGCGCATCATCAATGAGCCCACAGCAGCGGCCATCGCCTACGGCCTGGA
  TAAGAAGGGCTGTGCGGGCGGCGAGAAGAACGTGCTCATCTTTGACCTGGGCGGGGGCACCTTCGATGTG
  TCCATCCTGACCATCGAGGATGGCATCTTTGAGGTGAAGTCCACGGCCGGCGATACCCACCTGGGTGGCG
  AAGACTTCGACAACCGTATGGTCAGCCACCTGGCGGAGGAGTTCAAGCGCAAACACAAGAAGGACATTGG
  GCCCAACAAGCGCGCTGTGCGCCGGCTGCGCACCGCCTGCGAGCGCGCTAAGCGCACCCTGAGCTCGTCC
  ACGCAGGCCAGCATAGAGATCGACTCGCTCTACGAGGGCGTGGATTTCTACACGTCCATCACCCGCGCCC
  GCTTCGAGGAGCTCAACGCCGATCTCTTCCGAGGGACCCTGGAGCCGGTGGAAAAGGCGCTGCGCGATGC
  CAAGCTAGACAAGGGCCAGATCCAGGAGATAGTGCTGGTGGGCGGCTCAACCCGCATCCCTAAGATCCAG
  AAGCTCCTGCAAGATTTCTTCAACGGCAAGGAGCTGAACAAGAGCATTAATCCCGACGAGGCGGTGGCCT
  ACGGCGCCGCTGTGCAGGCGGCTATCCTCATCGGCGACAAGTCGGAAAATGTGCAGGATCTGCTGTTACT
  CGACGTGACTCCATTGTCGCTCGGCATCGAAACAGCTGGCGGTGTCATGACCCCACTCATCAAGAGAAAC
  ACCACGATCCCCACCAAGCAGACGCAGACCTTCACTACCTACTCAGACAACCAGAGCAGCGTGCTGGTGC
  AAGTGTACGAGGGCGAACGGGCCATGACCAAGGACAATAACCTCTTGGGCAAGTTCGACCTGACTGGGAT
  CCCCCCAGCACCCCGTGGGGTCCCCCAGATCGAGGTCACCTTTGACATCGATGCCAACGGCATCCTTAAC
  GTCACTGCTGCCGACAAGAGCACCGGTAAAGAAAATAAAATCACCATAACCAACGACAAGGGTCGGCTGA
  GCAAAGACGACATTGACCGGATGGTGCAGGAGGCGGAGCGGTACAAATCGGAAGATGAAGCAAATCGCGA
  TCGCGTGGCAGCCAAAAATGCGGTGGAGTCCTATACCTACAACATCAAGCAGACCGTGGAAGACGAGAAA
  CTGAGGGGCAAGATTAGCGAGCAGGACAAAAACAAGATCCTCGACAAGTGTCAGGAGGTGATCAACTGGC
  TTGACCGAAACCAGATGGCAGAGAAAGATGAGTACGAACACAAGCAGAAAGAGCTTGAGAGAGTGTGCAA
  CCCCATCATCAGCAAACTTTACCAAGGCGGTCCAGGCGGCGGCGGCTCCTCTGGAGGGCCCACCATCGAG
  GAAGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209622 representing NM_008301
  Red=Cloning site Green=Tags(s)
  
  MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFD
  AKRLIGRKFEDATVQSDMKHWPFRVVSEGGKPKVQVEYKGEMKTFFPEEISSMVLTKMKEIAEAYLGGKV
  QSAVITVPAYFNDSQRQATKDAGTITGLNVLRIINEPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDV
  SILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLAEEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSS
  TQASIEIDSLYEGVDFYTSITRARFEELNADLFRGTLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQ
  KLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIGDKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRN
  TTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKDNNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILN
  VTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNAVESYTYNIKQTVEDEK
  LRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGGSSGGPTIE
  EVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008301
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008301.4, NP_032327.2
• RefSeq Size: 2595 bp
• RefSeq ORF: 1902 bp
• Locus ID: 15512
• UniProt ID: P17156
• Cytogenetics: 12 33.73 cM
• MW: 70.1 kDa
• Gene Summary: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (By similarity). Plays a role in spermatogenesis (PubMed:24557841). In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells (PubMed:24557841).[UniProtKB/Swiss-Prot Function]