Dclk3 (BC056929) Mouse Tagged Lenti ORF Clone
CAT#: MR209474L4
- LentiORF®
Lenti ORF clone of Dclk3 (mGFP-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292)
"BC056929" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Dclk3 |
Synonyms | BC056929; C730036H08; Dcamkl3 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR209474).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC056929 |
ORF Size | 1857 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC056929, AAH56929 |
RefSeq Size | 2692 bp |
RefSeq ORF | 1859 bp |
Locus ID | 245038 |
Cytogenetics | 9 F3 |
Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218040 | Dclk3 (untagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292), (10ug) |
USD 635.00 |
|
MG209474 | Dclk3 (tGFP-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292) |
USD 776.00 |
|
MR209474 | Dclk3 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292) |
USD 576.00 |
|
MR209474L3 | Lenti ORF clone of Dclk3 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292) |
USD 876.00 |
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