Lta4h (NM_008517) Mouse Tagged ORF Clone

CAT#: MR209403

Lta4h (Myc-DDK-tagged) - Mouse leukotriene A4 hydrolase (Lta4h)

Product Images

Circular map for MR209403

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lta4h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209403 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGAGGTCGCGGATACTTGCTCCTTGGCTTCTCCAGCCTCGGTCTGCCGAACCCAGCACCTCCATC
  TTCGCTGCAGCGTCGACTTCGCTCGCCGGACGCTGACCGGGACGGCCGCGCTCACGGTCCAGTCACAGGA
  GGAGAATCTGCGCAGCCTGACTTTGGACACAAAAGACCTCACAATAGAAAAAGTGGTGATCAATGGACAA
  GAAGTCAAATACACCCTTGGAGAAAGTCAAGGTTACAAAGGGTCACCGATGGAAATCTCTCTTCCCATCG
  CCCTGAGCAAAAATCAAGAGATCGTTATAGAAATTTCCTTTGAGACATCTCCCAAATCCTCTGCGCTTCA
  GTGGCTCACTCCTGAACAGACGTCAGGGAAGCAGCACCCGTATCTCTTCAGTCAGTGCCAGGCCATCCAC
  TGCAGGGCCATCCTCCCTTGCCAAGACACTCCTTCCGTGAAATTAACATACACCGCAGAGGTGTCTGTCC
  CGAAAGAACTGGTGGCTCTCATGAGTGCCATCCGTGATGGAGAAGCCCCTGACCCAGAAGACCCCAGCAG
  GAAGATTTACAGATTCAACCAGAGGGTTCCCATACCATGCTACCTGATTGCTTTGGTTGTTGGAGCTTTA
  GAAAGCAGACAAATTGGCCCAAGAACTCTGGTGTGGTCCGAGAAAGAGCAGGTGGAGAAATCTGCTAATG
  AATTTTCTGAGACCGAATCCATGCTTAAAATTGCAGAAGATCTGGGGGGGCCCTATGTTTGGGGGCAGTA
  CGACCTCTTGGTTCTGCCCCCATCCTTCCCGTACGGTGGCATGGAGAACCCTTGTCTCACGTTCGTAACG
  CCTACGCTACTGGCAGGTGACAAGTCACTCTCCAACGTTATTGCACATGAAATATCTCATAGCTGGACAG
  GAAACCTAGTGACTAACAAGACGTGGGATCACTTTTGGTTAAACGAAGGACATACTGTCTACTTAGAGCG
  CCACATTTGTGGACGGTTGTTTGGGGAAAAGTTCAGACATTTCCATGCTTTGGGAGGATGGGGAGAGCTA
  CAGAATACGATAAAAACATTTGGGGAGTCCCACCCCTTCACCAAGCTCGTGGTTGATCTGAAGGACGTAG
  ACCCCGATGTAGCCTATTCCTCCATCCCCTATGAGAAGGGCTTTGCACTACTCTTCTACCTTGAACAGCT
  TCTTGGGGGACCAGAGGTGTTTCTAGGATTCTTAAAGGCTTATGTTAAGAAGTTTTCCTACCAAAGTGTA
  ACGACTGATGACTGGAAGAGCTTCCTATATTCCCACTTTAAAGACAAGGTGGATCTTCTCAATCAAGTTG
  ATTGGAACGCCTGGCTCTACGCTCCGGGCCTGCCTCCTGTCAAGCCCAATTACGACGTGACTCTGACAAA
  TGCTTGCATTGCCTTAAGTCAAAGATGGGTTACTGCCAAAGAGGAAGATTTAAGCTCATTCAGCATCGCA
  GACCTGAAGGATCTCTCGTCCCATCAGCTGAATGAGTTCCTGGCACAGGTGCTTCAGAAGGCACCTCTTC
  CCTTGGGGCACATAAAGCGAATGCAAGAGGTGTACAACTTCAATGCCATTAACAATTCTGAAATACGATT
  CAGATGGTTACGGCTCTGCATTCAATCGAAATGGGAGGAAGCAATTCCTTTGGCCCTAAAGATGGCAACT
  GAACAAGGAAGGATGAAATTCACACGTCCTCTATTCAAGGATCTCGCTGCCTTTGACAAATCCCATGATC
  AAGCTGTCCACACCTACCAAGAGCATAAAGCCAGCATGCATCCCGTGACTGCGATGCTGGTGGGGAGAGA
  TCTGAAAGTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209403 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPEVADTCSLASPASVCRTQHLHLRCSVDFARRTLTGTAALTVQSQEENLRSLTLDTKDLTIEKVVINGQ
  EVKYTLGESQGYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKQHPYLFSQCQAIH
  CRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGEAPDPEDPSRKIYRFNQRVPIPCYLIALVVGAL
  ESRQIGPRTLVWSEKEQVEKSANEFSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVT
  PTLLAGDKSLSNVIAHEISHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFHALGGWGEL
  QNTIKTFGESHPFTKLVVDLKDVDPDVAYSSIPYEKGFALLFYLEQLLGGPEVFLGFLKAYVKKFSYQSV
  TTDDWKSFLYSHFKDKVDLLNQVDWNAWLYAPGLPPVKPNYDVTLTNACIALSQRWVTAKEEDLSSFSIA
  DLKDLSSHQLNEFLAQVLQKAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWEEAIPLALKMAT
  EQGRMKFTRPLFKDLAAFDKSHDQAVHTYQEHKASMHPVTAMLVGRDLKVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008517
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008517.1, NM_008517.2, NP_032543.2
• RefSeq Size: 2066 bp
• RefSeq ORF: 1836 bp
• Locus ID: 16993
• UniProt ID: P24527
• Cytogenetics: 10 C2
• MW: 69 kDa
• Gene Summary: The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]