Abce1 (NM_015751) Mouse Tagged ORF Clone

CAT#: MR209296

Abce1 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family E (OABP), member 1 (Abce1)

Product Images

Circular map for MR209296

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abce1
• Synonyms: C79080; Oabp; RLI; Rnaseli; RNS4I; RNS41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209296 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACAAATTAACAAGAATTGCTATTGTCAACCACGACAAATGCAAACCTAAGAAATGTCGACAGG
  AGTGCAAAAAGAGTTGCCCTGTGGTTCGGATGGGAAAATTGTGCATAGAAGTTACACCCCAGAGCAAAAT
  AGCATGGATTTCTGAAACTCTCTGTATTGGTTGTGGTATTTGTATTAAGAAATGTCCCTTTGGCGCCTTA
  TCAATTGTCAATTTGCCAAGCAACTTGGAAAAAGAAACAACACATCGCTACTGTGCCAATGCCTTCAAGC
  TTCACAGGTTGCCTATCCCTCGTCCAGGTGAAGTTTTGGGATTAGTTGGAACTAATGGTATTGGAAAGTC
  AACTGCACTAAAAATTTTAGCAGGAAAACAAAAGCCAAACCTTGGAAAGTATGATGATCCACCTGATTGG
  CAAGAGATTTTGACTTATTTCCGTGGATCTGAATTACAAAATTACTTTACCAAGATTCTCGAAGATGACC
  TAAAAGCCATTATCAAACCTCAATATGTAGACCAAATTCCCAAGGCTGCAAAGGGGACAGTGGGCTCTAT
  TCTGGACCGAAAAGATGAAACAAAGACACAGGCAATTGTATGTCAGCAGCTTGATTTAACTCACCTTAAA
  GAACGAAATGTCGAAGATCTTTCAGGAGGAGAGTTGCAGAGATTTGCTTGTGCTGTCGTTTGCATACAAA
  AGGCTGATATTTTTATGTTTGATGAACCTTCTAGTTACCTCGATGTCAAGCAACGTTTAAAGGCTGCCAT
  TACGATTCGATCTCTAATAAATCCAGATAGATATATCATTGTGGTGGAGCATGATCTAAGTGTATTAGAC
  TATCTCTCTGACTTCATCTGCTGTCTATATGGGGTACCGAGTGCTTATGGTGTTGTCACGATGCCTTTTA
  GTGTAAGAGAAGGCATAAATATATTTTTGGATGGCTATGTTCCAACAGAGAACCTGAGGTTCAGGGATGC
  GTCGCTTGTTTTTAAGGTAGCTGAGACAGCAAATGAAGAAGAAGTTAAAAAGATGTGCATGTATAAATAT
  CCCGGGATGAAGAAAAAGATGGGAGAGTTCGAGCTAGCAATTGTAGCTGGAGAGTTCACGGACTCTGAGA
  TCATGGTGATGCTGGGGGAGAATGGTACAGGTAAAACTACATTTATCAGAATGCTTGCTGGAAGGCTTAA
  ACCAGATGAAGGAGGAGAAGTGCCAGTTCTAAATGTCAGTTATAAGCCACAGAAAATCAGTCCCAAATCA
  ACAGGAAGTGTTCGCCAGTTACTGCATGAAAAGATCAGAGATGCTTACACGCATCCGCAGTTTGTGACTG
  ATGTAATGAAGCCCCTACAGATTGAAAACATCATTGACCAAGAGGTACAGACATTGTCTGGTGGTGAACT
  TCAGCGAGTAGCTTTAGCTCTTTGTTTGGGCAAACCTGCTGACGTCTATTTGATTGATGAACCTTCTGCA
  TATTTGGATTCTGAGCAAAGATTAATGGCAGCTCGGGTCGTCAAACGTTTCATTCTCCATGCAAAGAAGA
  CAGCTTTTGTTGTAGAACATGACTTCATCATGGCCACCTATCTAGCAGATCGCGTCATCGTTTTTGATGG
  TGTTCCATCAAAGAACACAGTTGCAAACAGTCCTCAGACTCTTTTGGCTGGCATGAACAAATTTTTGTCT
  CAGCTCGAAATTACATTCAGAAGAGACCCCAACAACTACAGGCCACGAATAAATAAGCTCAATTCAATCA
  AGGATGTAGAACAAAAGAAGAGTGGAAACTACTTTTTCTTGGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209296 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADKLTRIAIVNHDKCKPKKCRQECKKSCPVVRMGKLCIEVTPQSKIAWISETLCIGCGICIKKCPFGAL
  SIVNLPSNLEKETTHRYCANAFKLHRLPIPRPGEVLGLVGTNGIGKSTALKILAGKQKPNLGKYDDPPDW
  QEILTYFRGSELQNYFTKILEDDLKAIIKPQYVDQIPKAAKGTVGSILDRKDETKTQAIVCQQLDLTHLK
  ERNVEDLSGGELQRFACAVVCIQKADIFMFDEPSSYLDVKQRLKAAITIRSLINPDRYIIVVEHDLSVLD
  YLSDFICCLYGVPSAYGVVTMPFSVREGINIFLDGYVPTENLRFRDASLVFKVAETANEEEVKKMCMYKY
  PGMKKKMGEFELAIVAGEFTDSEIMVMLGENGTGKTTFIRMLAGRLKPDEGGEVPVLNVSYKPQKISPKS
  TGSVRQLLHEKIRDAYTHPQFVTDVMKPLQIENIIDQEVQTLSGGELQRVALALCLGKPADVYLIDEPSA
  YLDSEQRLMAARVVKRFILHAKKTAFVVEHDFIMATYLADRVIVFDGVPSKNTVANSPQTLLAGMNKFLS
  QLEITFRRDPNNYRPRINKLNSIKDVEQKKSGNYFFLDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015751
• ORF Size: 1800 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015751.2, NP_056566.2
• RefSeq Size: 3813 bp
• RefSeq ORF: 1800 bp
• Locus ID: 24015
• UniProt ID: P61222
• Cytogenetics: 8 C1
• MW: 67.3 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. [provided by RefSeq, Jul 2008]