Mmp14 (NM_008608) Mouse Tagged ORF Clone
CAT#: MR209110
- TrueORF®
Mmp14 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 14 (membrane-inserted) (Mmp14)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_008608" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Mmp14 |
Synonyms | AI325305; MMP-X1; MT-MMP-1; MT1-MM; MT1-MMP; sa; sabe |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209110 representing NM_008608
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCCGCCCCTCGACCCTCCCGCAGCCTCCTGCTCCCCCTGCTCACGCTTGGCACGGCGCTCGCCT CCCTCGGCTGGGCCCAAGGCAGCAACTTCAGCCCCGAAGCCTGGCTGCAGCAGTATGGCTACCTACCTCC AGGGGACCTGCGTACCCACACACAACGCTCACCCCAGTCACTCTCAGCTGCCATTGCCGCCATGCAAAAG TTCTATGGTTTACAAGTGACAGGCAAGGCTGATTTGGCAACCATGATGGCCATGAGGCGCCCTCGCTGTG GTGTTCCGGATAAGTTTGGGACTGAGATCAAGGCCAATGTTCGGAGGAAGCGCTATGCCATTCAGGGCCT CAAGTGGCAGCATAATGAGATCACTTTCTGCATTCAGAATTACACCCCTAAGGTGGGCGAGTATGCCACA TTCGAGGCCATTCGGAAGGCCTTCCGAGTATGGGAGAGTGCCACGCCACTGCGCTTCCGAGAAGTGCCCT ATGCCTACATCCGGGAGGGACATGAGAAGCAGGCTGACATCATGATCTTGTTTGCTGAGGGTTTCCACGG CGACAGTACACCCTTTGATGGTGAAGGAGGGTTCCTGGCTCATGCCTACTTCCCAGGCCCCAATATTGGA GGGGATACCCACTTTGATTCTGTCGAGCCCTGGACTGTCCAAAATGAGGATCTAAATGGGAATGACATCT TCTTGGTGGCTGTGCATGAGTTGGGGCATGCCCTAGGCCTGGAACATTCCAATGATCCCTCCGCCATCAT GGCCCCCTTTTACCAGTGGATGGACACAGAGAACTTCGTGTTGCCTGATGACGATCGCCGTGGCATCCAG CAACTTTATGGAAGCAAGTCAGGGTCACCCACAAAGATGCCCCCTCAACCCAGAACTACCTCTCGGCCCT CTGTCCCAGATAAGCCCAAAAACCCCGCCTATGGGCCCAACATCTGTGACGGGAACTTTGACACCGTGGC CATGCTCCGAGGAGAGATGTTTGTCTTCAAGGAGCGATGGTTCTGGCGGGTGAGGAATAACCAAGTGATG GATGGATTCCCAATGCCCATTGGCCAATTCTGGAGGGGCCTGCCTGCATCCATCAATACTGCCTACGAGA GGAAGGATGGCAAATTTGTCTTCTTCAAAGGAGATAAGCACTGGGTGTTTGACGAAGCCTCCCTGGAACC CGGGTACCCCAAGCACATTAAGGAGCTGGGCCGAGGGCTGCCCACGGACAAGATCGATGCAGCTCTCTTC TGGATGCCCAATGGGAAGACCTACTTCTTCCGGGGCAATAAGTACTACCGGTTCAATGAAGAATTCAGGG CAGTGGACAGCGAGTACCCTAAAAACATCAAAGTCTGGGAAGGAATCCCTGAATCTCCCAGGGGGTCATT CATGGGCAGTGATGAAGTCTTCACATACTTCTACAAGGGAAACAAATACTGGAAGTTCAACAACCAGAAG CTGAAGGTAGAGCCAGGGTATCCCAAGTCAGCTCTGCGGGACTGGATGGGCTGCCCTTCGGGGGGCCGGC CCGATGAGGGGACTGAGGAGGAGACGGAGGTGATCATCATTGAGGTGGATGAGGAGGGCAGTGGAGCTGT GAGTGCGGCCGCCGTGGTCCTGCCGGTACTACTGCTGCTCCTGGTACTGGCAGTGGGCCTCGCTGTCTTC TTCTTCAGACGCCATGGGACGCCCAAGCGACTGCTTTACTGCCAGCGTTCGCTGCTGGACAAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209110 representing NM_008608
Red=Cloning site Green=Tags(s) MSPAPRPSRSLLLPLLTLGTALASLGWAQGSNFSPEAWLQQYGYLPPGDLRTHTQRSPQSLSAAIAAMQK FYGLQVTGKADLATMMAMRRPRCGVPDKFGTEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYAT FEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMILFAEGFHGDSTPFDGEGGFLAHAYFPGPNIG GDTHFDSVEPWTVQNEDLNGNDIFLVAVHELGHALGLEHSNDPSAIMAPFYQWMDTENFVLPDDDRRGIQ QLYGSKSGSPTKMPPQPRTTSRPSVPDKPKNPAYGPNICDGNFDTVAMLRGEMFVFKERWFWRVRNNQVM DGFPMPIGQFWRGLPASINTAYERKDGKFVFFKGDKHWVFDEASLEPGYPKHIKELGRGLPTDKIDAALF WMPNGKTYFFRGNKYYRFNEEFRAVDSEYPKNIKVWEGIPESPRGSFMGSDEVFTYFYKGNKYWKFNNQK LKVEPGYPKSALRDWMGCPSGGRPDEGTEEETEVIIIEVDEEGSGAVSAAAVVLPVLLLLLVLAVGLAVF FFRRHGTPKRLLYCQRSLLDKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008608 |
ORF Size | 1746 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008608.2, NM_008608.3, NM_008608.4, NP_032634.2, NP_032634.3 |
RefSeq Size | 2597 bp |
RefSeq ORF | 1749 bp |
Locus ID | 17387 |
UniProt ID | P53690 |
Cytogenetics | 14 27.79 cM |
MW | 66.4 kDa |
Gene Summary | This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein exhibit craniofacial dysmorphism, arthritis, osteopenia, dwarfism, and fibrosis of soft tissues. [provided by RefSeq, Feb 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219253 | Mmp14 (untagged) - Mouse matrix metallopeptidase 14 (membrane-inserted) (Mmp14), (10ug) |
USD 543.00 |
|
MG209110 | Mmp14 (tGFP-tagged) - Mouse matrix metallopeptidase 14 (membrane-inserted) (Mmp14) |
USD 742.00 |
|
MR209110L3 | Lenti ORF clone of Mmp14 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 14 (membrane-inserted) (Mmp14) |
USD 842.00 |
|
MR209110L4 | Lenti ORF clone of Mmp14 (mGFP-tagged) - Mouse matrix metallopeptidase 14 (membrane-inserted) (Mmp14) |
USD 842.00 |
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