Agfg1 (NM_010472) Mouse Tagged ORF Clone
CAT#: MR208867
- TrueORF®
Agfg1 (Myc-DDK-tagged) - Mouse ArfGAP with FG repeats 1 (Agfg1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_010472" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Agfg1 |
Synonyms | AU045498; C85612; C130049H11Rik; D730048C23Rik; Hrb; RAB; Rip |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208867 representing NM_010472
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCAGCGCGAAGCGGAAGCAGGAGGAGAAGCACCTGAAGATGCTGCGGGACATGACGGGGCTGC CGCACAACCGCAAGTGTTTCGACTGCGACCAGCGCGGCCCCACCTACGTGAACATGACGGTCGGCTCGTT CGTGTGCACCTCCTGCTCCGGCAGCCTGAGAGGGTTAAATCCACCTCACAGGGTGAAATCTATCTCCATG ACAACATTTACACAACAGGAAATTGAATTCCTACAAAAACATGGAAATGAAGTCTGCAAACAGATATGGC TAGGATTATTTGATGACAGATCTTCAGCAATTCCAGACTTCAGGGATCCACAAAAAGTGAAAGAGTTTCT ACAAGAGAAATATGAAAAGAAAAGATGGTATGTTCCGCCAGAGCAAGCCAAAGTGGTGGCCTCGGTTCAT GCGTCTATTTCAGGGTCTTCTGCCAGCAGCACAAGCAGCACCCCGGAGGTCAAGCCCCTGAAATCCCTGT TGGGAGAGTCTGCACCCGCACTGCACCTAAATAAGGGCACACCTAGTCAGTCCCCAGTTGTAGGTCGCTC TCAAGGGCAGCAGCAGGAGAAGAAGCAGTTTGACCTTTTGAGTGATCTTGGCTCAGACATCTTTGCTGCT CCAGCTCCTCAGTCAACAGCTACAGCCAATTTTGCTAACTTTGCACACTTTAACAGTCATGCAGCTCAGA ATTCTGCAAATGCAGATTTTGCAAACTTTGATGCATTTGGACAGTCTAGTGGTTCGAGTAATTTTGGAGG TTTCCCCACAGCAAGTCACTCTTCTTTTCAGCCCCAGACTACAGGTGGAAGTGCTGGATCAGTAAATGCT AATTTTGCTCATTTTGATAACTTCCCTAAATCCTCCAGTGCTGATTTTGGAACCTTCAGTACATCCCAGA GTCATCAGACAGCATCAACTGTTAGTAAAGTTTCAACAAACAAAGCTGGTTTACAGACAGCAGACAAATA TGCGGCACTTGCTAATTTAGACAATATCTTCAGTGCTGGGCAAGGAGGTGATCAAGGGAGTGGTTTTGGG ACCACGGGTAAAGCTCCTGTTGGTTCTGTGGTTTCAGTTCCCAGTCATTCAAGTGCATCTTCAGACAAGT ATGCAGCCCTGGCAGAGTTAGACAGCGTGTTCAGCTCTGCAGCCACCTCCAGTAATGCGTACACGCCCAC CAGTAATGCTAGCAGCAGTGTCTTTGGAACAGTGCCTGTGGGTGCCTCTGCTCAGACACAACCTGCTTCA AGTGGTCCTGCTCCGTTTGGGGCTACGCCTTCTACGAATCCATTTGTTGCTGCTACTGGTCCTTCTGCGG CATCGTCTACAAACCCATTTCAGACCAATGCCAGAGGAGCAACAGCGGCAACCTTTGGCACCGCATCTAT GAGTATGCCAGCAGGATTTGGCACTCCTGCCCAGTATAGTCTCCCTACCAGCTTTAGTGGCAGTTTCCAG CAGCCTGCCTTCCCAGCCCAAGCAGCTTTCCCTCAGCAGACAGCCTTTTCTCAACAGCCCAATGGTTTCG CCACGTTTGGACAAACAAAACCAGTGGTAACCCCTTTCGGTCAAGTAGCAGCTGCTGGAGTGTCTAGTAA TCCTTTTATGACTGGTGCGCCGACAGGACAACTTCCAACAGGAAGCTCATCCACCAACCCTTTCTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208867 representing NM_010472
Red=Cloning site Green=Tags(s) MAASAKRKQEEKHLKMLRDMTGLPHNRKCFDCDQRGPTYVNMTVGSFVCTSCSGSLRGLNPPHRVKSISM TTFTQQEIEFLQKHGNEVCKQIWLGLFDDRSSAIPDFRDPQKVKEFLQEKYEKKRWYVPPEQAKVVASVH ASISGSSASSTSSTPEVKPLKSLLGESAPALHLNKGTPSQSPVVGRSQGQQQEKKQFDLLSDLGSDIFAA PAPQSTATANFANFAHFNSHAAQNSANADFANFDAFGQSSGSSNFGGFPTASHSSFQPQTTGGSAGSVNA NFAHFDNFPKSSSADFGTFSTSQSHQTASTVSKVSTNKAGLQTADKYAALANLDNIFSAGQGGDQGSGFG TTGKAPVGSVVSVPSHSSASSDKYAALAELDSVFSSAATSSNAYTPTSNASSSVFGTVPVGASAQTQPAS SGPAPFGATPSTNPFVAATGPSAASSTNPFQTNARGATAATFGTASMSMPAGFGTPAQYSLPTSFSGSFQ QPAFPAQAAFPQQTAFSQQPNGFATFGQTKPVVTPFGQVAAAGVSSNPFMTGAPTGQLPTGSSSTNPFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010472 |
ORF Size | 1677 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010472.3 |
RefSeq Size | 3137 bp |
RefSeq ORF | 1680 bp |
Locus ID | 15463 |
UniProt ID | Q8K2K6 |
Cytogenetics | 1 C5 |
MW | 57.9 kDa |
Gene Summary | Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204232 | Agfg1 (untagged) - Mouse ArfGAP with FG repeats 1 (Agfg1), (10ug) |
USD 521.00 |
|
MG208867 | Agfg1 (tGFP-tagged) - Mouse HIV-1 Rev binding protein (Hrb) |
USD 720.00 |
|
MR208867L3 | Lenti ORF clone of Agfg1 (Myc-DDK-tagged) - Mouse ArfGAP with FG repeats 1 (Agfg1) |
USD 820.00 |
|
MR208867L4 | Lenti ORF clone of Agfg1 (mGFP-tagged) - Mouse ArfGAP with FG repeats 1 (Agfg1) |
USD 820.00 |
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