Ptpn5 (NM_001163565) Mouse Tagged ORF Clone

CAT#: MR208630

Ptpn5 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 5 (Ptpn5), transcript variant 2

Product Images

Circular map for MR208630

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptpn5
• Synonyms: Step
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208630 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTGTAGTGAGAGGCTGTTGGGTCTCCCCCAGCCGGTAGAGATGGAAGCACCGGACGAGGCCGAAG
  GACTCCCCAGCAAGCAGAAAGAGATGCCACCACCCCCGCCACCCTCACCGCCCTCTGAGCCAGCTCAGAA
  GCTGCCACCTCAAGGCGCTGGGAGCCACTCCCTCACCGTCAGAAGCAGCCTGTGCCTGTTTGCTGCCTCT
  CAGTTCCTGCTTGCCTGTGGGGTGCTCTGGCTCAGTGGCCATGGCCACTCCTGGCTGCAGAACACCACAG
  ACCTCATCTCCTCCTCGCTCACAGTGTTGAACCATCTGGGACCTGTGGCCTGGCTGGGTTCTGGGACCTG
  GGGGATACCAAGTCTGCTGCTAGTCTCTCTGACTGTGAGCCTGGTCATCGTCACCACCCTGGTGTGGCAC
  CTCCTCAAGGCACCCCCAGAGCCACCTGCCCCACTGCCCCCAGAGGACAGGCGTCAATCAGTGAGCCGGC
  AGCCTTCCTTCACCTACTCAGAGTGGATGGAGGAGAAGGTAGAGGATGACTTCCTGGACCTGGACGCGGT
  GCCCGAGACACCTGTGTTTGACTGTGTGATGGACATCAAGCCTGAGACTGATCCTGCCTCATTGACTGTC
  AAGTCCATGGGTCTACAGGAGAGGAGAGGATCCAATGTCTCCTTGACCCTGGACATGTGTACTCCTGGCT
  GCAATGAGGAGGGCTTCGGCTACCTGGTGTCTCCACGAGAAGAGTCAGCCCATGAGTATCTGCTCAGCGC
  CTCCCGTGTCCTCCGGGCAGAAGAGCTACATGAAAAGGCTCTGGACCCTTTCTTGCTGCAGGCGGAATTC
  TTTGAAATCCCCATGAACTTTGTGGATCCAAAAGAGTATGACATCCCAGGGCTGGTGCGGAAGAATCGGT
  ACAAAACCATCCTTCCCAATCCTCACAGCAGGGTACGTCTGACGTCACCAGACCCTGAAGATCCTCTGAG
  TTCCTACATCAATGCCAACTACATCCGGGGCTACAGTGGGGAGGAGAAGGTGTACATCGCCACGCAGGGA
  CCCATCGTCAGCACTGTGGCCGACTTTTGGCGCATGGTGTGGCAGGAGCGCACACCCATCATCGTCATGA
  TCACCAACATCGAGGAGATGAACGAGAAGTGCACAGAATACTGGCCAGAAGAGCAGGTGGTCCATGACGG
  CGTGGAGATCACCGTGCAGAAAGTCATCCACACGGAGGACTACCGGCTTCGACTCATCTCCCTCAGGAGA
  GGGACTGAAGAGCGAAGCTTGAAGCATTACTGGTTCACATCCTGGCCTGACCAGAAGACCCCCGACCGGG
  CTCCCCCACTCCTGCACCTGGTACGGGAAGTGGAGGAGGCAGCCCAACAAGAGGGACCCCATTGTTCCCC
  AATCATCGTTCACTGCAGTGCAGGGATTGGGAGAACTGGCTGCTTCATTGCCACCAGCATCTGCTGCCAG
  CAGCTGCGACGTGAGGGCGTGGTGGACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGTGGCA
  TGATCCAGACATGCGAACAGTACCAGTTTGTGCACCACGCCATGAGCCTCTATGAGAAGCAGCTGTCCCT
  CCAGTCCTCAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208630 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCCSERLLGLPQPVEMEAPDEAEGLPSKQKEMPPPPPPSPPSEPAQKLPPQGAGSHSLTVRSSLCLFAAS
  QFLLACGVLWLSGHGHSWLQNTTDLISSSLTVLNHLGPVAWLGSGTWGIPSLLLVSLTVSLVIVTTLVWH
  LLKAPPEPPAPLPPEDRRQSVSRQPSFTYSEWMEEKVEDDFLDLDAVPETPVFDCVMDIKPETDPASLTV
  KSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLVSPREESAHEYLLSASRVLRAEELHEKALDPFLLQAEF
  FEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVRLTSPDPEDPLSSYINANYIRGYSGEEKVYIATQG
  PIVSTVADFWRMVWQERTPIIVMITNIEEMNEKCTEYWPEEQVVHDGVEITVQKVIHTEDYRLRLISLRR
  GTEERSLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCSPIIVHCSAGIGRTGCFIATSICCQ
  QLRREGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHAMSLYEKQLSLQSSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163565
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163565.1, NP_001157037.1
• RefSeq Size: 3049 bp
• RefSeq ORF: 1626 bp
• Locus ID: 19259
• UniProt ID: P54830
• Cytogenetics: 7 30.7 cM
• MW: 60.8 kDa
• Gene Summary: May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.[UniProtKB/Swiss-Prot Function]