Gpatch2 (NM_026367) Mouse Tagged ORF Clone

CAT#: MR208457

Gpatch2 (Myc-DDK-tagged) - Mouse G patch domain containing 2 (Gpatch2)

Product Images

Circular map for MR208457

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpatch2
• Synonyms: 5830433G22Rik; 5830436K05Rik; AI427714; AI447508; AW107440; AW491060; Gpatc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208457 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGGCCGACGGGCGCCCAGCGATCGGAACCGCCGCCGGGAAGAGCTGGCATTTCAGTCGAACAA
  TGGAAGAGCTTGTTCACGATCTTGTCTCTGCACTGGAAGAGAGCTCTGAGCAAGCCCGAGGTGGATTTGC
  TGAAACTGGAGAACATTCTCGAAATCTGTCTTGCCCTCTGAAACGCCAGGCCCGGAAAAGGAGAGGGAGG
  AAGCGGAGATCCTACAATGTTCACCACCCGTGGGAGACAGGCCACTGCTTAAGTGAAGGCTCTGATTCTA
  GTTTAGAAGAACCAAGTAAGGACTATAGAGAGAAGCACAGCAATAATAAAAAGGACCGCAGTGACTCTGA
  TGACCAGATGTTAGTGGCGAAGCGGAGGCCATCTTCAAACCTAAGCAGCAGCGTTCGAGGTAAGCGGCTT
  CTGTGGCACGAGTCTGACTTTGCCGTGGACAGCCTTGGGAACAGAACGCTGCGCCGGAGGAGGAAGGTGA
  AGCGCATGGCCGTGGATCTCCCGCAGGACGTCTCCAGCAAAAGGACAATGACCCAGCTGCCAGAAGGCTG
  CAGAGATCAGGACATGGACAATGATAGAGCCAGCCAGTATCCAGAGTTTACCCGGAAGAAAGTTAAGAAA
  AGGAAGTTGAAAGGGATTAGGCCAGGACCGAAAACCCAGGAGGAAGGAGGAGTTTTGGAGAGTGAAGAAA
  GAAGCCAGCCCAACAAGGACAGGATGGAGTACGAGGAACAGAAAGCCTCGGATGAGCTCAGGAGCGAAAG
  TGACACCAGCAGTCTCAGCAGCACTGACGCGGGCTTGTTCACCAACGATGAGGGAAGACAAGGTGATGAT
  GAGCAGAGTGACTGGTTCTATGAGAAGGAGTCAGGCGGAGCGTGCGGGATTGCTGGAGTCGTGCCCTGGT
  GGGAAAAGGATGAGCCAGCAGAGCTGGACACCAACCTGCCCGACCCTGTGTTTGAGAGCATCTTAAGTGG
  CTCCTTCCCTCTCATGTCCCATCCTGGCAGAGGAGGTTTCCAAGCTAGACTCAGTCGCCTTCATGGAACG
  CCTTCAAAGAATATTAAAAAGTCTTCAGGGGCTCCACCTTCAATGCTGCCTGCTCCTGGCCCTGGCAGTA
  ACAAGAGGATGGTTCACTTCTCCCCAGACGCCCATCGCCATGACCACTGGTTTAGCCCTGGGGCTAGGAC
  AGAGCACGGCCAGCATCAGCTTCTGAGAGATAACCGAGCGGAAAGAGGACACAAGAAAAGCTGCTCCCTG
  AAAACAGCCAGCAGGCAGACAAGCATGCATTTAGGATCCTTGTGCACAGGAGACATCAAAAGGAGAAGGA
  AAGCTGCCCCGTTGCCTGGACCCACTGCAGCAGGAATTGTGGGTGAGAACGCCCAGCCGATCCTAGAGAG
  CAACATCGGGAACCGCATGCTTCAGAGTATGGGATGGACACCCGGGTCAGGCCTCGGGCGAGATGGCAGA
  GGGATCGCGGAGCCAGTTCAAGCCGTTCAGAGGCCGAAAGGGTTAGGACTTGGATTTCCTCTACCAAAAA
  GCTCCCCCACCAGCCCTGCCCCCACATCAGGAAACCCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208457 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFGADGRPAIGTAAGKSWHFSRTMEELVHDLVSALEESSEQARGGFAETGEHSRNLSCPLKRQARKRRGR
  KRRSYNVHHPWETGHCLSEGSDSSLEEPSKDYREKHSNNKKDRSDSDDQMLVAKRRPSSNLSSSVRGKRL
  LWHESDFAVDSLGNRTLRRRRKVKRMAVDLPQDVSSKRTMTQLPEGCRDQDMDNDRASQYPEFTRKKVKK
  RKLKGIRPGPKTQEEGGVLESEERSQPNKDRMEYEEQKASDELRSESDTSSLSSTDAGLFTNDEGRQGDD
  EQSDWFYEKESGGACGIAGVVPWWEKDEPAELDTNLPDPVFESILSGSFPLMSHPGRGGFQARLSRLHGT
  PSKNIKKSSGAPPSMLPAPGPGSNKRMVHFSPDAHRHDHWFSPGARTEHGQHQLLRDNRAERGHKKSCSL
  KTASRQTSMHLGSLCTGDIKRRRKAAPLPGPTAAGIVGENAQPILESNIGNRMLQSMGWTPGSGLGRDGR
  GIAEPVQAVQRPKGLGLGFPLPKSSPTSPAPTSGNPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026367
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026367.3, NP_080643.3
• RefSeq Size: 4565 bp
• RefSeq ORF: 1584 bp
• Locus ID: 67769
• UniProt ID: Q7TQC7
• Cytogenetics: 1 H5
• MW: 58.2 kDa
• Gene Summary: Enhances the ATPase activity of DHX15 in vitro.[UniProtKB/Swiss-Prot Function]