Athl1 (BC023151) Mouse Tagged ORF Clone

CAT#: MR208359

Athl1 (Myc-DDK-tagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (cDNA clone MGC:28193 IMAGE:3988807)

Product Images

Circular map for MR208359

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Athl1
• Synonyms: MGC28193
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208359 representing BC023151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTATGGATGCCTGTCCCCCCAGCCTTGACCCTTGGGGAAGAGGAGAAGGACAGGACCTGGGAGTTTC
  TGACTGTTGTGGGTAGCAGCCAGGCTGAAGCCCAGGACTGCTTTGCTGAGGCCCTACAGCTGCAGACCAG
  GGGTGTTCTGTATACAATCCATGCAGACTCCTGGGGCCGGCTATGGGCAGGCTGTGGCCTGGATGTGGCT
  GGGCCCCTGGCCCTGCGCCAGGCCCTTCGTGGCTCTCTCTACTACCTGTTCAGTGAGCTTCCCCAGCCTG
  GCACTCAAGGATTCATCAGCCATGGGCTTAGCCCCGGAGGCCTCTCCAATGGGAGTAAGGAGGAATGCTA
  CTGGGGCCATATCTTCTGGGATCAGGACATCTGGATGTTCCCAAATATTTTGATGTTCCACCCAGAAGCC
  GCCAGGGCCATCCTAGAATACCGAGTCCGTACGCTGGGAGGAGCCCTGAAGAATGGCCAGAACCTGGGCT
  ACCAGGGAGCCAAGTTTGCTTGGGAGAGTGCAAGCACTGGCCTGGAGGTCTGCCCTGAGGACATTTATGG
  GACCCAGGAGATCCATATCAATGGGGCTGTGGCCTTGGCCTTTCAGCTGTATTACTATTACACTCAGGAC
  TCGAAGCTCTTCCAAGAGGATGGTGGCTGGGATGTGGTCAGTTCTGTGGCTGAGTTTTGGTGTAGCCGTG
  TAGAATGGAGCTCCCAGGACAAGATGTACCACCTGAAAGGAGTCATGCCCCCCGATGAGTACCATTCAGG
  AGTCAACAACTCTGTCTACACCAATGTCCTGGTCCAGAACAGCCTGCACTTCGCTGCTGCTTTGGCCAAA
  GACTTGGGTTTGCCTATCCGCAAACAGTGGCTGGAGGTGGCTGATAGGATCAAGATACCATTTGACTCTG
  AGCAGAACTTCCACCCTGAGTTTGATGGCTATGAACGTGGAGAAGAGGTGAAGCAGGCAGATGTTGTGCT
  CCTGGGGTACCCAGTCCCCTTTCCCCTGACACCTGACATCCGAAGGAAAAACTTGGAGACTTATGAGGCC
  GTGACACCCCCCCAGGGCCCTGCTATGACCTGGAGCATGTTTGCTGTGGGCTGGATGGAACTGAGGGACC
  CCTCAAGGGCTCAGGTCCACCTGAGCAGGAGTTTCGCCAATGTCACTGAGCCCTTCAAGGTGTGGACGGA
  GAATGCAGACGGGTCTGGTGCAGTGAACTTCTTGACTGGCATGGGGGGCTTCCTGCAGGCAGCACTCTTC
  GGGTGCACAGGATTCAGGATCACTGAGGCAGGTGTGACCTTTGACCCCCTGTGTCCAGACCTGGTCTCCA
  GAGTATCTGTCTCTGGTATCTCCTACCTGGGGAACAAGATCAACTTCGCCTTTTCCAAGGACTCGGTGAC
  ACTTGAGGTCACAGCCCGTGCAGAGCCCTGGGCACCCCTACTAGAAGCTGAGCTGTGGCCATCGCTGGCT
  CACCTTCCCCTGACACCAGAAGGTCTCCTTTCCCCACTCAGCTGGCCGGATACAAAGGTCATCCCCATAG
  CTGCCCAGAAATTCTTCCGTGGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208359 representing BC023151
  Red=Cloning site Green=Tags(s)
  
  MLWMPVPPALTLGEEEKDRTWEFLTVVGSSQAEAQDCFAEALQLQTRGVLYTIHADSWGRLWAGCGLDVA
  GPLALRQALRGSLYYLFSELPQPGTQGFISHGLSPGGLSNGSKEECYWGHIFWDQDIWMFPNILMFHPEA
  ARAILEYRVRTLGGALKNGQNLGYQGAKFAWESASTGLEVCPEDIYGTQEIHINGAVALAFQLYYYYTQD
  SKLFQEDGGWDVVSSVAEFWCSRVEWSSQDKMYHLKGVMPPDEYHSGVNNSVYTNVLVQNSLHFAAALAK
  DLGLPIRKQWLEVADRIKIPFDSEQNFHPEFDGYERGEEVKQADVVLLGYPVPFPLTPDIRRKNLETYEA
  VTPPQGPAMTWSMFAVGWMELRDPSRAQVHLSRSFANVTEPFKVWTENADGSGAVNFLTGMGGFLQAALF
  GCTGFRITEAGVTFDPLCPDLVSRVSVSGISYLGNKINFAFSKDSVTLEVTARAEPWAPLLEAELWPSLA
  HLPLTPEGLLSPLSWPDTKVIPIAAQKFFRGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023151
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023151
• RefSeq Size: 2723 bp
• RefSeq ORF: 1568 bp
• Locus ID: 212974
• Cytogenetics: 7 F5
• MW: 99.8 kDa
• Gene Summary: Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.[UniProtKB/Swiss-Prot Function]