Clca1 (BC010260) Mouse Tagged ORF Clone
CAT#: MR208248
- TrueORF®
Clca1 (Myc-DDK-tagged) - Mouse chloride channel calcium activated 1 (cDNA clone MGC:11678 IMAGE:3710379)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"BC010260" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Clca1 |
Synonyms | Cacc; Clca1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208248 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCCAGGGCTGCAGGTCCTTCTGTTCCTCACCCTGCATCTCCTGCAGAACACAGAGAGCTCCATGG TGCATCTCAACAGCAATGGATACGAGGGTGTGGTCATTGCCATTAACCCCAGTGTGCCGGAGGACGAAAG GCTCATCCCAAGCATAAAGGAAATGGTAACTCAAGCCTCTACCTACCTGTTTGAAGCCAGCCAAGGAAGA GTTTATTTCAGAAACATAAGCATATTAGTCCCGATGACCTGGAAGTCAAAACCTGAGTACTTAATGCCAA AACGAGAATCGTATGACAAAGCAGACGTCATAGTTGCGGATCCTCACCTGCAACATGGAGACGACCCCTA CACCCTTCAGTATGGACAGTGTGGGGACAGAGGACAGTACATACACTTCACTCCAAACTTCCTACTCACT GATAACTTGCGTATCTACGGACCCCGAGGCAGAGTCTTTGTCCATGAGTGGGCCCATCTCCGGTGGGGAG TATTTGATGAGTATAACGTGGACCAGCCTTTCTACATGTCTAGAAAGAACACTATAGAAGCAACAAGGTG TTCCACCAGGATCACTGGCACCAATGTGGTTCACAACTGTGAGAGAGGCAACTGTGTCACAAGGGCGTGC CGGCGTGACTCGAAGACACGGCTGTATGAACCCAAATGTACATTTATCCCAGACAAAATACAGACGGCTG GGGCCTCCATAATGTTCATGCAAAACCTTAATTCTGTGGTTGAATTTTGCACAGAAAAAAACCACAATGC AGAAGCCCCAAACCTACAAAACAAAATGTGCAATCGCAGAAGCACGTGGGATGTAATCAAGACGTCTGCT GACTTTCAGAATGCCCCTCCCATGAGAGGAACAGAAGCCCCTCCTCCACCTACATTTTCACTGCTCAAGT CCAGAAGGCGAGTGGTGTGCTTGGTGCTGGATAAATCTGGAAGCATGGACAAAGAAGACCGTCTTATTCG AATGAATCAAGCAGCAGAACTGTACTTAACTCAAATTGTGGAAAAGGAGTCTATGGTTGGATTAGTCACA TTTGACAGCGCTGCCCACATCCAAAATTATCTAATAAAAATAACGAGTAGTAGTGACTACCAAAAGATCA CCGCAAACCTCCCCCAACAGGCTTCTGGTGGAACTTCAATTTGCCATGGACTCCAGGCAGGATTTCAGGC AATTACCTCCAGTGACCAGAGCACTTCCGGTTCTGAGATCGTATTGCTGACAGATGGGGAAGATAATGGA ATACGTTCCTGCTTTGAGGCCGTCTCTCGCAGCGGTGCCATCATCCACACCATCGCTCTGGGGCCTTCGG CTGCCCGAGAACTGGAGACTCTGTCGGACATGACAGTTGGAGAGCAAAGCCTTCGATGTCAGAGCAGGGG CATGGATAAACGGTACAGTACCTCTGGACAGTACCGTCGGCAACGACACGTTCTTTGTTATCACCTGGAT GGTAAAAAAGCCAGAAATCATTCTTCAAGATCCAAAAGGAAAAAAATATACAACCTCAGATTTCCAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208248 protein sequence
Red=Cloning site Green=Tags(s) MVPGLQVLLFLTLHLLQNTESSMVHLNSNGYEGVVIAINPSVPEDERLIPSIKEMVTQASTYLFEASQGR VYFRNISILVPMTWKSKPEYLMPKRESYDKADVIVADPHLQHGDDPYTLQYGQCGDRGQYIHFTPNFLLT DNLRIYGPRGRVFVHEWAHLRWGVFDEYNVDQPFYMSRKNTIEATRCSTRITGTNVVHNCERGNCVTRAC RRDSKTRLYEPKCTFIPDKIQTAGASIMFMQNLNSVVEFCTEKNHNAEAPNLQNKMCNRRSTWDVIKTSA DFQNAPPMRGTEAPPPPTFSLLKSRRRVVCLVLDKSGSMDKEDRLIRMNQAAELYLTQIVEKESMVGLVT FDSAAHIQNYLIKITSSSDYQKITANLPQQASGGTSICHGLQAGFQAITSSDQSTSGSEIVLLTDGEDNG IRSCFEAVSRSGAIIHTIALGPSAARELETLSDMTVGEQSLRCQSRGMDKRYSTSGQYRRQRHVLCYHLD GKKARNHSSRSKRKKIYNLRFPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC010260 |
ORF Size | 1539 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC010260, AAH10260 |
RefSeq Size | 3201 bp |
RefSeq ORF | 1541 bp |
Locus ID | 12722 |
Cytogenetics | 3 69.06 cM |
MW | 58 kDa |
Gene Summary | Plays a role in modulating chloride current across the plasma membrane in a calcium-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200738 | Clca1 (untagged) - Mouse chloride channel calcium activated 1 (cDNA clone MGC:11678 IMAGE:3710379), (10ug) |
USD 479.00 |
|
MG208248 | Clca1 (tGFP-tagged) - Mouse chloride channel calcium activated 1 (cDNA clone MGC:11678 IMAGE:3710379) |
USD 678.00 |
|
MR208248L3 | Lenti ORF clone of Clca1 (Myc-DDK-tagged) - Mouse chloride channel calcium activated 1 (cDNA clone MGC:11678 IMAGE:3710379) |
USD 778.00 |
|
MR208248L4 | Lenti ORF clone of Clca1 (mGFP-tagged) - Mouse chloride channel calcium activated 1 (cDNA clone MGC:11678 IMAGE:3710379) |
USD 778.00 |
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