Ina (NM_146100) Mouse Tagged ORF Clone

CAT#: MR208054

Ina (Myc-DDK-tagged) - Mouse internexin neuronal intermediate filament protein, alpha (Ina)

Product Images

Circular map for MR208054

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ina
• Synonyms: AV028420; NF-66; NF66
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208054 representing NM_146100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCGGATCGGAGCACTATTTGTGCTCCGCCTCCTCCTACCGCAAGGTGTTCGGGGACAGCTCGC
  GCCTCTCCGCGCGCCTGTCCGGGCCGGGAGGCTCGGGCAGCTTCCGCTCGCAGTCGCTGTCCCGCAGCAA
  TGTGGCCTCCACGGCCGCCTGCTCCTCGGCCTCGTCCCTCGGCCTGGGCCTGGCCTACCGCCGCCTGCCG
  GCCTCCGACGGGCTGGACCTGAGCCAGGCGGCGGCGCGCACCAACGAGTACAAGATCATCCGCACTAACG
  AGAAGGAGCAGCTGCAGGGCCTCAACGACCGCTTCGCCGTGTTCATCGAGAAGGTGCACCAGCTGGAGAC
  GCAGAACCGTGCGCTCGAGGCCGAGCTGGCCGCGCTGCGCCAGCGCCACGCCGAGCCGTCGCGCGTCGGC
  GAGCTCTTCCAGCGCGAGCTGCGCGAGCTGCGCGCGCAGCTGGAGGAGGCGAGCTCGGCGCGCGCGCAGG
  CCCTGCTGGAGCGCGATGGGCTGGCCGAGGAGGTGCAGCGACTGCGGGCGCGCTGCGAGGAGGAGAGCCG
  CGGGCGCGAAGGCGCCGAGCGCGCCCTGAAGGCGCAGCAGCGCGACGTGGACGGCGCCACCCTGGCCCGC
  CTGGATCTGGAGAAGAAGGTGGAGTCGCTGCTGGACGAGCTGGCTTTCGTGCGCCAGGTCCACGACGAGG
  AGGTGGCCGAGCTCCTGGCCACGTTGCAGGCGTCTTCGCAAGCCGCCGCCGAGGTGGACGTGGCCGTGGC
  TAAACCAGACCTGACTTCGGCGCTGAGGGAGATCCGCGCGCAGTATGAGTCCCTGGCCGCTAAGAACCTG
  CAGTCCGCCGAGGAGTGGTACAAGTCCAAGTTCGCCAACCTAAACGAGCAGGCTGCGCGCAGCACCGAAG
  CCATCCGAGCCAGCCGAGAGGAGATCCACGAGTACCGGCGCCAGCTCCAGGCACGTACCATTGAGATAGA
  GGGTCTGCGCGGAGCCAATGAGTCCCTGGAGAGGCAGATCTTGGAACTGGAGGAGCGGCACAGCGCTGAG
  GTGGCCGGCTACCAGGACAGCATTGGGCAGCTGGAAAGTGACCTGAGGAACACAAAAAGCGAGATGGCAC
  GCCACCTTCGGGAATACCAGGACTTGCTCAATGTCAAAATGGCCCTTGACATTGAGATCGCAGCTTACAG
  GAAGCTCCTGGAAGGTGAAGAGACGCGGTTTAGCACCGGCGGGTTAAGCATCTCGGGGCTGAATCCACTG
  CCCAATCCCAGTTATTTGCTCCCTCCCAGAATCCTTAGCTCTACAGCCTCCAAAGTCTCCTCTGCCGGGC
  TGTCCCTGAAAAAGGAGGAAGAAGAGGAGGAGGAAGAGGCCTCTAAGGAAGTCAGTAAGAAAACATCCAA
  GGTAGGGGAAGGTTTCGAAGAGACACTGGGGGAAGCGGTAATATCTACGAAGAAAACTGGAAAGTCGGCT
  ACAGAAGAAAGTACCAGTTCAAGCCAAAAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208054 representing NM_146100
  Red=Cloning site Green=Tags(s)
  
  MSFGSEHYLCSASSYRKVFGDSSRLSARLSGPGGSGSFRSQSLSRSNVASTAACSSASSLGLGLAYRRLP
  ASDGLDLSQAAARTNEYKIIRTNEKEQLQGLNDRFAVFIEKVHQLETQNRALEAELAALRQRHAEPSRVG
  ELFQRELRELRAQLEEASSARAQALLERDGLAEEVQRLRARCEEESRGREGAERALKAQQRDVDGATLAR
  LDLEKKVESLLDELAFVRQVHDEEVAELLATLQASSQAAAEVDVAVAKPDLTSALREIRAQYESLAAKNL
  QSAEEWYKSKFANLNEQAARSTEAIRASREEIHEYRRQLQARTIEIEGLRGANESLERQILELEERHSAE
  VAGYQDSIGQLESDLRNTKSEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTGGLSISGLNPL
  PNPSYLLPPRILSSTASKVSSAGLSLKKEEEEEEEEASKEVSKKTSKVGEGFEETLGEAVISTKKTGKSA
  TEESTSSSQKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146100
• ORF Size: 1503 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146100.5
• RefSeq Size: 2272 bp
• RefSeq ORF: 1506 bp
• Locus ID: 226180
• UniProt ID: P46660
• Cytogenetics: 19 C3
• MW: 55.4 kDa
• Gene Summary: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges (By similarity).[UniProtKB/Swiss-Prot Function]