Tmprss2 (NM_015775) Mouse Tagged ORF Clone

CAT#: MR207852

Tmprss2 (Myc-DDK-tagged) - Mouse transmembrane protease, serine 2 (Tmprss2)

Product Images

Circular map for MR207852

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmprss2
• Synonyms: D16Ertd61e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207852 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGAGAACCACGGGTATCAGTCTGAGC
  ACATCTGTCCTCCGAGACCACCAGTGGCTCCCAATGGCTACAACTTGTATCCAGCCCAGTACTACCCATC
  TCCAGTGCCTCAGTATGCTCCGAGGATTACAACGCAAGCCTCAACATCTGTCATCCACACACATCCCAAG
  TCCTCAGGAGCACTGTGCACCTCAAAGTCTAAGAAATCGCTGTGTTTAGCCCTCGCCCTGGGCACTGTCC
  TCACGGGAGCTGCTGTGGCTGCTGTCTTGCTTTGGAGGTTCTGGGACAGCAACTGTTCTACGTCTGAGAT
  GGAGTGTGGGTCTTCAGGCACATGCATCAGCTCTTCTCTCTGGTGTGACGGGGTAGCACATTGTCCCAAC
  GGAGAAGATGAGAACCGTTGTGTTCGTCTCTACGGACAAAGCTTCATCCTCCAGGTTTACTCATCTCAGA
  GGAAAGCCTGGTATCCCGTGTGCCAGGATGATTGGAGTGAGAGCTACGGGAGAGCAGCATGTAAAGACAT
  GGGATACAAGAACAATTTTTATTCTAGCCAAGGGATACCAGACCAGAGCGGGGCAACGAGCTTTATGAAG
  CTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAGTGACTCATGTTCATCCCGCA
  TGGTGGTTTCTTTGCGCTGTATAGAATGCGGGGTTCGCTCAGTGAAACGCCAGAGCAGGATTGTGGGTGG
  ATTGAATGCCTCACCAGGAGACTGGCCCTGGCAGGTCAGCCTGCACGTCCAAGGCGTCCACGTCTGCGGA
  GGCTCCATCATCACCCCCGAGTGGATTGTGACGGCCGCCCACTGTGTGGAAGAACCCCTCAGCAGCCCGA
  GGTACTGGACGGCATTTGCGGGAATTCTGAGACAGTCTCTCATGTTCTATGGAAGTAGACACCAGGTAGA
  AAAAGTAATTTCCCATCCAAATTACGACTCTAAGACCAAGAATAACGACATTGCTCTCATGAAGCTGCAG
  ACACCTTTGGCTTTTAATGATCTAGTGAAGCCAGTGTGTCTGCCGAACCCAGGCATGATGCTAGACCTAG
  ACCAGGAATGCTGGATTTCGGGGTGGGGGGCCACCTATGAGAAAGGGAAGACCTCGGACGTGTTGAATGC
  TGCCATGGTACCCTTGATCGAGCCCTCCAAATGTAATAGTAAATACATATACAACAACCTAATCACACCA
  GCCATGATCTGTGCCGGCTTCCTCCAGGGGTCTGTCGACTCTTGCCAGGGAGACAGTGGAGGGCCGCTGG
  TTACTTTGAAGAATGGGATCTGGTGGCTGATTGGGGACACGAGCTGGGGCTCGGGCTGTGCCAAGGCACT
  CAGACCTGGAGTATACGGGAACGTGACGGTATTTACAGATTGGATCTACCAGCAAATGAGGGCGAACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207852 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALNSGSPPGIGPCYENHGYQSEHICPPRPPVAPNGYNLYPAQYYPSPVPQYAPRITTQASTSVIHTHPK
  SSGALCTSKSKKSLCLALALGTVLTGAAVAAVLLWRFWDSNCSTSEMECGSSGTCISSSLWCDGVAHCPN
  GEDENRCVRLYGQSFILQVYSSQRKAWYPVCQDDWSESYGRAACKDMGYKNNFYSSQGIPDQSGATSFMK
  LNVSSGNVDLYKKLYHSDSCSSRMVVSLRCIECGVRSVKRQSRIVGGLNASPGDWPWQVSLHVQGVHVCG
  GSIITPEWIVTAAHCVEEPLSSPRYWTAFAGILRQSLMFYGSRHQVEKVISHPNYDSKTKNNDIALMKLQ
  TPLAFNDLVKPVCLPNPGMMLDLDQECWISGWGATYEKGKTSDVLNAAMVPLIEPSKCNSKYIYNNLITP
  AMICAGFLQGSVDSCQGDSGGPLVTLKNGIWWLIGDTSWGSGCAKALRPGVYGNVTVFTDWIYQQMRANS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015775
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015775.2, NP_056590.2
• RefSeq Size: 3175 bp
• RefSeq ORF: 1473 bp
• Locus ID: 50528
• UniProt ID: Q9JIQ8
• Cytogenetics: 16 57.53 cM
• MW: 53.5 kDa
• Gene Summary: Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. The spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.[UniProtKB/Swiss-Prot Function]