Fgb (NM_181849) Mouse Tagged ORF Clone
CAT#: MR207713
- TrueORF®
Fgb (Myc-DDK-tagged) - Mouse fibrinogen beta chain (Fgb)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_181849" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Fgb |
Synonyms | 2510049G14Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207713 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCATCTATGGCTGCTGCTGCTATTGTGTGTTTTCTCAGTTCAAACTCAAGCTGCCGATGATGACT ACGATGAACCGACGGATAGCTTGGATGCCCGTGGTCATCGACCTGTTGACAGGAGAAAGGAAGAGCCTCC TAGCCTAAGACCTGCCCCTCCTCCTATCAGTGGAGGGGGCTATCGAGCCCGTCCAGCCAAGGCTACTGCC AACCAGAAGAAAGTAGAACGGAGACCCCCCGATGCAGGAGGCTGTCTTCATGCTGACACAGACATGGGAG TGTTGTGTCCTACGGGATGTACATTGCAACAAACGTTGTTAAACCAGGAAAGGCCAATCAAGAGTAGTAT TGCTGAGTTAAATAACAACATACAGTCTGTTTCCGATACCTCCTCAGTCACCTTTCAGTACCTGACTCTG CTAAAGGACATGTGGAAAAAGAAGCAAGCTCAAGTTAAAGAGAATGAAAATGTCATAAATGAGTACTCCT CCATCCTGGAAGATCAGAGATTATATATTGATGAGACTGTGAATGATAATATCCCCCTTAATCTTCGTGT GCTCCGCTCAATCCTGGAGGACCTGAGAAGCAAAATACAAAAATTAGAGTCTGACATCTCAGCTCAAATG GAATACTGCCGCACTCCATGCACTGTCAGCTGCAACATTCCAGTGGTGTCTGGGAAAGAGTGTGAGGAGA TCATTAGGAAGGGAGGTGAGACATCCGAAATGTATCTCATCCAGCCTGACACCTCCATCAAGCCGTACAG AGTATACTGTGACATGAAAACAGAAAACGGAGGATGGACAGTCATACAGAACCGTCAGGATGGCAGCGTC GACTTTGGCAGGAAATGGGACCCATACAAAAAAGGATTCGGAAATATTGCCACCAATGAAGACGCGAAGA AGTACTGTGGCTTGCCAGGTGAATATTGGCTTGGCAATGATAAGATTAGCCAGCTCACCAGGATGGGACC CACAGAACTTCTCATTGAAATGGAGGACTGGAAAGGAGACAAGGTGAAGGCACATTATGGAGGCTTCACG GTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCAACGCCCTCA TGGACGGGGCCTCTCAACTGGTGGGGGAGAACAGAACCATGACCATCCACAACGGCATGTTCTTCAGCAC GTACGACAGGGACAACGACGGCTGGGTAACTACGGATCCAAGAAAACAGTGCTCCAAAGAAGATGGTGGT GGCTGGTGGTATAACAGATGCCATGCAGCCAATCCAAATGGCAGATACTACTGGGGTGGCCTTTACAGCT GGGACATGTCCAAGCACGGCACAGATGATGGTGTGGTGTGGATGAACTGGAAGGGATCGTGGTACTCAAT GAGGAGGATGTCTATGAAGATCAGGCCCTTCTTCCCACAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207713 protein sequence
Red=Cloning site Green=Tags(s) MRHLWLLLLLCVFSVQTQAADDDYDEPTDSLDARGHRPVDRRKEEPPSLRPAPPPISGGGYRARPAKATA NQKKVERRPPDAGGCLHADTDMGVLCPTGCTLQQTLLNQERPIKSSIAELNNNIQSVSDTSSVTFQYLTL LKDMWKKKQAQVKENENVINEYSSILEDQRLYIDETVNDNIPLNLRVLRSILEDLRSKIQKLESDISAQM EYCRTPCTVSCNIPVVSGKECEEIIRKGGETSEMYLIQPDTSIKPYRVYCDMKTENGGWTVIQNRQDGSV DFGRKWDPYKKGFGNIATNEDAKKYCGLPGEYWLGNDKISQLTRMGPTELLIEMEDWKGDKVKAHYGGFT VQNEASKYQVSVNKYKGTAGNALMDGASQLVGENRTMTIHNGMFFSTYDRDNDGWVTTDPRKQCSKEDGG GWWYNRCHAANPNGRYYWGGLYSWDMSKHGTDDGVVWMNWKGSWYSMRRMSMKIRPFFPQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181849 |
ORF Size | 1446 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181849.3 |
RefSeq Size | 1575 bp |
RefSeq ORF | 1446 bp |
Locus ID | 110135 |
UniProt ID | Q8K0E8 |
Cytogenetics | 3 36.98 cM |
MW | 54.8 kDa |
Gene Summary | This gene encodes the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded preproprotein is proteolytically processed by thrombin to release an N-terminal fibrinopeptide during the conversion of fibrinogen to insoluble fibrin polymer. The encoded protein interacts with the amyloid beta peptide to form fibrin clots of abnormal structure, and may play an important role in Alzheimer's disease. This gene is located adjacent to the genes encoding fibrinogen alpha and gamma subunits on chromosome 3. [provided by RefSeq, Nov 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201455 | Fgb (untagged) - Mouse fibrinogen beta chain (Fgb), (10ug) |
USD 457.00 |
|
MG207713 | Fgb (tGFP-tagged) - Mouse fibrinogen, B beta polypeptide (Fgb) |
USD 657.00 |
|
MR207713L3 | Lenti ORF clone of Fgb (Myc-DDK-tagged) - Mouse fibrinogen beta chain (Fgb) |
USD 757.00 |
|
MR207713L4 | Lenti ORF clone of Fgb (mGFP-tagged) - Mouse fibrinogen beta chain (Fgb) |
USD 757.00 |
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