Sec61a1 (NM_016906) Mouse Tagged Lenti ORF Clone
CAT#: MR207628L3
- LentiORF®
Lenti ORF clone of Sec61a1 (Myc-DDK-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1)
"NM_016906" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Sec61a1 |
Synonyms | AA408394; AA410007; Sec61a |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR207628).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_016906 |
ORF Size | 1431 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016906.2, NP_058602.1 |
RefSeq Size | 3039 bp |
RefSeq ORF | 1431 bp |
Locus ID | 53421 |
UniProt ID | P61620 |
Cytogenetics | 6 D1 |
Gene Summary | Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (By similarity). Plays a critical role in nephrogenesis, specifically at pronephros stage (PubMed:27392076).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216417 | Sec61a1 (untagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1), (10ug) |
USD 503.00 |
|
MG207628 | Sec61a1 (tGFP-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) |
USD 657.00 |
|
MR207628 | Sec61a1 (Myc-DDK-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) |
USD 457.00 |
|
MR207628L4 | Lenti ORF clone of Sec61a1 (mGFP-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) |
USD 757.00 |
{0} Product Review(s)
Be the first one to submit a review