Atg4d (NM_153583) Mouse Tagged ORF Clone

CAT#: MR207595

Atg4d (Myc-DDK-tagged) - Mouse autophagy-related 4D (yeast) (Atg4d)

Product Images

Circular map for MR207595

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atg4d
• Synonyms: 9830134P12Rik; APG4-D; Apg4d; Atg4dl; Autl4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207595 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCGTGTCGCCGGCTGCGGCGCAGTACCGCAGCGGGAGCTCGGAGGACGCGCGCCGGGCCGATT
  GTCGCCGGCCGAGGGGACAAACCCGTATCCCGGATCCTAGCAACCTGGGACCTTCGGGGTCCGGAGTGGC
  AGCTCTCGGTTCATCTGGGACAGACCCCGCCGAGCCCGACGAGGTGGACAAGTTCAAAGCCAAGTTCCTG
  ACAGCCTGGAACAACGTCAAGTATGGTTGGGCAGTTAAAAGCCGGACCAGCTTCAGCAAGATCTCCACTG
  TTCACCTCTGCGGGCGATGCTACCATTTCGAGGGAGAGGGTGACATCCAGCAGTTTCAAAGGGACTTTGT
  ATCTCGACTATGGCTCACATATCGCCGGGACTTCCCACCCCTTGCTGGAGGCAGCCTGACCTCAGACTGT
  GGCTGGGGATGTATGTTACGAAGTGGTCAGATGATGCTGGCCCAGGGCCTGCTCCTGCATTTTCTGCCTA
  GAGACTGGAGGTGGGTGGAAGGCACAGGCCTGGCTTCCTCTGAGATGCCAGGGCCAGCCTCTCCCAGTCG
  CTGCCGAGGGCCTGGTCGTCGTGGGCCCCCAAGATGGACACAGGGTGCACTGGAGATGGAGCAGGACCGT
  TGGCACCGGCGGATTGTTTCCTGGTTCGCTGATCATCCACGGGCCCCCTTCGGTCTACATCGGCTAGTGG
  AGCTTGGGCGGAGCTCGGGCAAGAAGGCAGGAGACTGGTACGGGCCCTCTGTGGTGGCACACATCCTCAG
  GAAAGCTGTGGAGAGTTGCTCAGAAGTCAGCCGCCTGGTGGTGTACGTCTCTCAGGACTGCACAGTGTAC
  AAGGCAGATGTAGCACGCCTGCTGTCCTGGCCAGATCCTACAGCGGAGTGGAAATCTGTGGTCATCCTGG
  TGCCTGTACGACTGGGCGGGGAGACCCTTAACCCTGTGTACGTGCCCTGTGTGAAGGAGCTTCTGCGTTC
  AGAACTCTGTCTGGGCATCATGGGCGGCAAGCCCCGGCATTCACTGTACTTCATTGGCTATCAGGATGAC
  TTTCTGCTCTACCTGGATCCTCACTACTGCCAGCCCACAGTGGATGTCAGCCAGCCCAGCTTCCCCCTGG
  AGTCCTTTCACTGCACCTCGCCCCGCAAGATGGCCTTTGCCAAGATGGACCCCAGCTGTACCGTGGGGTT
  CTATGCTGGGAACAGGAAGGAGTTTGAGACACTCTGCTCGGAGCTGATGAGGATCCTCAGCTCCTCCTCA
  GTCACGGAACGGTACCCCATGTTCACAGTGGCTGAAGGCCACGCTCAGGACCACAGCCTGGATGCCCTCT
  GCACCCAGCTGTCCCAGCCCACACTGCGGCTACCTTGCACAGGACGACTACTCAAGGCCAAAAGGCCCAG
  CTCCGAGGACTTTGTGTTTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207595 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNSVSPAAAQYRSGSSEDARRADCRRPRGQTRIPDPSNLGPSGSGVAALGSSGTDPAEPDEVDKFKAKFL
  TAWNNVKYGWAVKSRTSFSKISTVHLCGRCYHFEGEGDIQQFQRDFVSRLWLTYRRDFPPLAGGSLTSDC
  GWGCMLRSGQMMLAQGLLLHFLPRDWRWVEGTGLASSEMPGPASPSRCRGPGRRGPPRWTQGALEMEQDR
  WHRRIVSWFADHPRAPFGLHRLVELGRSSGKKAGDWYGPSVVAHILRKAVESCSEVSRLVVYVSQDCTVY
  KADVARLLSWPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRSELCLGIMGGKPRHSLYFIGYQDD
  FLLYLDPHYCQPTVDVSQPSFPLESFHCTSPRKMAFAKMDPSCTVGFYAGNRKEFETLCSELMRILSSSS
  VTERYPMFTVAEGHAQDHSLDALCTQLSQPTLRLPCTGRLLKAKRPSSEDFVFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153583
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153583.7
• RefSeq Size: 3024 bp
• RefSeq ORF: 1425 bp
• Locus ID: 235040
• UniProt ID: Q8BGV9
• Cytogenetics: 9 A3
• MW: 52.9 kDa
• Gene Summary: Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.[UniProtKB/Swiss-Prot Function]