Pik3r3 (NM_181585) Mouse Tagged ORF Clone
CAT#: MR207362
- TrueORF®
Pik3r3 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_181585" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pik3r3 |
Synonyms | AA414954; p55pik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207362 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAATACGGTGTGGAGTATGGACCGCGATGACGCAGACTGGAGGGAGGTGATGATGCCCTATTCGA CAGAACTGATATTTTATATTGAAATGGATCCTCCAGCTCTTCCACCAAAGCCACCTAAGCCAATGACTCC AGCAGTCACAAATGGAATGAAGGACAGTTTCATTTCTCTTCAAGATGCAGAGTGGTACTGGGGAGATATT TCCAGGGAAGAGGTAAATGACAAATTGCGGGACATGCCAGATGGTACCTTCTTAGTTCGTGATGCCTCAA CGAAAATGCAGGGGGATTATACATTGACTTTGAGGAAGGGAGGAAATAATAAATTAATAAAGATCTATCA TCGGGATGGTAAATATGGCTTCTCTGAGCCCCTGACGTTTACTTCTGTGGTGGAGCTTATTAACCACTAC CACCACGAGTCTCTCGCTCAGTACAATCCCAAACTCGACGTGAAGCTGACGTACCCAGTATCCAGATTCC AACAGGATCAGTTGGTAAAAGAAGATAACATTGATGCAGTAGGTAAAAATCTGCAGGAGTTCCACTCTCA GTATCAGGAGAAGAGCAAAGAGTATGACAGGCTGTATGAAGAGTACACAAGGACATCACAGGAAATACAA ATGAAGAGGACTGCCATTGAAGCCTTTAATGAAACAATTAAAATATTTGAGGAGCAGTGTCATACCCAAG AACAACACAGTAAAGACTATATCGAGCGCTTTCGCAGAGAGGGGAATGAGAAGGAGATCGAGCGAATTAT GATGAATTATGATAAATTGAAATCACGTCTTGGTGAGATTCATGATAGCAAACTGCGTCTTGAGCAGGAC TTGAAGAAACAAGCTTTGGACAACCGGGAAATAGATAAAAAAATGAATAGCATCAAACCCGACTTGATCC AGCTGCGTAAGATCCGGGATCAGCACCTTGTATGGCTCAATCACAGAGGAGTGAGGCAGAGGCGCCTGAA TGCCTGGCTGGGGATCAAGAATGAGGACTCAGATGAAAGCTATTTTATCAATGAGGAAGATGAGAACCTG CCGCATTATGATGAGAAAACCTGGTTTGTGGAGGATATCAACCGAGTACAAGCAGAGGACTTGCTTTATG GGAAACCAGATGGTGCATTCTTAATTCGTGAGAGTAGCAAGAAAGGATGTTACGCTTGTTCTGTGGTTGC AGACGGGGAAGTGAAGCACTGTGTCATCTACAGCACGGCTCGAGGATATGGCTTTGCAGAACCCTACAAC CTGTACAGCTCACTGAAGGAGCTGGTGCTCCATTACCAGCAGACATCCCTGGTTCAGCACAACGACTCCC TCAACGTCAGGCTCGCCTACCCTGTCCATGCACAGATGCCTACGCTCTGCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207362 protein sequence
Red=Cloning site Green=Tags(s) MYNTVWSMDRDDADWREVMMPYSTELIFYIEMDPPALPPKPPKPMTPAVTNGMKDSFISLQDAEWYWGDI SREEVNDKLRDMPDGTFLVRDASTKMQGDYTLTLRKGGNNKLIKIYHRDGKYGFSEPLTFTSVVELINHY HHESLAQYNPKLDVKLTYPVSRFQQDQLVKEDNIDAVGKNLQEFHSQYQEKSKEYDRLYEEYTRTSQEIQ MKRTAIEAFNETIKIFEEQCHTQEQHSKDYIERFRREGNEKEIERIMMNYDKLKSRLGEIHDSKLRLEQD LKKQALDNREIDKKMNSIKPDLIQLRKIRDQHLVWLNHRGVRQRRLNAWLGIKNEDSDESYFINEEDENL PHYDEKTWFVEDINRVQAEDLLYGKPDGAFLIRESSKKGCYACSVVADGEVKHCVIYSTARGYGFAEPYN LYSSLKELVLHYQQTSLVQHNDSLNVRLAYPVHAQMPTLCR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181585 |
ORF Size | 1386 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181585.6 |
RefSeq Size | 5004 bp |
RefSeq ORF | 1386 bp |
Locus ID | 18710 |
UniProt ID | Q64143 |
Cytogenetics | 4 D1 |
MW | 54.5 kDa |
Gene Summary | Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG207362 | Pik3r3 (tGFP-tagged) - Mouse phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3) |
USD 657.00 |
|
MR207362L3 | Lenti ORF clone of Pik3r3 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3) |
USD 757.00 |
|
MR207362L4 | Lenti ORF clone of Pik3r3 (mGFP-tagged) - Mouse phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3) |
USD 757.00 |
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