Irak4 (NM_029926) Mouse Tagged ORF Clone
CAT#: MR207322
- TrueORF®
Irak4 (Myc-DDK-tagged) - Mouse interleukin-1 receptor-associated kinase 4 (Irak4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_029926" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Irak4 |
Synonyms | 8430405M07Rik; 9330209D03Rik; IRAK-4; NY-REN-64 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207322 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAAGCCGTTGACACCATCGACATACATACGCAACCTTAATGTGGGGATCCTTAGGAAGCTGTCGG ATTTTATTGATCCTCAAGAAGGGTGGAAGAAATTAGCAGTAGCTATCAAAAAGCCGTCCGGCGACGACAG ATACAATCAGTTCCATATAAGGAGATTCGAAGCCTTACTTCAGACCGGGAAGAGCCCCACCTGTGAACTG CTGTTTGACTGGGGCACCACGAACTGCACAGTTGGCGACCTTGTGGATCTACTGGTCCAGATTGAGCTGT TTGCCCCCGCCACTCTCCTGCTGCCGGATGCCGTTCCCCAAACCGTCAAAAGCCTGCCTCCTAGAGAAGC GGCAACAGTGGCACAAACACACGGGCCTTGTCAGGAAAAGGACAGGACATCCGTAATGCCTATGCCGAAG CTAGAACACAGCTGCGAGCCACCGGACTCCTCAAGCCCAGACAACAGAAGTGTAGAGTCCAGCGACACTC GGTTCCACAGCTTCTCGTTCCATGAACTGAAGAGCATCACAAACAACTTCGACGAGCAACCCGCGTCTGC CGGTGGCAACCGGATGGGAGAGGGGGGATTTGGAGTGGTGTACAAGGGCTGTGTGAACAACACCATCGTG GCGGTGAAGAAGCTCGGAGCGATGGTTGAAATCAGTACTGAAGAACTAAAGCAACAGTTTGATCAAGAAA TTAAAGTAATGGCAACGTGTCAGCACGAGAACCTGGTGGAGCTGCTCGGCTTCTCCAGCGACAGCGACAA CCTGTGCTTAGTGTATGCTTACATGCCCAACGGGTCCTTGCTGGACAGACTGTCCTGCCTGGATGGTACA CCACCGCTTTCCTGGCACACAAGGTGCAAGGTTGCTCAGGGGACAGCAAATGGCATCAGGTTTCTGCATG AAAATCATCACATTCATAGAGATATTAAAAGTGCAAATATCTTACTAGACAGAGACTTTACTGCCAAAAT ATCTGACTTTGGGCTTGCACGGGCTTCGGCAAGGCTAGCGCAGACGGTCATGACCAGCCGAATCGTGGGC ACAACGGCTTACATGGCACCCGAAGCTTTGCGGGGAGAAATAACACCCAAATCTGACATCTACAGCTTCG GCGTGGTTCTGTTGGAGCTGATAACCGGGCTGGCGGCTGTGGATGAAAACCGTGAACCTCAACTACTGCT GGATATTAAAGAAGAGATTGAAGATGAAGAGAAGACGATTGAAGATTACACGGATGAGAAGATGAGCGAT GCGGACCCTGCTTCGGTGGAAGCAATGTACTCTGCTGCTAGCCAGTGTCTGCATGAGAAGAAAAACAGAC GGCCAGACATTGCAAAGGTTCAACAGCTGCTACAAGAGATGTCTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207322 protein sequence
Red=Cloning site Green=Tags(s) MNKPLTPSTYIRNLNVGILRKLSDFIDPQEGWKKLAVAIKKPSGDDRYNQFHIRRFEALLQTGKSPTCEL LFDWGTTNCTVGDLVDLLVQIELFAPATLLLPDAVPQTVKSLPPREAATVAQTHGPCQEKDRTSVMPMPK LEHSCEPPDSSSPDNRSVESSDTRFHSFSFHELKSITNNFDEQPASAGGNRMGEGGFGVVYKGCVNNTIV AVKKLGAMVEISTEELKQQFDQEIKVMATCQHENLVELLGFSSDSDNLCLVYAYMPNGSLLDRLSCLDGT PPLSWHTRCKVAQGTANGIRFLHENHHIHRDIKSANILLDRDFTAKISDFGLARASARLAQTVMTSRIVG TTAYMAPEALRGEITPKSDIYSFGVVLLELITGLAAVDENREPQLLLDIKEEIEDEEKTIEDYTDEKMSD ADPASVEAMYSAASQCLHEKKNRRPDIAKVQQLLQEMSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_029926 |
ORF Size | 1380 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029926.2, NM_029926.3, NM_029926.4, NM_029926.5, NP_084202.2 |
RefSeq Size | 2825 bp |
RefSeq ORF | 1380 bp |
Locus ID | 266632 |
UniProt ID | Q8R4K2 |
Cytogenetics | 15 E3 |
MW | 50.9 kDa |
Gene Summary | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG207322 | Irak4 (tGFP-tagged) - Mouse interleukin-1 receptor-associated kinase 4 (Irak4) |
USD 657.00 |
|
MR207322L3 | Lenti ORF clone of Irak4 (Myc-DDK-tagged) - Mouse interleukin-1 receptor-associated kinase 4 (Irak4) |
USD 757.00 |
|
MR207322L4 | Lenti ORF clone of Irak4 (mGFP-tagged) - Mouse interleukin-1 receptor-associated kinase 4 (Irak4) |
USD 757.00 |
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