Taf1a (NM_021466) Mouse Tagged ORF Clone
CAT#: MR207231
- TrueORF®
Taf1a (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A (Taf1a)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_021466" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Taf1a |
Synonyms | mTAFI48; TAFI48 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207231 representing NM_021466
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGTGATTTCGGTGAAGAGTTGACAAAGCTGGCTGTGGCAGAGGATAACCCAGAAACGTCGGTGC TCTCTAAAACGGGAATGCATTTCCCATGGCTTCACAAGCACGTTGAAGCTGTAGTGACTGGAGGGAAGAA GAGGAAGGATTTTGCTCAGACGACAAGTGCTTGTTTAAGCTTTATCCAGGAAGCCCTGCTGAAGCACCAG TGGCAGCAAGCTGCAGAGTATATGCACAGTTACCTGCAGACCCTGGAGGACTCAGACACCGACAAGAGAC AGGCTGCCCCTGAGATCATTTGGAAGCTTGGAAGTGAAATTCTATTTTACCACCCCAAAAGCAATGTGGA GACTTTTAATAGCTTCGCTGACCGGATGAAAAATATTGGTGTCTTGAATTATTTAAAGATCTCCTTACAA CACGCCTTGTATCTTTTACATCATGGAATGCTTGATGATGCGAACAGGAACCTGAGTAAGGCAGAGACGT GGAGGTATGGAGAAAAGTCGTCTTCTCAGGAAGTGTTGATCAACCTTGTTCAGGCTTACAAAGGGCTTCT GCAGTACTACACTTGGACCAGAAAGAAGATGGAGTTGTCAAAGCTTGATGAAGATGATTACGCTTACGCC GCCAAAACCCGAACCATGCTCAGCCAGAGCTGCAAGACGTCTACAAATATCTGTGCACTGGTCAAAACCC CCGGGGTCTGGGATCCTTTTGTGAAGAGTTATGTGGAGATGCTGGAGTTCTATGGTGATCAAGATGGAGC CCGAGAGATGCTCACCAATTATGCATATGATGAGAAGTTCCCGTCGAACCCCAACGCCCACGTCTACTTA TACGAGTTTCTCAAGAGAGAGAAGGCACCGAGGGCGAAGCTCATAAGTGTTCTTAAGATTTTACATGAGA TTGTGCCATCCCATACACTAATGTTAGAATTCCATACATTGCTTAGAAAATCAGACACAGAAGAACACCA GAAACTGGGCTTGTCTGTGTTATTTGAAGTCCTGGACTTCGCTGGATGCAATAAGAACATAACGGCCTGG AAATACTTGGCAAAGTATCTGAAGCAGATATTAGTGGGAAGCCATCACGAGTGGGTTGAAGAAGAGTGGA AGTCTAGGAGGAACTGGTGGCCAGCTTTTCACTTCAGCTTCTTTTGGGCGAAGAGTGACTGGAAGGCAGA CACTGACTTGGCTTGTGAGAAAGCTTTTGTGGCAGGGGTCCTTTTAGGAAAAGGTTGTAAATATTTTCGG TATATTTTAAAACAAGATCATGAAACCTTGAAGAAGAAAATCAAGCGAATGAAGAAGTCTGTGAAAAAAT ACACTATTGTAAATCCAGGTGTTCACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207231 representing NM_021466
Red=Cloning site Green=Tags(s) MMSDFGEELTKLAVAEDNPETSVLSKTGMHFPWLHKHVEAVVTGGKKRKDFAQTTSACLSFIQEALLKHQ WQQAAEYMHSYLQTLEDSDTDKRQAAPEIIWKLGSEILFYHPKSNVETFNSFADRMKNIGVLNYLKISLQ HALYLLHHGMLDDANRNLSKAETWRYGEKSSSQEVLINLVQAYKGLLQYYTWTRKKMELSKLDEDDYAYA AKTRTMLSQSCKTSTNICALVKTPGVWDPFVKSYVEMLEFYGDQDGAREMLTNYAYDEKFPSNPNAHVYL YEFLKREKAPRAKLISVLKILHEIVPSHTLMLEFHTLLRKSDTEEHQKLGLSVLFEVLDFAGCNKNITAW KYLAKYLKQILVGSHHEWVEEEWKSRRNWWPAFHFSFFWAKSDWKADTDLACEKAFVAGVLLGKGCKYFR YILKQDHETLKKKIKRMKKSVKKYTIVNPGVHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021466 |
ORF Size | 1359 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021466.3, NP_067441.1 |
RefSeq Size | 2430 bp |
RefSeq ORF | 1362 bp |
Locus ID | 21339 |
UniProt ID | P97357 |
Cytogenetics | 1 H5 |
MW | 52.7 kDa |
Gene Summary | Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215966 | Taf1a (untagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A (Taf1a), (10ug) |
USD 503.00 |
|
MR207231L3 | Lenti ORF clone of Taf1a (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A (Taf1a) |
USD 757.00 |
|
MR207231L4 | Lenti ORF clone of Taf1a (mGFP-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A (Taf1a) |
USD 757.00 |
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