Taf1a (NM_021466) Mouse Tagged ORF Clone

CAT#: MR207231

Taf1a (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, A (Taf1a)

Product Images

Circular map for MR207231

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taf1a
• Synonyms: mTAFI48; TAFI48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207231 representing NM_021466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGTGATTTCGGTGAAGAGTTGACAAAGCTGGCTGTGGCAGAGGATAACCCAGAAACGTCGGTGC
  TCTCTAAAACGGGAATGCATTTCCCATGGCTTCACAAGCACGTTGAAGCTGTAGTGACTGGAGGGAAGAA
  GAGGAAGGATTTTGCTCAGACGACAAGTGCTTGTTTAAGCTTTATCCAGGAAGCCCTGCTGAAGCACCAG
  TGGCAGCAAGCTGCAGAGTATATGCACAGTTACCTGCAGACCCTGGAGGACTCAGACACCGACAAGAGAC
  AGGCTGCCCCTGAGATCATTTGGAAGCTTGGAAGTGAAATTCTATTTTACCACCCCAAAAGCAATGTGGA
  GACTTTTAATAGCTTCGCTGACCGGATGAAAAATATTGGTGTCTTGAATTATTTAAAGATCTCCTTACAA
  CACGCCTTGTATCTTTTACATCATGGAATGCTTGATGATGCGAACAGGAACCTGAGTAAGGCAGAGACGT
  GGAGGTATGGAGAAAAGTCGTCTTCTCAGGAAGTGTTGATCAACCTTGTTCAGGCTTACAAAGGGCTTCT
  GCAGTACTACACTTGGACCAGAAAGAAGATGGAGTTGTCAAAGCTTGATGAAGATGATTACGCTTACGCC
  GCCAAAACCCGAACCATGCTCAGCCAGAGCTGCAAGACGTCTACAAATATCTGTGCACTGGTCAAAACCC
  CCGGGGTCTGGGATCCTTTTGTGAAGAGTTATGTGGAGATGCTGGAGTTCTATGGTGATCAAGATGGAGC
  CCGAGAGATGCTCACCAATTATGCATATGATGAGAAGTTCCCGTCGAACCCCAACGCCCACGTCTACTTA
  TACGAGTTTCTCAAGAGAGAGAAGGCACCGAGGGCGAAGCTCATAAGTGTTCTTAAGATTTTACATGAGA
  TTGTGCCATCCCATACACTAATGTTAGAATTCCATACATTGCTTAGAAAATCAGACACAGAAGAACACCA
  GAAACTGGGCTTGTCTGTGTTATTTGAAGTCCTGGACTTCGCTGGATGCAATAAGAACATAACGGCCTGG
  AAATACTTGGCAAAGTATCTGAAGCAGATATTAGTGGGAAGCCATCACGAGTGGGTTGAAGAAGAGTGGA
  AGTCTAGGAGGAACTGGTGGCCAGCTTTTCACTTCAGCTTCTTTTGGGCGAAGAGTGACTGGAAGGCAGA
  CACTGACTTGGCTTGTGAGAAAGCTTTTGTGGCAGGGGTCCTTTTAGGAAAAGGTTGTAAATATTTTCGG
  TATATTTTAAAACAAGATCATGAAACCTTGAAGAAGAAAATCAAGCGAATGAAGAAGTCTGTGAAAAAAT
  ACACTATTGTAAATCCAGGTGTTCACACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207231 representing NM_021466
  Red=Cloning site Green=Tags(s)
  
  MMSDFGEELTKLAVAEDNPETSVLSKTGMHFPWLHKHVEAVVTGGKKRKDFAQTTSACLSFIQEALLKHQ
  WQQAAEYMHSYLQTLEDSDTDKRQAAPEIIWKLGSEILFYHPKSNVETFNSFADRMKNIGVLNYLKISLQ
  HALYLLHHGMLDDANRNLSKAETWRYGEKSSSQEVLINLVQAYKGLLQYYTWTRKKMELSKLDEDDYAYA
  AKTRTMLSQSCKTSTNICALVKTPGVWDPFVKSYVEMLEFYGDQDGAREMLTNYAYDEKFPSNPNAHVYL
  YEFLKREKAPRAKLISVLKILHEIVPSHTLMLEFHTLLRKSDTEEHQKLGLSVLFEVLDFAGCNKNITAW
  KYLAKYLKQILVGSHHEWVEEEWKSRRNWWPAFHFSFFWAKSDWKADTDLACEKAFVAGVLLGKGCKYFR
  YILKQDHETLKKKIKRMKKSVKKYTIVNPGVHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021466
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021466.3, NP_067441.1
• RefSeq Size: 2430 bp
• RefSeq ORF: 1362 bp
• Locus ID: 21339
• UniProt ID: P97357
• Cytogenetics: 1 H5
• MW: 52.7 kDa
• Gene Summary: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits (By similarity).[UniProtKB/Swiss-Prot Function]