Tubg1 (NM_134024) Mouse Tagged ORF Clone
CAT#: MR207207
- TrueORF®
Tubg1 (Myc-DDK-tagged) - Mouse tubulin, gamma 1 (Tubg1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_134024" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tubg1 |
Synonyms | 1500010O08Rik; AI451582; AI503389; Tubg |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207207 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGAGAAATCATCACCCTACAGTTGGGCCAGTGCGGCAATCAGATTGGGTTCGAGTTCTGGAAAC AGCTATGTGCCGAGCATGGCATCAGTCCCGAGGGCATCGTAGAAGAGTTCGCCACGGAGGGCACTGACCG AAAGGACGTCTTTTTCTACCAGGCAGATGATGAGCACTACATCCCCCGGGCTGTGCTGCTGGACCTGGAG CCCCGGGTGATCCATTCCATCCTAAACTCCTCCTATGCTAAGCTCTACAACCCGGAGAACATCTACCTGT CGGAGCATGGCGGAGGAGCTGGCAACAACTGGGCCAGCGGATTCTCCCAGGGAGAAAAAATCCACGAGGA CATTTTTGACATCATAGATCGGGAGGCAGATGGCAGTGACAGTCTAGAGGGATTTGTACTGTGTCATTCC ATTGCTGGGGGGACAGGCTCTGGCTTGGGCTCCTACCTCCTGGAACGGCTAAATGACAGGTACCCCAAAA AACTAGTGCAGACATACTCTGTGTTCCCCAACCAGGATGAGATGAGTGACGTGGTGGTCCAACCCTACAA CTCCCTCCTCACACTAAAGAGGCTGACCCAGAACGCGGACTGTGTGGTGGTGCTGGACAACACGGCCCTG AACCTGATAGCCACAGACCGCCTACACATCCAGAACCCATCCTTCTCCCAGATCAACCAGCTGGTGTCCA CCATCATGTCAGCCAGCACCACCACCCTGCGCTACCCTGGATACATGAACAATGACCTCATCGGCCTCAT CGCCTCGCTCATTCCCACCCCTCGGCTCCACTTCCTCATGACTGGCTACACCCCCCTCACCACGGACCAG TCAGTGGCCAGTGTGAGGAAGACAACAGTCCTGGATGTCATGAGGCGCCTGCTACAGCCCAAGAATGTGA TGGTGTCCACAGGCCGGGATCGTCAGACCAACCACTGCTACATCGCCATTCTCAACATCATCCAGGGAGA GGTGGACCCCACCCAGGTCCACAAGAGCCTGCAGAGGATCCGGGAAAGGAAACTGGCCAACTTCATCCCC TGGGGCCCAGCCAGCATCCAGGTGGCCCTGTCAAGGAAGTCTCCCTACCTGCCCTCAGCCCACCGGGTCA GCGGGCTCATGATGGCCAACCACACCAGTATCTCCTCGCTCTTCGAACGGACCTGTCGCCAGTTTGACAA GCTGCGGAAACGGGAGGCCTTCATGGAACAGTTCCGCAAGGAGGACATCTTCAAGGACAATTTTGACGAG ATGGACACCTCCAGGGAGATTGTGCAGCAGCTGATTGACGAGTACCACGCGGCCACACGGCCAGACTACA TCTCCTGGGGCACCCAGGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207207 protein sequence
Red=Cloning site Green=Tags(s) MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLE PRVIHSILNSSYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHS IAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTAL NLIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQ SVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIP WGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQFDKLRKREAFMEQFRKEDIFKDNFDE MDTSREIVQQLIDEYHAATRPDYISWGTQEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_134024 |
ORF Size | 1356 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134024.1 |
RefSeq Size | 1609 bp |
RefSeq ORF | 1356 bp |
Locus ID | 103733 |
UniProt ID | P83887 |
Cytogenetics | 11 64.24 cM |
MW | 51.1 kDa |
Gene Summary | Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200508 | Tubg1 (untagged) - Mouse tubulin, gamma 1 (Tubg1), (10ug) |
USD 457.00 |
|
MG207207 | Tubg1 (tGFP-tagged) - Mouse tubulin, gamma 1 (Tubg1) |
USD 657.00 |
|
MR207207L3 | Lenti ORF clone of Tubg1 (Myc-DDK-tagged) - Mouse tubulin, gamma 1 (Tubg1) |
USD 757.00 |
|
MR207207L4 | Lenti ORF clone of Tubg1 (mGFP-tagged) - Mouse tubulin, gamma 1 (Tubg1) |
USD 757.00 |
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