Csnk1g1 (BC064645) Mouse Tagged ORF Clone

CAT#: MR206863

Csnk1g1 (Myc-DDK-tagged) - Mouse casein kinase 1, gamma 1 (cDNA clone MGC:67665 IMAGE:5363779)

Product Images

Circular map for MR206863

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csnk1g1
• Synonyms: 9130020E21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206863 representing BC064645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCATTCTAATAGGGAAAAGGATGATAGACAACGGACAACTAAAACCATGGCACAAAGGAATACAC
  ACTGTTCGAGACCATCTGGCACTTCAACATCCTCTGGGGTTCTCATGGTGGGACCCAACTTCAGGGTTGG
  CAAGAAGATAGGGTGTGGGAACTTCGGAGAGCTCAGATTAGGTAAAAATCTCTACACCAATGAATATGTA
  GCCATTAAACTGGAACCAATAAAATCACGTGCTCCACAACTTCATTTAGAGTACAGGTTTTATAAACAGC
  TTGGCAGTGCAGGTGAAGGCCTCCCACAGGTTTATTACTTTGGACCATGTGGGAAGTACAATGCCATGGT
  GCTGGAACTCCTTGGCCCTAGCTTGGAAGACTTGTTTGACCTCTGTGACCGAACGTTTACTTTGAAGACG
  GTGTTAATGATAGCCATCCAGTTGCTTTCTCGAATGGAGTATGTACACTCAAAGAATCTTATTTACCGAG
  ATGTCAAACCAGAGAACTTCTTGATTGGCCGACAAGGCAATAAGAAAGAGCATGTAATACACATTATAGA
  TTTTGGACTGGCCAAGGAATATGTTGATCCTGAAACCAAAAAACACATACCTTACAGAGAGCACAAAAGT
  TTGACTGGAACTGCGAGATACATGTCTATCAACACACATCTAGGCAAAGAGCAAAGCCGGCGAGATGATT
  TGGAAGCCTTAGGCCATATGTTCATGTACTTCCTCCGAGGCAGCCTACCCTGGCAAGGACTCAAGGCTGA
  TACATTAAAAGAGAGATATCAAAAAATCGGTGATACCAAAAGGAATACTCCCATCGAAGCTCTCTGTGAG
  AACTTTCCAGAGGAGATGGCAACCTACCTTCGATATGTCAGGCGATTAGACTTCTTTGAAAAACCTGATT
  ATGAGTATTTACGGACCCTCTTCACAGATCTGTTTGAACGGAAAGGCTATACCTTTGACTATGCCTATGA
  TTGGGTTGGAAGGCCTATTCCTACTCCAGTAGGATCAGTTCATGTAGATTCTGGTGCATCTGCAATAACT
  CGAGAAAGCCACACACACAGGGATCGGCCATCACAACAACAGCCTCTTAGAAATCAGGTGGTTAGCTCAA
  CCAATGGAGAGCTGAATGTCGATGACCCCACTGGAGCTCACTCCAATGCACCAATCACAGCTCATGCCGA
  AGTGGAGGTAGTGGAGGAAGCTAACTGCCTGAAAATGCTCAACCTGTGGTGCTGCTGTTTCTTTAAGAGG
  AAAAGGAAGAAGACAGCCCAGCGACACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206863 representing BC064645
  Red=Cloning site Green=Tags(s)
  
  MDHSNREKDDRQRTTKTMAQRNTHCSRPSGTSTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYV
  AIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKT
  VLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYVDPETKKHIPYREHKS
  LTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCE
  NFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFERKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAIT
  RESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEANCLKMLNLWCCCFFKR
  KRKKTAQRHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC064645
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC064645.1
• RefSeq Size: 5178 bp
• RefSeq ORF: 1292 bp
• Locus ID: 214897
• Cytogenetics: 9 C
• MW: 189.9 kDa
• Gene Summary: Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CLSPN (By similarity). Regulates fast synaptic transmission mediated by glutamate.[UniProtKB/Swiss-Prot Function]