Fbxl2 (NM_178624) Mouse Tagged ORF Clone

CAT#: MR206738

Fbxl2 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 2 (Fbxl2)

Product Images

Circular map for MR206738

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbxl2
• Synonyms: 2810423A21Rik; Fbl3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206738 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCTCAAACAGTGATGATGGCCTTATCAACAAGAAGCTACCCAAGGAGCTCCTCTTGAGAATAT
  TCTCCTTCTTGGACATCGTAACTCTATGCCGATGTGCACAGATCTCCAAGGCCTGGAACATCTTAGCCCT
  GGATGGCAGCAACTGGCAACGGGTGGATCTTTTTAACTTCCAGACAGATGTAGAGGGCCGAGTGGTGGAA
  AACATCTCCAAGAGGTGCGGTGGCTTCCTTAGAAAGCTCAGCCTGCGTGGCTGCATCGGAGTCGGGGACT
  CCTCTTTGAAGACCTTTGCACAGAACTGCCGGAACATTGAACATTTAAACCTCAATGGCTGCACGAAAAT
  CACTGACAGCACGTGTTACAGCCTTAGCAGATTCTGTTCCAAGCTGAAACACCTGGATCTCACGTCCTGC
  GTGTCTGTTACCAACAGCTCTTTAAAGGGCATCAGCGAGGGCTGCCGGAACCTGGAATATCTGAACCTCT
  CCTGGTGTGACCAGATCACAAAGGAAGGCATTGAGGCGCTGGTGCGGGGGTGCCGGGGTTTGAAAGCCCT
  GCTCCTGAGGGGTTGTACACAGTTAGAGGACGAAGCTCTGAAACACATTCAGAACCACTGCCACGAGCTG
  GTGAGCCTCAACCTGCAGTCCTGCTCACGCATCACTGATGATGGCGTGGTGCAGATCTGCAGGGGCTGCC
  ACCGGCTACAGGCTCTGTGCCTCTCGGGTTGTAGCAACCTTACGGATGCATCTCTCACAGCCTTGGGCCT
  GAACTGCCCCAGACTACAAGTTTTGGAGGCTGCCCGGTGCTCCCATCTGACCGACGCAGGCTTTACACTG
  CTAGCTCGGAATTGCCATGAGCTGGAGAAGATGGACCTTGAAGAATGTGTCCTGATTACCGACAGCACCC
  TCGTCCAGCTCTCCATCCACTGTCCCAAGCTGCAAGCCCTGAGCTTGTCCCACTGTGAGCTCATCACAGA
  TGAGGGGATCCTGCACCTGAGCAGCAGCACCTGTGGGCACGAGAGACTCCGGGTGCTGGAGCTGGACAAC
  TGCCTTCTTGTCACGGACGCCTCGCTGGAGCACCTGGAGAACTGCCGAGGCCTGGAGCGACTGGAGCTTT
  ACGACTGCCAGCAGGTCACCCGTGCAGGCATCAAGCGCATGCGGGCTCAGCTTCCTCATGTCAAAGTCCA
  TGCCTACTTTGCTCCAGTCACCCCTCCACCAGCAGTGGCAGGAAGTGGACATCGACTGTGCAGATGCTGT
  GTCATACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206738 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVFSNSDDGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAWNILALDGSNWQRVDLFNFQTDVEGRVVE
  NISKRCGGFLRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCYSLSRFCSKLKHLDLTSC
  VSVTNSSLKGISEGCRNLEYLNLSWCDQITKEGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNHCHEL
  VSLNLQSCSRITDDGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQVLEAARCSHLTDAGFTL
  LARNCHELEKMDLEECVLITDSTLVQLSIHCPKLQALSLSHCELITDEGILHLSSSTCGHERLRVLELDN
  CLLVTDASLEHLENCRGLERLELYDCQQVTRAGIKRMRAQLPHVKVHAYFAPVTPPPAVAGSGHRLCRCC
  VIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178624
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178624.6, NP_848739.1
• RefSeq Size: 3203 bp
• RefSeq ORF: 1272 bp
• Locus ID: 72179
• UniProt ID: Q8BH16
• Cytogenetics: 9 F3
• MW: 46.9 kDa
• Gene Summary: Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (By similarity). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (PubMed:21343341).[UniProtKB/Swiss-Prot Function]