Tdg (NM_011561) Mouse Tagged ORF Clone

CAT#: MR206699

Tdg (Myc-DDK-tagged) - Mouse thymine DNA glycosylase (Tdg), transcript variant 1

Product Images

Circular map for MR206699

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tdg
• Synonyms: E130317C12Rik; JZA-3; Jza1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206699 representing NM_011561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCAGAGGCCGCGCGCAGCTATTCTCTGGAGCAAGTTCAAGCTTTGTATTCATTTCCATTTCAAC
  AAATGATGGCAGAAGTTCCTAACATGGCAGTCACGACTGGACAGCAGGTGCCAGCAGTAGCTCCTAACAT
  GGCAACCGTGACAGAACAGCAGGTGCCCGCAGACGCTCCTGTCCAGGAACCTGCACCAGAAGCTCCAAAG
  AGAAGGAAAAGGAAACCCAGAGCAGCAGAGCCCCAGGAACCAGTGGAGCCCAAAAAACCTGCTACGTCGA
  AGAAATCCGGCAAGTCTACAAAATCAAAGGAAAAGCAGGAGAAAATCACAGACGCGTTTAAAGTGAAAAG
  GAAAGTGGACCGCTTCAACGGCGTCTCTGAAGCTGAGCTTCTGACCAAGACTCTTCCTGACATTTTGACC
  TTCAATCTGGATATTGTGATCATTGGCATTAACCCGGGATTAATGGCTGCTTACAAAGGACATCACTACC
  CTGGGCCTGGAAATCACTTCTGGAAGTGTCTGTTCATGTCGGGGCTGAGTGAGGTGCAGCTGAATCACAT
  GGATGACCACACCTTACCCGGCAAGTACGGCATCGGATTCACCAACATGGTGGAACGGACGACGCCGGGC
  AGCAAGGATCTGTCTAGTAAAGAGTTCCGGGAAGGAGGGCGCATCCTGGTGCAGAAACTGCAGAAATATC
  AGCCACGAATAGCGGTGTTTAATGGAAAATGTATTTATGAAATTTTCAGTAAAGAAGTTTTTGGAGTAAA
  GGTTAAGAACTTGGAATTTGGGCTTCAACCCCACAAGATCCCAGACACAGAAACTCTGTGCTACGTCATG
  CCGTCGTCCAGCGCCAGATGTGCTCAGTTTCCCCGGGCCCAGGACAAAGTTCATTACTACATTAAGCTGA
  AGGACTTGAGAGACCAGCTGAAAGGCATTGAACGCAACACCGACGTTCAGGAAGTGCAGTATACATTTGA
  CCTGCAGCTTGCGCAAGAGGACGCAAAGAAGATGGCTGTTAAGGAAGAAAAGTATGATCCAGGCTATGAG
  GCAGCTTACGGCGGCGCCTATGGGGAAAACCCATGTAATGGGGAACCTTGTGGCATTGCTTCAAATGGGC
  TAACAGCCCACAGTGCGGAGCCGAGAGGAGAAGCGACCCCCGGCGATGTTCCAAATGGGCAGTGGATGGC
  ACAGTCGTTTGCAGAGCAGATCCCTTCTTTTAATAATTGTGGGACCCGAGAGCAGGAAGAAGAGAGCCAC
  GCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206699 representing NM_011561
  Red=Cloning site Green=Tags(s)
  
  MDAEAARSYSLEQVQALYSFPFQQMMAEVPNMAVTTGQQVPAVAPNMATVTEQQVPADAPVQEPAPEAPK
  RRKRKPRAAEPQEPVEPKKPATSKKSGKSTKSKEKQEKITDAFKVKRKVDRFNGVSEAELLTKTLPDILT
  FNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPG
  SKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVM
  PSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNTDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYE
  AAYGGAYGENPCNGEPCGIASNGLTAHSAEPRGEATPGDVPNGQWMAQSFAEQIPSFNNCGTREQEEESH
  A
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011561
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3119 bp
• RefSeq ORF: 1194 bp
• Locus ID: 21665
• UniProt ID: P56581
• Cytogenetics: 10 39.72 cM
• MW: 44.6 kDa
• Gene Summary: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine (PubMed:21817016). Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC (PubMed:21722948). Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.[UniProtKB/Swiss-Prot Function]