Adrb2 (NM_007420) Mouse Tagged ORF Clone

CAT#: MR206632

Adrb2 (Myc-DDK-tagged) - Mouse adrenergic receptor, beta 2 (Adrb2)

Product Images

Circular map for MR206632

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adrb2
• Synonyms: Adrb; Adrb-2; Bad; Badm; Gpc; Gpcr7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206632 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCACACGGGAACGACAGCGACTTCTTGCTGGCACCCAACGGAAGCCGAGCGCCAGACCACGACG
  TCACTCAGGAACGGGACGAAGCGTGGGTTGTGGGCATGGCCATCCTCATGTCGGTTATCGTCCTGGCCAT
  CGTGTTTGGCAACGTGCTGGTCATCACAGCCATTGCCAAGTTCGAGCGACTACAAACCGTCACCAACTAC
  TTCATAATCTCCTTGGCGTGTGCTGATCTAGTCATGGGCCTAGCGGTGGTGCCGTTTGGGGCCAGTCACA
  TCCTTATGAAAATGTGGAATTTTGGCAACTTCTGGTGCGAGTTCTGGACTTCCATTGATGTGTTGTGCGT
  CACAGCCAGCATCGAGACCCTGTGCGTGATTGCAGTGGATCGCTATGTTGCTATCACATCGCCCTTCAAG
  TACCAGAGCCTGCTGACCAAGAATAAGGCCCGAGTGGTCATCCTGATGGTATGGATTGTATCTGGCCTTA
  CCTCCTTTTTGCCTATCCAGATGCACTGGTACCGTGCCACCCACAAGAAAGCTATCGATTGTTACACCGA
  GGAGACTTGCTGTGACTTCTTCACGAACCAGGCCTACGCCATCGCGTCCTCGATTGTGTCTTTCTACGTG
  CCCCTGGTGGTGATGGTCTTTGTCTATTCCCGGGTCTTCCAGGTGGCCAAAAGGCAGCTGCAGAAGATAG
  ACAAATCTGAAGGAAGATTCCACGCCCAAAACCTCAGCCAGGTGGAGCAGGATGGGCGGAGCGGCCACGG
  ACTCCGAAGGTCCTCCAAGTTCTGCTTGAAAGAGCACAAAGCCCTCAAGACTTTAGGCATCATCATGGGC
  ACATTCACCCTCTGCTGGCTGCCCTTCTTCATTGTCAATATCGTGCACGTTATCAGGGACAACCTCATCC
  CTAAGGAAGTTTACATCCTCCTTAACTGGTTGGGCTACGTCAACTCTGCCTTCAATCCTCTTATCTACTG
  TCGGAGTCCAGATTTCAGGATTGCCTTTCAAGAGCTTCTGTGCCTTCGCAGGTCTTCTTCGAAAACCTAT
  GGGAACGGCTACTCTAGCAATAGCAACGGCAGAACGGACTACACAGGGGAGCCAAACACTTGTCAGCTGG
  GGCAGGAGAGAGAACAGGAACTGCTGTGTGAGGATCCCCCAGGCATGGAAGGCTTTGTGAACTGTCAAGG
  TACTGTGCCTAGCCTTAGCGTTGACTCCCAAGGAAGGAACTGTAGTACAAATGACTCGCCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206632 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPHGNDSDFLLAPNGSRAPDHDVTQERDEAWVVGMAILMSVIVLAIVFGNVLVITAIAKFERLQTVTNY
  FIISLACADLVMGLAVVPFGASHILMKMWNFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYVAITSPFK
  YQSLLTKNKARVVILMVWIVSGLTSFLPIQMHWYRATHKKAIDCYTEETCCDFFTNQAYAIASSIVSFYV
  PLVVMVFVYSRVFQVAKRQLQKIDKSEGRFHAQNLSQVEQDGRSGHGLRRSSKFCLKEHKALKTLGIIMG
  TFTLCWLPFFIVNIVHVIRDNLIPKEVYILLNWLGYVNSAFNPLIYCRSPDFRIAFQELLCLRRSSSKTY
  GNGYSSNSNGRTDYTGEPNTCQLGQEREQELLCEDPPGMEGFVNCQGTVPSLSVDSQGRNCSTNDSPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007420
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007420.3
• RefSeq Size: 2269 bp
• RefSeq ORF: 1257 bp
• Locus ID: 11555
• UniProt ID: P18762
• Cytogenetics: 18 35.1 cM
• MW: 47 kDa
• Gene Summary: This intronless gene belongs to the G-protein-coupled receptor superfamily, which includes transmembrane proteins that play a role in signal transduction across biological membranes resulting in a variety of physiological responses. The encoded protein is a beta-2 adrenergic receptor which is activated by catecholamine ligands such as adrenaline and epinephrine. The protein participates in the classical signaling pathway involving G protein, adenylyl cyclase, cAMP (3'-5'-cyclic adenosine monophosphate) and protein kinase A (PKA). In humans, this gene is implicated in susceptibility to nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Apr 2013]