Calr (NM_007591) Mouse Tagged ORF Clone
CAT#: MR206584
- TrueORF®
Calr (Myc-DDK-tagged) - Mouse calreticulin (Calr)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_007591" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Calr |
Synonyms | Calregulin; CRT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206584 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTTTCGGTGCCGCTCCTGCTTGGCCTCCTCGGCCTGGCCGCCGCAGACCCTGCCATCTATTTCA AAGAGCAGTTCTTGGACGGAGATGCCTGGACCAACCGCTGGGTCGAATCCAAACATAAGTCCGATTTTGG CAAATTTGTCCTCAGTTCTGGCAAATTTTACGGGGACCTGGAGAAGGATAAAGGGCTGCAGACAAGCCAA GATGCCCGATTTTACGCACTGTCCGCCAAATTCGAACCCTTCAGCAATAAGGGCCAGACACTGGTGGTAC AGTTCACGGTGAAGCATGAGCAGAATATCGACTGTGGGGGCGGCTACGTGAAGCTGTTTCCGAGTGGCTT GGACCAGAAGGACATGCATGGAGACTCAGAATATAACATCATGTTTGGTCCGGACATCTGCGGTCCTGGC ACCAAGAAGGTTCATGTCATCTTTAACTACAAGGGCAAGAATGTGCTGATCAACAAGGATATCCGGTGTA AGGATGATGAATTCACACACCTATACACACTGATTGTGCGGCCAGACAACACCTATGAGGTGAAAATTGA CAACAGCCAGGTGGAGTCAGGCTCCTTGGAGGATGATTGGGACTTTCTGCCACCCAAGAAGATAAAGGAC CCTGATGCTGCCAAGCCGGAAGACTGGGATGAACGAGCCAAGATCGATGACCCCACAGATTCCAAGCCTG AGGACTGGGACAAGCCAGAGCACATCCCTGACCCTGATGCTAAGAAGCCTGAGGACTGGGATGAAGAGAT GGATGGAGAGTGGGAACCACCAGTGATTCAAAATCCTGAATACAAGGGCGAGTGGAAACCACGTCAAATT GACAACCCAGATTACAAGGGTACCTGGATACACCCAGAAATTGACAACCCTGAATACTCCCCCGATGCAA ATATCTATGCCTATGATAGTTTTGCTGTACTGGGCCTAGATCTCTGGCAGGTCAAGTCCGGGACAATCTT TGACAATTTCCTCATCACCAATGATGAGGCCTATGCAGAGGAGTTTGGCAATGAGACGTGGGGTGTTACC AAGGCTGCAGAGAAGCAGATGAAGGACAAGCAGGATGAGGAGCAGAGGCTTAAGGAAGAAGAAGAGGACA AGAAGCGTAAAGAGGAAGAAGAAGCTGAGGATAAAGAGGATGATGATGACAGAGATGAAGATGAGGATGA AGAAGATGAGAAGGAGGAAGATGAGGAAGAATCCCCTGGCCAAGCCAAGGATGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206584 protein sequence
Red=Cloning site Green=Tags(s) MLLSVPLLLGLLGLAAADPAIYFKEQFLDGDAWTNRWVESKHKSDFGKFVLSSGKFYGDLEKDKGLQTSQ DARFYALSAKFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPSGLDQKDMHGDSEYNIMFGPDICGPG TKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFLPPKKIKD PDAAKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPRQI DNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVT KAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDDDRDEDEDEEDEKEEDEEESPGQAKDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007591 |
ORF Size | 1251 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007591.1 |
RefSeq Size | 1943 bp |
RefSeq ORF | 1251 bp |
Locus ID | 12317 |
UniProt ID | P14211 |
Cytogenetics | 8 41.21 cM |
MW | 48 kDa |
Gene Summary | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208210 | Calr (untagged) - Mouse calreticulin (Calr), (10ug) |
USD 457.00 |
|
MG206584 | Calr (tGFP-tagged) - Mouse calreticulin (Calr) |
USD 657.00 |
|
MR206584L3 | Lenti ORF clone of Calr (Myc-DDK-tagged) - Mouse calreticulin (Calr) |
USD 757.00 |
|
MR206584L4 | Lenti ORF clone of Calr (mGFP-tagged) - Mouse calreticulin (Calr) |
USD 757.00 |
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