Vmp1 (NM_029478) Mouse Tagged ORF Clone

CAT#: MR206378

Vmp1 (Myc-DDK-tagged) - Mouse vacuole membrane protein 1 (Vmp1)

Product Images

Circular map for MR206378

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vmp1
• Synonyms: 3110098I04Rik; 4930579A11Rik; AI787464; mir-21a; ni-2; Tango5; Tmem49
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206378 representing NM_029478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGAATGGAAAAAATTGTGACCAGAGACGCATAGCAATGAGTAAGGATCAGCACAATGGAAGTC
  TCACAGACCCCTCTTCAGTTCATGAGAAGAAGAGAAGGGATCGGGAAGAAAGACAGAATATTGTCCTGTG
  GAGACAGCCACTCATTACCTTGCAGTATTTCTCTCTGGAAACTCTTGTAGTTTTGAAGGAATGGACCTCA
  AAATTGTGGCATCGTCAAAGCATTGTGGTGTCCTTTTTACTGCTGCTTGCTGCGCTTGTAGCTACGTATT
  ATGTGGAAGGAGCGCACCAACAGTATGTGCAGCGGATAGAGAAGCAGTTTCTTTTGTATGCATACTGGAT
  AGGCCTGGGGATTTTGTCCTCTGTTGGTCTTGGAACAGGACTGCACACCTTTCTGCTTTATCTGGGCCCA
  CATATAGCTTCAGTTACATTAGCTGCTTATGAATGCAATTCGGTGAATTTCCCTGAGCCACCCTATCCTG
  ACCAGATTATCTGCCCAGAGGAAGAAGGCGCTGAGGGAGCCATTTCTTTGTGGAGTATCATCTCAAAAGT
  TAGAATTGAAGCCTGCATGTGGGGCATTGGAACAGCCATTGGAGAGCTGCCTCCATATTTCATGGCCAGG
  GCAGCTCGCCTCTCAGGTGCTGAACCAGATGATGAAGAGTATCAGGAATTTGAAGAAATGCTGGAACATG
  CAGAGGCTGCACAAGACTTTGCATCACGGGCTAAACTGGCAGTTCAAAAACTAGTACAGAAAGTTGGATT
  TTTTGGAATTTTGGCCTGTGCTTCTATTCCAAACCCCCTGTTTGACCTGGCTGGAATAACGTGTGGGCAC
  TTCCTTGTACCTTTCTGGACCTTCTTTGGTGCAACCCTGATTGGGAAAGCAATCATTAAAATGCATATCC
  AGAAAATATTTGTTATAGTAACTTTCAGCAAGCACATCGTGGAGCAGATGGTGACTTTCATTGGTGCTGT
  CCCCGGCATAGGTCCGTCTCTGCAGAAGCCTTTTCAAGAGTACCTGGAGGCGCAGCGGCAGAAGCTTCAT
  CACAGAAGTGAAGCGGGCACACCGCAGGGAGAAAACTGGTTATCCTGGATGTTTGAGAAGCTGGTGGTTG
  CAATGGTGTGTTACTTTGTCCTGTCTATTATTAACTCCATGGCACAAAACTATGCCAAACGAATCCAGCA
  GCGCTTGAACTCAGAGGAGAAAACTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206378 representing NM_029478
  Red=Cloning site Green=Tags(s)
  
  MAENGKNCDQRRIAMSKDQHNGSLTDPSSVHEKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTS
  KLWHRQSIVVSFLLLLAALVATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGP
  HIASVTLAAYECNSVNFPEPPYPDQIICPEEEGAEGAISLWSIISKVRIEACMWGIGTAIGELPPYFMAR
  AARLSGAEPDDEEYQEFEEMLEHAEAAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGH
  FLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSKHIVEQMVTFIGAVPGIGPSLQKPFQEYLEAQRQKLH
  HRSEAGTPQGENWLSWMFEKLVVAMVCYFVLSIINSMAQNYAKRIQQRLNSEEKTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029478
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029478.6
• RefSeq Size: 2773 bp
• RefSeq ORF: 1221 bp
• Locus ID: 75909
• UniProt ID: Q99KU0
• Cytogenetics: 11 C
• MW: 46.4 kDa
• Gene Summary: Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death (By similarity). May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis (PubMed:17940279). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions. Plays a role in the initial stages of the autophagic process through its interaction with BECN1. Required for autophagosome formation (By similarity).[UniProtKB/Swiss-Prot Function]