Ifit3 (NM_010501) Mouse Tagged Lenti ORF Clone
CAT#: MR206340L4
- LentiORF®
Lenti ORF clone of Ifit3 (mGFP-tagged) - Mouse interferon-induced protein with tetratricopeptide repeats 3 (Ifit3)
"NM_010501" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Ifit3 |
Synonyms | Ifi49; P49 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR206340).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010501 |
ORF Size | 1212 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010501.1, NP_034631.1 |
RefSeq Size | 1998 bp |
RefSeq ORF | 1212 bp |
Locus ID | 15959 |
UniProt ID | Q64345 |
Cytogenetics | 19 C1 |
Gene Summary | IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205676 | Ifit3 (untagged) - Mouse interferon-induced protein with tetratricopeptide repeats 3 (Ifit3), (10ug) |
USD 457.00 |
|
MG206340 | Ifit3 (tGFP-tagged) - Mouse interferon-induced protein with tetratricopeptide repeats 3 (Ifit3) |
USD 657.00 |
|
MR206340 | Ifit3 (Myc-DDK-tagged) - Mouse interferon-induced protein with tetratricopeptide repeats 3 (Ifit3) |
USD 457.00 |
|
MR206340L3 | Lenti ORF clone of Ifit3 (Myc-DDK-tagged) - Mouse interferon-induced protein with tetratricopeptide repeats 3 (Ifit3) |
USD 757.00 |
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