Ss18l1 (NM_178750) Mouse Tagged Lenti ORF Clone
CAT#: MR206309L3
- LentiORF®
Lenti ORF clone of Ss18l1 (Myc-DDK-tagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1)
"NM_178750" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ss18l1 |
Synonyms | A230053O16Rik; CREST |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR206309).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_178750 |
ORF Size | 1209 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178750.4, NP_848865.3 |
RefSeq Size | 4395 bp |
RefSeq ORF | 1209 bp |
Locus ID | 269397 |
UniProt ID | Q8BW22 |
Cytogenetics | 2 H4 |
Gene Summary | Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203600 | Ss18l1 (untagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1), (10ug) |
USD 457.00 |
|
MG206309 | Ss18l1 (tGFP-tagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1) |
USD 657.00 |
|
MR206309 | Ss18l1 (Myc-DDK-tagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1) |
USD 457.00 |
|
MR206309L4 | Lenti ORF clone of Ss18l1 (mGFP-tagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1) |
USD 757.00 |
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