Ncf1 (NM_010876) Mouse Tagged ORF Clone

CAT#: MR206087

Ncf1 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 1 (Ncf1)

Product Images

Circular map for MR206087

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncf1
• Synonyms: Ncf-1; NCF-47K; NOXO2; p47-phox; p47; p47phox
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206087 representing NM_010876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACACCTTCATTCGCCATATTGCCCTGCTGGGCTTCGAGAAGCGCTTCATCCCCAGCCAGCACT
  ATGTGTACATGTTCCTGGTTAAGTGGCAGGACCTGTCGGAGAAGGTGGTCTACAGAAAATTCACCGAGAT
  CTACGAGTTCCATAAAATGCTGAAGGAGATGTTCCCCATTGAGGCCGGCGAGATCCACACAGAGAACAGA
  GTCATCCCACACCTCCCGGCACCCAGGTGGTTTGATGGGCAACGAGCCGCTGAGAGTCGCCAGGGCACTC
  TCACTGAATACTTCAACGGCCTCATGGGACTGCCCGTGAAGATCTCCCGCTGCCCACACCTGCTGGACTT
  CTTCAAAGTGCGGCCTGATGACCTGAAACTGCCCACTGACAGCCAGGCGAAGAAGCCTGAGACATACCTG
  GTGCCCAAAGATGGCAAGAATAACGTAGCTGACATCACAGGCCCCATCATCCTTCAGACCTATCGGGCCA
  TTGCTGACTACGAGAAGAGTTCGGGAACAGAGATGACCGTGGCAACCGGAGACGTGGTGGACGTTGTGGA
  GAAGAGCGAGAGCGGCTGGTGGTTTTGCCAGATGAAGACAAAGCGAGGTTGGGTCCCTGCATCCTATCTG
  GAGCCCCTTGACAGTCCCGACGAGGCGGAGGATCCGGATCCCAACTACGCAGGTGAACCGTATGTAACCA
  TCAAAGCGTACGCTGCTGTTGAAGAGGACGAGATGTCCCTGTCTGAGGGTGAAGCCATTGAGGTCATTCA
  TAAGCTCCTGGATGGCTGGTGGGTGGTCAGGAAAGGGGATATCACCGGCTATTTCCCATCCATGTATCTG
  CAGAAGGCTGGGGAGGAGATAACCCAGGCCCAGCGACAGATCAGAGGCCGCGGGGCACCACCTCGAAGGT
  CGACCATCCGCAACGCACAGAGCATCCACCAGCGTTCTCGGAAGCGCCTTAGCCAGGACACCTATCGCCG
  CAACAGCGTCCGATTCCTGCAGCAGCGCAGACGCCCGGGGCGACCCGGGCCGCTGAGCACGGATGGCACA
  AAGGACAATCCATCGACTCCGCGCGTCAAACCACAGCCCGCGGTGCCTCCGCGACCCAGCTCGGACCTCA
  TCCTGCACCGCTGCACAGAGAGCACCAAACGGAAGCTGACGTCCGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010876
• ORF Size: 1170 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010876.4
• RefSeq Size: 2721 bp
• RefSeq ORF: 1173 bp
• Locus ID: 17969
• UniProt ID: Q09014
• Cytogenetics: 5 74.47 cM
• MW: 45.1 kDa
• Gene Summary: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).[UniProtKB/Swiss-Prot Function]