Fbxo4 (NM_134099) Mouse Tagged ORF Clone

CAT#: MR206003

Fbxo4 (Myc-DDK-tagged) - Mouse F-box protein 4 (Fbxo4)

Product Images

Circular map for MR206003

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbxo4
• Synonyms: 1700096C12Rik; AI851261; AW494535; Fbx4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206003 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAAGCGAGCCCCGCGGAGCCGGCTCCCCGCCGCCCGCCAGCGACTGGGGCCGCCTGGAGGCAG
  CCATCCTGAGCGGCTGGAGGACCTTCTGGTATTCGGTGGCCAAGGAGCGGGCGACGCCGACGGCCTCTCG
  GAAGGAGGCGGCGGAGGAGACGAGCGCGCTGACGCGGCTGCCGGTTGATGTGCAGTTGTATATCTTGTCG
  TTTCTTTCACCCCACGATCTGTGCCAGCTGGGAAGTACAGATCATTACTGGAACAAAACTGTAAGAGACC
  CAATTCTCTGGAGATACTTCCTGTTGCGGGATCTCCCTTCTTGGTCTTCGGTTGATTGGAAGTCACTTCC
  AGATCTAGAGATCTTAAAAAAGCCAATATCTGAGGTCACCGACAGCACTTGTCTTGATTACATGGAGGTT
  TATAAAATGTGCTGTCCATATACGCGAAGAGCCTTGAAAGCCAGCCGTCCTATGTATGGAGTGGTTACCT
  CTTTCTTACACTCACTGATCATTCAGAATGAACCCCGGTTTGCTATGTTTGGACCAGGTTTGGAAGAACT
  GAACACATCCTTGGTGTTGAGTTTGATGTCTTCTGAGGACCTTTGCCCAACTGCTGGTTTACCTCACAGA
  CAGATTGATGGTATTGGATCTGGAGTCAACTTCCAGTTGAACAACCAGCAAAAATTCAACATCCTGATAT
  TATACTCGACTACCAGAAAAGAAAGAGACAGAGCAAGGGAGGAGCACACCAGCACCGTTAACAAGATGTT
  CAGCCTACAGAGTGAGGGGGACGAGCAGCAGGGCAGCCGCTACAGTGTGATCCCGCAGATTCAGAAAGTG
  TGTGAAGTCGTAGACGGGTTCATCTACGTGGCAAACGCTGAAGCTCACCGACGTCATGAATGGCAAGATG
  AATTTTCTCGGATTATGGCCATGACAGACCCAGCTTTTGGATCTTCAGGAAGACCCATGCTGGTTTTATC
  TTGTATTTCTCAAGCAGATGTAAAGAGAATGCCTTGTTTTTATTTAGCTCATGAGCTGCGCCTCAGTCTT
  CTAAACCACCCATGGATGGTCCAGGATACAGAGGCTGAAACTCTGACTGGTTTTTTGAATGGCATTGAGT
  GGATTCTTGAAGAAGTAGAATCTAAGCGTGCAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206003 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGSEPRGAGSPPPASDWGRLEAAILSGWRTFWYSVAKERATPTASRKEAAEETSALTRLPVDVQLYILS
  FLSPHDLCQLGSTDHYWNKTVRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDSTCLDYMEV
  YKMCCPYTRRALKASRPMYGVVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEDLCPTAGLPHR
  QIDGIGSGVNFQLNNQQKFNILILYSTTRKERDRAREEHTSTVNKMFSLQSEGDEQQGSRYSVIPQIQKV
  CEVVDGFIYVANAEAHRRHEWQDEFSRIMAMTDPAFGSSGRPMLVLSCISQADVKRMPCFYLAHELRLSL
  LNHPWMVQDTEAETLTGFLNGIEWILEEVESKRAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134099
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134099.1, NP_598860.1
• RefSeq Size: 3479 bp
• RefSeq ORF: 1158 bp
• Locus ID: 106052
• UniProt ID: Q8CHQ0
• Cytogenetics: 15 A1
• MW: 43.8 kDa
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1 (By similarity).[UniProtKB/Swiss-Prot Function]