Hdac8 (NM_027382) Mouse Tagged ORF Clone
CAT#: MR205884
- TrueORF®
Hdac8 (Myc-DDK-tagged) - Mouse histone deacetylase 8 (Hdac8)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_027382" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hdac8 |
Synonyms | 2610007D20Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205884 representing NM_027382
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGCCAGAGGAACCCGCCAACAGTGGGCATTCACTACCCCCGGTTTATATTTACAGTCCCGAGT ATGTCAGCATCTGCGACTCCCTTGTGAAGGTCCCCAAACGGGCCAGTATGGTCCACTCTCTGATCGAAGC CTATGCCCTGCATAAACAAATGAGGATAGTGAAGCCCAAAGTGGCCTCCATGGAGGAGATGGCCACCTTC CACACTGATGCCTATCTGCAACATCTCCAGAAAGTCAGCCAAGAAGGTGATGAGGACCATCCAGACTCCA TAGAATATGGACTAGGTTATGACTGCCCAGCCACAGAAGGGATATTTGACTATGCAGCAGCTATAGGAGG AGGTACAATCACAGCTGCCCAGTGCCTGATTGACGGGAAGTGTAAAGTAGCCATCAACTGGTCTGGAGGG TGGCATCATGCAAAGAAAGATGAAGCATCTGGTTTCTGTTATCTCAATGATGCTGTCCTGGGAATATTAC GATTGCGACGGAAATTTGACCGTATTCTCTACGTGGATTTGGATCTACACCATGGAGATGGTGTAGAAGA TGCCTTCAGTTTTACATCGAAGGTTATGACTGTGTCCCTGCACAAGTTCTCCCCAGGATTTTTCCCAGGA ACAGGTGACATGTCTGATGTTGGCCTGGGGAAAGGACGGTACTACAGTGTCAATGTGCCCATTCAGGATG GCATCCAGGATGAGAAGTACTATCACATCTGTGAAAGTGTACTAAAAGAAGTGTACCAGGCCTTCAATCC GAAGGCAGTGGTTTTACAGCTGGGAGCAGATACTATTGCCGGAGATCCAATGTGTTCCTTTAATATGACA CCAGTGGGGATCGGCAAGTGTCTGAAGTATGTCCTACAGTGGCAGCTGGCAACTCTGATTTTAGGAGGAG GAGGCTATAACCTTGCCAACACGGCTCGTTGCTGGACATACTTGACCGGGGTCATCCTAGGGAAAACGCT ATCCTCTGAGATCCCAGATCATGAGTTTTTCACAGCATATGGTCCTGATTATGTGCTGGAAATCACGCCA AGCTGCCGGCCAGACCGCAATGAGCCCCACCGAATCCAGCAAATCCTCAACTACATCAAAGGGAATCTGA AGCATGTGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205884 representing NM_027382
Red=Cloning site Green=Tags(s) MEMPEEPANSGHSLPPVYIYSPEYVSICDSLVKVPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATF HTDAYLQHLQKVSQEGDEDHPDSIEYGLGYDCPATEGIFDYAAAIGGGTITAAQCLIDGKCKVAINWSGG WHHAKKDEASGFCYLNDAVLGILRLRRKFDRILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPG TGDMSDVGLGKGRYYSVNVPIQDGIQDEKYYHICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMT PVGIGKCLKYVLQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITP SCRPDRNEPHRIQQILNYIKGNLKHVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_027382 |
ORF Size | 1131 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027382.4 |
RefSeq Size | 1724 bp |
RefSeq ORF | 1134 bp |
Locus ID | 70315 |
UniProt ID | Q8VH37 |
Cytogenetics | X D |
MW | 42.2 kDa |
Gene Summary | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205016 | Hdac8 (untagged) - Mouse histone deacetylase 8 (Hdac8), (10ug) |
USD 686.00 |
|
MG205884 | Hdac8 (tGFP-tagged) - Mouse histone deacetylase 8 (Hdac8) |
USD 886.00 |
|
MR205884L3 | Lenti ORF clone of Hdac8 (Myc-DDK-tagged) - Mouse histone deacetylase 8 (Hdac8) |
USD 986.00 |
|
MR205884L4 | Lenti ORF clone of Hdac8 (mGFP-tagged) - Mouse histone deacetylase 8 (Hdac8) |
USD 986.00 |
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