St8sia2 (NM_009181) Mouse Tagged ORF Clone

CAT#: MR205823

St8sia2 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2 (St8sia2)

Product Images

Circular map for MR205823

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: St8sia2
• Synonyms: AI323367; Siat8b; ST8SiaII; STX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205823 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTGCAGTTCCGGAGCTGGATGCTGGCCGCGCTTACGCTGCTCGTGGTCTTCCTCATCTTCGCAG
  ACATCTCAGAGATCGAAGAAGAAATCGGGAATTCTGGAGGCAGAGGTACAATCAGATCAGCTGTGAACAG
  CTTACATAGCAAATCTAATAGAGCTGAAGTTGTAATAAATGGCTCTTCACCGCCAGCTGTCGCTGACAGA
  AGTAATGAAAGCCTTAAGCACAACATCCAGCCAGCCTCATCCAAATGGAGACACAACCAGACGCTCTCTC
  TGAGGATCAGGAAGCAAATTTTAAAGTTCTTGGATGCGGAGAAGGATATTTCTGTCCTTAAGGGGACCCT
  GAAGCCTGGAGACATTATTCATTATATCTTTGATCGAGACAGCACAATGAACGTGTCCCAGAACCTCTAT
  GAACTCCTCCCCAGAACCTCACCTCTGAAGAATAAGCATTTCCAGACTTGTGCCATCGTGGGCAACTCGG
  GGGTCTTGCTGAACAGCGGCTGTGGCCAGGAGATTGACACACACAGCTTTGTCATAAGGTGCAACCTGGC
  TCCAGTTCAGGAGTATGCCCGGGATGTGGGCCTCAAGACAGACCTAGTGACCATGAACCCCTCTGTCATC
  CAGCGGGCCTTTGAGGACCTTGTGAACGCCACGTGGCGGGAGAAGCTGCTGCAGCGGCTGCACGGCCTCA
  ATGGGAGCATCCTGTGGATCCCTGCCTTCATGGCCCGGGGTGGCAAGGAGCGTGTGGAGTGGGTCAATGC
  TCTCATCCTGAAGCACCATGTGAACGTGCGCACGGCTTACCCTTCCCTGCGCCTGCTGCACGCAGTCCGA
  GGATATTGGCTGACCAACAAAGTCCACATCAAAAGACCAACCACCGGCCTCTTGATGTACACCCTGGCCA
  CACGCTTCTGCAATCAGATCTACCTTTATGGCTTCTGGCCCTTCCCACTGGATCAGAATCAGAACCCCGT
  CAAGTATCACTATTATGACAGCCTCAAGTATGGCTACACTTCCCAGGCCAGCCCCCACACCATGCCCTTG
  GAATTCAAGGCCCTCAAGAGCCTACATGAACAGGGGGCATTGAAACTGACTGTCGGCCAGTGTGATGGGG
  CTACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205823 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQLQFRSWMLAALTLLVVFLIFADISEIEEEIGNSGGRGTIRSAVNSLHSKSNRAEVVINGSSPPAVADR
  SNESLKHNIQPASSKWRHNQTLSLRIRKQILKFLDAEKDISVLKGTLKPGDIIHYIFDRDSTMNVSQNLY
  ELLPRTSPLKNKHFQTCAIVGNSGVLLNSGCGQEIDTHSFVIRCNLAPVQEYARDVGLKTDLVTMNPSVI
  QRAFEDLVNATWREKLLQRLHGLNGSILWIPAFMARGGKERVEWVNALILKHHVNVRTAYPSLRLLHAVR
  GYWLTNKVHIKRPTTGLLMYTLATRFCNQIYLYGFWPFPLDQNQNPVKYHYYDSLKYGYTSQASPHTMPL
  EFKALKSLHEQGALKLTVGQCDGAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009181
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009181.2, NP_033207.2
• RefSeq Size: 5376 bp
• RefSeq ORF: 1128 bp
• Locus ID: 20450
• UniProt ID: O35696
• Cytogenetics: 7 D1
• MW: 42.4 kDa
• Gene Summary: May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.[UniProtKB/Swiss-Prot Function]