Wnt1 (NM_021279) Mouse Tagged ORF Clone

CAT#: MR205702

Wnt1 (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 1 (Wnt1)

Product Images

Circular map for MR205702

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt1
• Synonyms: Int-1; sw; swaying; Wnt-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205702 representing NM_021279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTCTGGGCGCTGCTGCCCAGCTGGGTTTCTACTACGTTGCTACTGGCACTGACCGCTCTGCCCG
  CAGCCCTAGCTGCCAACAGTAGTGGCCGATGGTGGGGCATCGTGAACATAGCCTCCTCCACGAACCTGTT
  GACGGATTCCAAGAGCCTGCAGCTGGTGCTCGAGCCCAGTCTGCAGCTGCTGAGCCGCAAGCAGCGGCGA
  CTGATCCGACAGAACCCGGGGATCCTGCACAGCGTGAGTGGAGGGCTCCAGAGCGCCGTGCGAGAGTGCA
  AATGGCAATTCCGAAACCGCCGCTGGAACTGCCCCACTGCTCCGGGGCCCCACCTCTTCGGCAAGATCGT
  CAACCGAGGCTGCCGAGAAACAGCGTTCATCTTCGCAATCACCTCCGCCGGGGTCACACATTCCGTGGCG
  CGCTCCTGCTCCGAAGGCTCCATCGAGTCCTGCACCTGCGACTACCGGCGGCGCGGCCCTGGGGGCCCCG
  ACTGGCACTGGGGGGGCTGCAGTGACAACATCGATTTTGGTCGCCTCTTTGGCCGAGAGTTCGTGGACTC
  CGGGGAGAAGGGGCGGGACCTACGCTTCCTCATGAACCTTCACAACAACGAGGCAGGGCGAACGACCGTG
  TTCTCTGAGATGCGCCAAGAGTGCAAATGCCACGGGATGTCCGGCTCCTGCACGGTGCGCACGTGTTGGA
  TGCGGCTGCCCACGCTGCGCGCTGTGGGCGACGTGCTGCGCGACCGCTTCGACGGTGCCTCCCGCGTCCT
  TTACGGCAACAGAGGCAGCAACCGCGCCTCGCGGGCGGAGCTGCTGCGCCTGGAGCCCGAAGACCCCGCG
  CACAAGCCTCCCTCCCCTCACGACCTCGTCTACTTCGAGAAATCGCCCAACTTCTGCACGTACAGTGGCC
  GCCTGGGCACAGCTGGCACAGCTGGACGAGCTTGCAACAGCTCGTCTCCCGCGCTGGACGGCTGTGAGCT
  GCTGTGCTGTGGCCGAGGCCACCGCACGCGCACGCAGCGCGTCACGGAGCGCTGCAACTGCACCTTCCAC
  TGGTGCTGCCACGTCAGCTGCCGCAACTGCACGCACACGCGCGTTCTGCACGAGTGTCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205702 representing NM_021279
  Red=Cloning site Green=Tags(s)
  
  MGLWALLPSWVSTTLLLALTALPAALAANSSGRWWGIVNIASSTNLLTDSKSLQLVLEPSLQLLSRKQRR
  LIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPTAPGPHLFGKIVNRGCRETAFIFAITSAGVTHSVA
  RSCSEGSIESCTCDYRRRGPGGPDWHWGGCSDNIDFGRLFGREFVDSGEKGRDLRFLMNLHNNEAGRTTV
  FSEMRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGSNRASRAELLRLEPEDPA
  HKPPSPHDLVYFEKSPNFCTYSGRLGTAGTAGRACNSSSPALDGCELLCCGRGHRTRTQRVTERCNCTFH
  WCCHVSCRNCTHTRVLHECL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021279
• ORF Size: 1110 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021279.3
• RefSeq Size: 2371 bp
• RefSeq ORF: 1113 bp
• Locus ID: 22408
• UniProt ID: P04426
• Cytogenetics: 15 54.65 cM
• MW: 41.5 kDa
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (By similarity). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (PubMed:15454084, PubMed:16116452). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (PubMed:2202907, PubMed:16116452). Has a role in osteoblast function, bone development and bone homeostasis (By similarity).[UniProtKB/Swiss-Prot Function]