Apoa5 (NM_080434) Mouse Tagged ORF Clone

CAT#: MR205662

Apoa5 (Myc-DDK-tagged) - Mouse apolipoprotein A-V (Apoa5)

Product Images

Circular map for MR205662

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apoa5
• Synonyms: 1300007O05Rik; Apoav; RAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205662 representing NM_080434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGTCATCACTTGGGCCCTCGCCCTCCTCGCAGTGTTCGCAAGCACTCAGGCACGGAAGAGCC
  TCTGGGACTACTTCAGCCAAAACAGTTGGAGCAAAGGCGTGATGGGCCAGCCACAGAAGCTGGCACAGGA
  GAACCTGAAAGGCAGCTTCGAGCAAGACCTCTACAATATGAACAATTACCTAGAAAAGCTGGGACCCTTG
  AGAGGGCCTGGGAAGGAGCCTCCTCTGCTGGCCCAGGACCCAGAAGGCATTCGGAAACAGCTGCAGCAGG
  AGCTGGGGGAAGTGAGCTCGCGCCTGGAGCCCTACATGGCTGCGAAGCACCAGCAGGTAGGCTGGAACTT
  GGAGGGCCTGAGGCAGCAGTTGAAACCCTACACGGCGGAGCTGATGGAGCAGGTGGGCCTGAGTGTGCAG
  GAGCTGCAGGAGCAGCTGCGCGTGGTGGGAGAAGACACCAAGGCTCAGCTCCTGGGGGGCGTGGACGAGG
  CGCTGAACCTGCTGCAGGACATGCAGAGTCGAGTGCTGCACCATACCGACCGAGTCAAAGAGCTCTTCCA
  CCCTTACGCAGAACGCTTGGTGACTGGAATTGGGCACCACGTGCAGGAGCTGCACCGCAGTGTCGCTCCT
  CACGCAGCTGCCAGCCCCGCGCGACTCAGTCGCTGCGTGCAGACTCTGTCCCACAAACTCACACGTAAGG
  CGAAGGACCTGCACACCAGCATCCAACGCAACCTGGACCAGCTGCGCGATGAGCTCAGTGCCTTCATCCG
  CGTCAGCACAGATGGGGCGGAAGATGGAGATTCCCTGGACCCTCAGGCTCTCTCCGAGGAGGTCCGCCAG
  AGACTGCAGGCTTTTCGGCATGACACCTACCTGCAGATTGCTGCATTTACTCAGGCCATTGACCAGGAGA
  CAGAGGAAATTCAGCACCAGCTAGCCCCACCCCCGCCTAGCCACAGCGCCTTCGCTCCTGAGCTGGGACA
  CTCAGACAGTAATAAGGCCCTGAGCAGACTGCAGAGCCGACTGGACGACCTGTGGGAAGATATTGCCTAT
  GGCCTTCAAGACCAGGGTCATAGTCATTTGAGTGACCCTGAGGGTCACTCCGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205662 representing NM_080434
  Red=Cloning site Green=Tags(s)
  
  MAAVITWALALLAVFASTQARKSLWDYFSQNSWSKGVMGQPQKLAQENLKGSFEQDLYNMNNYLEKLGPL
  RGPGKEPPLLAQDPEGIRKQLQQELGEVSSRLEPYMAAKHQQVGWNLEGLRQQLKPYTAELMEQVGLSVQ
  ELQEQLRVVGEDTKAQLLGGVDEALNLLQDMQSRVLHHTDRVKELFHPYAERLVTGIGHHVQELHRSVAP
  HAAASPARLSRCVQTLSHKLTRKAKDLHTSIQRNLDQLRDELSAFIRVSTDGAEDGDSLDPQALSEEVRQ
  RLQAFRHDTYLQIAAFTQAIDQETEEIQHQLAPPPPSHSAFAPELGHSDSNKALSRLQSRLDDLWEDIAY
  GLQDQGHSHLSDPEGHSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080434
• ORF Size: 1104 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080434.4
• RefSeq Size: 2321 bp
• RefSeq ORF: 1107 bp
• Locus ID: 66113
• UniProt ID: Q8C7G5
• Cytogenetics: 9 A5.2
• MW: 41.7 kDa
• Gene Summary: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages (By similarity).[UniProtKB/Swiss-Prot Function]