Amz2 (NM_025275) Mouse Tagged ORF Clone

CAT#: MR205580

Amz2 (Myc-DDK-tagged) - Mouse archaelysin family metallopeptidase 2 (Amz2)

Product Images

Circular map for MR205580

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Amz2
• Synonyms: AA408420; ESTM12; X83328
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205580 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACAAGATGCAGGTACTACGGCACTCCGAGCACACACTGAAAACAGCTCTCCTCTCAAAGAACC
  CAGTGCTTGTGTCACAGTATGAGAAGTTAGATGCTGGGGAGCAACGGTTAATGAATGAAGCCTTCCAGCC
  ACGCAGTAACCTGTTTGAGCCCATTACTGTGCACTCCCAGTCAGACTGGATCTCCTCCCACCCCGAGGCT
  CCCCAAGACTTTGAGCAGTTTTTCTCTGACCGCTACAGAAAGGCACCCTGTCCAAAGAAACATATAATCT
  ACATTCAGTCCATTGGCTCTCTAGGCAACACCAGAGTCATCAGTGAAGAGTATATTAAATGGCTCAAGGG
  CTACTGTGAAGCATTTTTCTACGGTTTGAAGGTGAAGTTCTTAGAACCTGTTTCGGTCTCTGAAACCAAG
  TGTTCCTTTAGAGTGAATGAGCACACACAAAACCTGCAAATCCACACAGGACATATCCTGGCATTCTTGA
  AGAAGAATAAACCGGAAGATGCCTTCTGCATCGTGGGGATCACAATGATCGATCTCTACCCTCGGGACTC
  GTGGAACTTCGTCTTTGGACAGGCCTCCTTGTCAAGTGGTGTGGGCATATTCAGCTTTGCCAGGTATGGC
  AAGGACTTCTACACCTCGAAATACGAAGGCAATGTGACGTCTCTGCAGCTAACGTCTCCCACTGACTACT
  CCATTTTTGATAACTATTACATCCCTGAAATCACTAGTGTTTTGCTGTTGCGATCCTGTAAGACTTTAAC
  CCATGAGATCGGACACATACTTGGACTGCGACACTGCCAGTGGCTTGCGTGTTTAATGCAAGGCTCCAAT
  CACTTGGAGGAATCTGACCGGCGTCCTCTGAATGTTTGCCCTATCTGCTTACGGAAGCTGCAGAGTGCCA
  TTGGCTTCAATATTGTGGAGAGATACAGAGCACTGGTGAAGTGGATTGACGACGAATCTTGCAACGAATC
  AGGAGCAACTCCGAAAAGTAGTTCTGAGCATGCGCATTTGCCGAAACCTGTGGAAGCCTTTAAGGATTGG
  AGAGAGTGGCTAATGAGATGCATTGCTCTCCTTGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205580 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADKMQVLRHSEHTLKTALLSKNPVLVSQYEKLDAGEQRLMNEAFQPRSNLFEPITVHSQSDWISSHPEA
  PQDFEQFFSDRYRKAPCPKKHIIYIQSIGSLGNTRVISEEYIKWLKGYCEAFFYGLKVKFLEPVSVSETK
  CSFRVNEHTQNLQIHTGHILAFLKKNKPEDAFCIVGITMIDLYPRDSWNFVFGQASLSSGVGIFSFARYG
  KDFYTSKYEGNVTSLQLTSPTDYSIFDNYYIPEITSVLLLRSCKTLTHEIGHILGLRHCQWLACLMQGSN
  HLEESDRRPLNVCPICLRKLQSAIGFNIVERYRALVKWIDDESCNESGATPKSSSEHAHLPKPVEAFKDW
  REWLMRCIALLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025275
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025275.3, NM_025275.4, NP_079551.3
• RefSeq Size: 2816 bp
• RefSeq ORF: 1092 bp
• Locus ID: 13929
• UniProt ID: Q400C8
• Cytogenetics: 11 71.89 cM
• MW: 41.8 kDa
• Gene Summary: Zinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze neurogranin nor angiotensin-2 (By similarity).[UniProtKB/Swiss-Prot Function]