Surf6 (NM_009298) Mouse Tagged ORF Clone
CAT#: MR205420
- TrueORF®
Surf6 (Myc-DDK-tagged) - Mouse surfeit gene 6 (Surf6)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_009298" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Surf6 |
Synonyms | D2Wsu129e; Surf-6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205420 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCTCTCCTGGCCAAGGATACCTATCTTCAGGACTTGGCCAAGAAGATCTGCGCGCAGCCTGGCC CTGAACGACAGAGGAGCACATGGGGTGTCAGAACAAAAGGCTCAGAAGCTGCTGGCGCCCCCAAAAAGAA AAGGAAGAAAACACAAAAGAAGTCTCCGGAGCAGGAACAGAAAGCTATGGATCACAAGACTAAAGCCCTG GGCAAGAAGCCTCCTACCTCTTCTAGGCCCAAGAACCCAATGGTATCCAAACAAGAGAAGGGCTTGAGCT CCCTGGGGTCCCCTAAAGATAGCCAAGGCACAGCACGGGAGTCTGTCTTTGCACTGGATTTTCTGCGACA GCGGCTGCATGAAAAGATCCAGCTGGCCCGGGGCCAGGGTAGCACCAAGGAGTTGTCTGCTGCCACTCTC GAGAAAAGACAGCGGAGAAAGCAGGAGAGGGAACGGAAGAAGAGGAAGAGGAAGGAGCGCCAAGCAAAGC AGCAGGTTGCAGAGGCTGAGAAAAAGGAGGAGCCAGTAGAGGTGACCCCCAAAATGGCCTGCAAGGAGCT GCAGGAATCAGGGCTGATCTTTAACAAGGTGGAAGTGACTGAAGAGGAGCCAGCCAGCAAGGCCCAGCGC AAGAAGGAGAAGAGGCAGAAAGTGAAAGGGAACCTGACACCGCTCACAGGCAGGAACTACAGGCAGCTGT TGGACCGTCTGCAGGCACGGCAGGGCCGCCTGGACGAGCTTCGTGATCAGGATGCAGCCAAGGCCCAGGA GCTGGAGGCCAAGATGAAGTGGACCAACTTGCTGTACAAGGCTGAGGGTGTGAAGATCAGGGATGACGAA CGCCTGCTGCAGGAAGCCCTGAAGCGCAAAGAGAAGCGCCGGGCACAGCGGCAGCGCAAGTGGGAGAAGC GTTCAGAGCATGTGGTGGAGAAGATGCAGCAGCGACAGGACAAGCGGCGCCAGAACCTGCGCAAGAAGAA GGCAGCTCGGGCTGAGCGACGGCTACAGAAGGCCCACAAGAAGGGCCGGGTGCTTCCCCAGGACCTGGAG CGTGCAGGCCTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205420 protein sequence
Red=Cloning site Green=Tags(s) MASLLAKDTYLQDLAKKICAQPGPERQRSTWGVRTKGSEAAGAPKKKRKKTQKKSPEQEQKAMDHKTKAL GKKPPTSSRPKNPMVSKQEKGLSSLGSPKDSQGTARESVFALDFLRQRLHEKIQLARGQGSTKELSAATL EKRQRRKQERERKKRKRKERQAKQQVAEAEKKEEPVEVTPKMACKELQESGLIFNKVEVTEEEPASKAQR KKEKRQKVKGNLTPLTGRNYRQLLDRLQARQGRLDELRDQDAAKAQELEAKMKWTNLLYKAEGVKIRDDE RLLQEALKRKEKRRAQRQRKWEKRSEHVVEKMQQRQDKRRQNLRKKKAARAERRLQKAHKKGRVLPQDLE RAGLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009298 |
ORF Size | 1068 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009298.4 |
RefSeq Size | 2593 bp |
RefSeq ORF | 1068 bp |
Locus ID | 20935 |
UniProt ID | P70279 |
Cytogenetics | 2 19.08 cM |
MW | 41.2 kDa |
Gene Summary | Binds to both DNA and RNA in vitro, with a stronger binding capacity for RNA. May represent a nucleolar constitutive protein involved in ribosomal biosynthesis or assembly.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209482 | Surf6 (untagged) - Mouse surfeit gene 6 (Surf6), (10ug) |
USD 503.00 |
|
MG205420 | Surf6 (tGFP-tagged) - Mouse surfeit gene 6 (Surf6) |
USD 657.00 |
|
MR205420L3 | Lenti ORF clone of Surf6 (Myc-DDK-tagged) - Mouse surfeit gene 6 (Surf6) |
USD 757.00 |
|
MR205420L4 | Lenti ORF clone of Surf6 (mGFP-tagged) - Mouse surfeit gene 6 (Surf6) |
USD 757.00 |
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