Nf2 (BC005442) Mouse Tagged Lenti ORF Clone
CAT#: MR205376L4
- LentiORF®
Lenti ORF clone of Nf2 (mGFP-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053)
"BC005442" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Nf2 |
Synonyms | merlin |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR205376).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | BC005442 |
ORF Size | 1059 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC005442, AAH05442 |
RefSeq Size | 2127 bp |
RefSeq ORF | 1061 bp |
Locus ID | 18016 |
Cytogenetics | 11 3.0 cM |
Gene Summary | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217723 | Nf2 (untagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053), (10ug) |
USD 503.00 |
|
MG205376 | Nf2 (tGFP-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053) |
USD 657.00 |
|
MR205376 | Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053) |
USD 457.00 |
|
MR205376L3 | Lenti ORF clone of Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (cDNA clone MGC:6102 IMAGE:3496053) |
USD 757.00 |
{0} Product Review(s)
Be the first one to submit a review