Tex19.1 (NM_028602) Mouse Tagged ORF Clone
CAT#: MR205325
- TrueORF®
Tex19.1 (Myc-DDK-tagged) - Mouse testis expressed gene 19.1 (Tex19.1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_028602" in other vectors (3)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tex19.1 |
Synonyms | 2410081M02Rik; mTex19.1; Tex19; Tex19a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205325 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTCCCCCAGTCAGCGTTCGCCATGGGGCCAGGGGCATGTCCTGCCTCTATGGGGCATGGCTGTACC ATCTTGTCCATGGGGAACAGACGAAGATCTGCTTTGCTTGCTTCAAGGCAGCTTTCCTATTGAATAAACT CTACCTGGAGATGGGAGACTGGCAAGAGGAAGAAGAGGAGGAAGAGGAAGAGGAAGATGCCGATCTCCTG GAATACTTGTCAGAGTCAGAGTCAGATTCTGAGCAGGAGCCAGGGCCTGAGCAGGATGCATGGCGGGGAT TGGGGTCCCTTTATGTGCCACAGAGTGTCTCTGAAGGGTCTGGGGTCCTGCTGCCAACCCCTGTGTGGAC ACAGGGCATACTATTCTCCATTTTTGTGCCCACTGAGCTCTTTCCTCAGGAAGCTGTACCCCTGGATCTG GGTCCTGAGGATGCTGAGTGGACCCAGGCCCTTCCCTGGAGACTTGATGGGCTTTTTCCCTGCTCGCACC AGCTCATCCCTCCTCTGACTTGGTGGGATATTTTTGATGTGATGCCATCTCCTGGGCAACCTGTGTTGTT GGAGTTGAGATGCCACTGGCCCTTGGACCAGACAGTAGCACAATCCTGGTTGCAAGACCAGAAGTTTGTC CTCCTGTTGGATGGCGTCCAATCTAGGTGCCACCTGCTGTCAATGCGTGTCCGCTGGGTCGTAAGGACTC AGGTCCAGCACTGGCAGGTGTTGCTGGACCCTGGTGAGATGTGGGTGGCCCATTTTCGGAAAGAAGTTGG GCAGCACGGCCTGTACCATCAGAGCCTGAATCCCTGGAGGCTGAGCATCCTGACAGCTTCAGAATTAGGG ATGGAGTTATTGCCTGCCACCTGCTACCTGTGGAATAAAGGCTTCTGGGTAGGTTCCTTCTTGCCCTGGC ACATTAACATGCCAGAGACCCGGAGCTGGGAGCCAGGAGAGAGGCTGTTTATCACAGATGCTACTATTTG TGGTACTGACTACCACCTTGCTCAGTCTTTCCTTGATTCCCACCCCACCCCCCACCCCCTCCTGACCCTT ACTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205325 protein sequence
Red=Cloning site Green=Tags(s) MCPPVSVRHGARGMSCLYGAWLYHLVHGEQTKICFACFKAAFLLNKLYLEMGDWQEEEEEEEEEEDADLL EYLSESESDSEQEPGPEQDAWRGLGSLYVPQSVSEGSGVLLPTPVWTQGILFSIFVPTELFPQEAVPLDL GPEDAEWTQALPWRLDGLFPCSHQLIPPLTWWDIFDVMPSPGQPVLLELRCHWPLDQTVAQSWLQDQKFV LLLDGVQSRCHLLSMRVRWVVRTQVQHWQVLLDPGEMWVAHFRKEVGQHGLYHQSLNPWRLSILTASELG MELLPATCYLWNKGFWVGSFLPWHINMPETRSWEPGERLFITDATICGTDYHLAQSFLDSHPTPHPLLTL TP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028602 |
ORF Size | 1059 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028602.2, NP_082878.2 |
RefSeq Size | 1754 bp |
RefSeq ORF | 1056 bp |
Locus ID | 73679 |
UniProt ID | Q99MV2 |
Cytogenetics | 11 E2 |
MW | 40.4 kDa |
Gene Summary | Required during spermatogenesis and placenta development, participating in the repression of retrotransposable elements and preventing their mobilization (PubMed:18802469, PubMed:21103378, PubMed:23364048, PubMed:23674551, PubMed:28254886). With its paralog, Tex19.2, collaborates with the Piwi-interacting RNA (piRNA) pathway, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins. Interacts with Piwi proteins and directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:28254886). Also during spermatogenesis, promotes, with UBR2, SPO11-dependent meiotic recombination (PubMed:28708824). Interacts with LINE-1 retrotransposon encoded LIRE1, stimulates LIRE1 polyubiquitination, mediated by UBR2, and degradation, inhibiting LINE-1 retranstoposon mobilization (PubMed:28806172).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG205325 | Tex19.1 (tGFP-tagged) - Mouse testis expressed gene 19 (Tex19) |
USD 657.00 |
|
MR205325L3 | Lenti ORF clone of Tex19.1 (Myc-DDK-tagged) - Mouse testis expressed gene 19.1 (Tex19.1) |
USD 757.00 |
|
MR205325L4 | Lenti ORF clone of Tex19.1 (mGFP-tagged) - Mouse testis expressed gene 19.1 (Tex19.1) |
USD 757.00 |
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