Catsper3 (BC089518) Mouse Tagged ORF Clone

CAT#: MR205090

Catsper3 (Myc-DDK-tagged) - Mouse cation channel, sperm associated 3 (cDNA clone MGC:107210 IMAGE:6774298)

Product Images

Circular map for MR205090

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Catsper3
• Synonyms: 4921522D01Rik; CACRC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205090 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACCACGGTCACCACCAACTCCTTTTTACTGGTCTTGGGGACTAATTATGACATACAATTCGAGT
  TTTTCAGAACCTTTGAGGTCTCAGAGCTTTTCTTTGTATCTGTCTATGTCTGCGAGTTCCTCATGAAGGT
  CTATGTGGACCCCATTACATACTGGAAGGATGGCTATAACATACTGGATGTGATCATTCTCATCATTCTC
  ACCATACCCTATCTCCTCCGCAAAATCAAGGGGAATCATTCTGCATACCTCCACTTTGCTGATGGCATCC
  AGTCTCTACGAATCCTCAAGCTTATCTCCTACAGTAGGGGCATCAGGACACTCATCATCGCTGTGGGGGA
  GACGGTCTACACTGTGGCCTCGGTGCTGACGCTGCTCTTCCTCCTCATGTTTGTGTTCGCGATCCTGGGA
  TTCTGCCTATTTGGCGTGACGGACAGAGGCGACCTGGAGAACTGGGGGAACCTGGCTTCAGCTTTCTTTA
  CTCTCTTCAGTTTGGCCACGGTTGATGGCTGGACTGACCTGCAGGAAGAGCTGGACAAGAGGAAGTTTAC
  TGTGAGCCGGGCGTTTACTATCCTCTTCATCTTGCTTGCATCCTTCATCTTCCTCAACATGTTTGTGGGT
  GTGATGATCATGCACACGGAGGATTCCATGAAAAAGTTTGAGCGGGATCTGACGTTGGAGAGGAACCTTG
  CGATTATGGAGGAGAAGCAAATAATCCTGAAACGCCAGCAAGAGGAGGTCAACAGGCTGATGAACACACA
  GAAAAGTGGTAGCATGAACTTCATTGATATGGTGGAGGGCTTCAAGAAGACCCTGCGGCACACAGACCCC
  ATGGTTCTGGATGACTTCAGCACTAGTCTCTCCTTCATTGATATCTACTTGGTCACTCTGGACAACCAAG
  ATGTTATTGTCAGCAAGCTTCAGGAGCTCTACTGTGAGATTGTGAACGTGCTGAGCCTGATGTTGGAAGA
  CATGCCCAAGGAGAGCTCGTCCAGCCTCTCGGGACTAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205090 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MITTVTTNSFLLVLGTNYDIQFEFFRTFEVSELFFVSVYVCEFLMKVYVDPITYWKDGYNILDVIILIIL
  TIPYLLRKIKGNHSAYLHFADGIQSLRILKLISYSRGIRTLIIAVGETVYTVASVLTLLFLLMFVFAILG
  FCLFGVTDRGDLENWGNLASAFFTLFSLATVDGWTDLQEELDKRKFTVSRAFTILFILLASFIFLNMFVG
  VMIMHTEDSMKKFERDLTLERNLAIMEEKQIILKRQQEEVNRLMNTQKSGSMNFIDMVEGFKKTLRHTDP
  MVLDDFSTSLSFIDIYLVTLDNQDVIVSKLQELYCEIVNVLSLMLEDMPKESSSSLSGLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC089518
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC089518, AAH89518
• RefSeq Size: 1511 bp
• RefSeq ORF: 1022 bp
• Locus ID: 76856
• Cytogenetics: 13 B1
• MW: 39.1 kDa
• Gene Summary: Voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Activated by intracellular alkalinization.[UniProtKB/Swiss-Prot Function]